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Lrp8 (NM_001080926) Mouse Tagged ORF Clone
CAT＃: MR226275
- TrueORF®
Lrp8 (Myc-DDK-tagged) - Mouse low density lipoprotein receptor-related protein 8, apolipoprotein e receptor (Lrp8), transcript variant 2
ORF Plasmid: tGFP
"NM_001080926" in other vectors (4)
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CNY 9,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4932703M08Rik; AA921429; AI848122; ApoER2; Lr8b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226275 representing NM_001080926
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGCCCAGAACTGGGCGCGCTCCGGCCGCTGGCGCTGTTGCTGTTGCTGCTGCTGCAGCTTCAGC ATCTCTCCGCAGCGGATCCGCTGCCGGGCGGCCAAGGGCCAGTCAAGGAGTGTGAAGAGGACCAGTTTCG GTGTCGGAACGAGCGCTGCATTCCCTTGGTGTGGAGATGCGATGAGGACAACGACTGCTCGGACAACAGC GACGAGGACGACTGCCCCAAGAGGACCTGCGCGGACAGCGACTTCACCTGTGACAATGGCCACTGCATCC CAGAGCGGTGGAAGTGCGACGGCGAGGAGGAGTGTCCCGATGGCTCTGACGAATCGAAGGCCACGTGCTC CAGTGAAGAGTGTCCTGCCGAGAAGTTAAGCTGCGGACCCACCAGCCACAAGTGTGTGCCTGCCTCATGG CGCTGCGACGGAGAGAAGGACTGTGAGGGTGGTGCTGACGAGGCCGGCTGTCCCACCTCAGCACCAGGAC CCTGCCGCGAGAATGAGTTCCAGTGTGGGGATGGGACTTGCGTTCTTGCCATCAAACGGTGCAACCAGGA GCGGGACTGCCCGGACGGGAGTGACGAAGCGGGCTGCCTTCAGGAGTCAACTTGTGAGGGTCCCCGCAGA TTTCAGTGTAAGAGTGGCGAGTGCGTGGACGGCGGGAAAGTGTGTGATGATCAGAGGGACTGCCGGGACT GGTCGGATGAGCCTCAGAAAGTGTGTGGGCTAAATGAGTGTCTGCACAATAACGGCGGCTGTTCCCACAT CTGCACTGACCTCAAGATCGGCTTTGAGTGCACGTGCCCAGCAGGCTTCCAGCTCTTGGACCAGAAGACC TGTGGTGACATTGATGAATGCCAGGACCCGGATGCCTGCAGCCAAATCTGCGTGAATTACAAGGGCTACT TTAAGTGTGAATGCCACCCTGGCTATGAGATGGATACACTGACCAAGAACTGCAAAGCTGTAGCCGGCAA GAGCCCATCTCTGATCTTCACGAACCGACACGAGGTGCGGAGGATAGACCTGGTGAAGCGGGACTACTCG CGCCTCATCCCCATGCTCAAGAATGTCGTGGCGCTGGACGTGGAAGTAGCCACCAATCGCATATACTGGT GCGACCTCTCCTACCGCAAGATCTACAGCGCCCACATGGACAAGGCCAGCATCCCGGACGAGCAGGTGGT CCTCATTGATGAGCAGCTGCACTCCCCGGAGGGCCTGGCGGTGGACTGGGTCCATAAGCACATCTACTGG ACAGACTCAGGCAATAAGACCATCTCAGTGGCCACAACCGATGGACGCCGCCGCTGTACTCTCTTCAGCC GTGAGCTCAGTGAGCCCCGAGCCATTGCCGTTGACCCCCTGCGAGGGTTCATGTACTGGTCTGACTGGGG TTTCCAGGCAAAGATTGAGAAGGCTGGGCTCAATGGTGCAGACCGGCAAACACTGGTTTCAGACAACATT GAATGGCCCAATGGAATCACCCTGGACTTGCTGAGCCAGCGTTTGTACTGGGTGGACTCCAAGCTGCACC AACTATCCAGCATTGACTTCAATGGAGGCAACAGAAAGATGCTGATCTTCTCCACTGACTTCCTGAGTCA CCCTTTTGGGGTTGCTGTATTTGAGGACAAGGTATTCTGGACAGACCTGGAGAATGAGGCCATTTTCAGC GCAAATCGGCTCAATGGCCTGGAAATCGCCATCCTGGCCGAGAACCTCAATAACCCACATGACATTGTAA TCTTCCACGAGCTGAAGCAGCCAAAGGCTGCAGATGCCTGTGACCTGAGTGCCCAGCCCAACGGCGGCTG TGAATACCTGTGCCTTCCTGCTCCTCAAATCTCCAGCCACTCCCCCAAGTACACGTGTGCCTGTCCTGAC ACGATGTGGCTGGGCCCAGACATGAAGAGATGCTACCGAGCACCTCAGTCTACCTCAACTACAACCTTAG CCTCTGCCATGACAAGGACAGTACCTGCTACCACAAGAGCTCCTGGGACAACCATCCACGACCCTACCTA CCAGAACCATAGCACAGAGACGCCAAGCCAGACAGCTGCCGCCCCTCACTCGGTTAACGTCCCTAGGGCT CCCAGCACCAGCCCATCCACCCCGAGCCCTGCTACCAGCAACCACTCCCAGCATTATGGGAATGAAGGCA GCCAGATGGGCTCAACAGTCACCGCTGCTGTCATTGGGGTCATCGTGCCCATAGTGGTAATAGCCCTGCT ATGTATGAGTGGGTACCTCATCTGGAGGAACTGGAAGCGGAAGAACACCAAGAGCATGAATTTCGACAAC CCAGTGTACAGGAAAACGACAGAAGAGGAAGAGGAAGATGAGCTTCACATAGGGAGGACAGCTCAGATTG GCCACGTCTACCCCGCAGCAATCAGCAACTATGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGAC CAGAGACCTGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGAGAACCAAGGTCACAG CTTCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAGTGCAAGCGAGTGGCATTAAGTC TTGAAGATGATGGACTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226275 representing NM_001080926
Red=Cloning site Green=Tags(s) MGRPELGALRPLALLLLLLLQLQHLSAADPLPGGQGPVKECEEDQFRCRNERCIPLVWRCDEDNDCSDNS DEDDCPKRTCADSDFTCDNGHCIPERWKCDGEEECPDGSDESKATCSSEECPAEKLSCGPTSHKCVPASW RCDGEKDCEGGADEAGCPTSAPGPCRENEFQCGDGTCVLAIKRCNQERDCPDGSDEAGCLQESTCEGPRR FQCKSGECVDGGKVCDDQRDCRDWSDEPQKVCGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKT CGDIDECQDPDACSQICVNYKGYFKCECHPGYEMDTLTKNCKAVAGKSPSLIFTNRHEVRRIDLVKRDYS RLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAHMDKASIPDEQVVLIDEQLHSPEGLAVDWVHKHIYW TDSGNKTISVATTDGRRRCTLFSRELSEPRAIAVDPLRGFMYWSDWGFQAKIEKAGLNGADRQTLVSDNI EWPNGITLDLLSQRLYWVDSKLHQLSSIDFNGGNRKMLIFSTDFLSHPFGVAVFEDKVFWTDLENEAIFS ANRLNGLEIAILAENLNNPHDIVIFHELKQPKAADACDLSAQPNGGCEYLCLPAPQISSHSPKYTCACPD TMWLGPDMKRCYRAPQSTSTTTLASAMTRTVPATTRAPGTTIHDPTYQNHSTETPSQTAAAPHSVNVPRA PSTSPSTPSPATSNHSQHYGNEGSQMGSTVTAAVIGVIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDN PVYRKTTEEEEEDELHIGRTAQIGHVYPAAISNYDRPLWAEPCLGETRDLEDPAPALKELFVLPGEPRSQ LHQLPKNPLSELPVVKCKRVALSLEDDGLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080926 |
| ORF Size | 2610 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080926.1, NP_001074395.1 |
| RefSeq Size | 7296 bp |
| RefSeq ORF | 2613 bp |
| Locus ID | 16975 |
| MW | 96.8 kDa |
| Gene Summary | Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation (PubMed:12695510). May also function as an endocytic receptor. Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2 (PubMed:18174160). Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells (PubMed:29336888).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222314 | Lrp8 (untagged) - Mouse low density lipoprotein receptor-related protein 8, apolipoprotein e receptor (Lrp8), transcript variant 2, (10ug) |
CNY 9,568.00 |
|
| MG226275 | Lrp8 (tGFP-tagged) - Mouse low density lipoprotein receptor-related protein 8 apolipoprotein e receptor (Lrp8) transcript variant 2, (10ug) |
CNY 11,160.00 |
|
| MR226275L3 | Lenti ORF clone of Lrp8 (Myc-DDK-tagged) - Mouse low density lipoprotein receptor-related protein 8, apolipoprotein e receptor (Lrp8), transcript variant 2 |
CNY 10,550.00 |
|
| MR226275L4 | Lenti ORF clone of Lrp8 (mGFP-tagged) - Mouse low density lipoprotein receptor-related protein 8, apolipoprotein e receptor (Lrp8), transcript variant 2 |
CNY 11,960.00 |
