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Trpa1 (NM_177781) Mouse Tagged ORF Clone
CAT＃: MR227099
- TrueORF®
Trpa1 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1)
ORF Plasmid: tGFP
"NM_177781" in other vectors (6)
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CNY 11,760.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Anktm1; TRPA1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227099 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGCGGCTTGAGGAGGATTCTGCTCCCGGAGGAAAGGAAGGAGGTCCAGGGCGTTGTCTATCGCG GCGTCGGGGAAGACATGGACTGCTCCAAGGAATCCTTTAAGGTGGACATTGAAGGAGATATGTGTAGATT AGAAGACTTCATCAAGAACCGAAGAAAACTAAGCAAATATGAGGATGAAAATCTCTGTCCTCTGCATCAC GCAGCAGCAGAAGGTCAAGTTGAACTGATGGAACTGATCATCAATGGTTCTTCGTGTGAAGTGCTGAATA TAATGGATGGTTATGGAAATACCCCACTGCATTGTGCTGCAGAAAAAAATCAAGTTGAAAGTGTAAAGTT TCTTCTCAGCCAAGGAGCAAATCCAAACCTCCGAAATAGAAACATGATGTCACCCCTTCACATAGCTGTG CATGGCATGTACAACGAAGTGATCAAGGTGTTGACTGAGCACAAGGCCACTAACATCAATTTAGAAGGAG AGAATGGGAACACGGCTTTGATGTCCACGTGTGCCAAAGACAACAGTGAAGCTTTGCAAATTTTGTTAGA AAAAGGAGCTAAGCTGTGTAAATCAAATAAGTGGGGAGACTACCCTGTGCACCAGGCAGCATTTTCAGGT GCCAAAAAATGCATGGAATTAATCTTAGCATATGGTGAAAAGAACGGCTACAGCAGGGAGACTCACATTA ATTTTGTGAATCACAAGAAAGCCAGCCCTCTCCACCTAGCAGTTCAAAGCGGAGACTTGGACATGATTAA GATGTGCCTGGACAACGGTGCACACATCGACATGATGGAGAATGCCAAATGCATGGCCCTCCATTTTGCT GCAACCCAGGGAGCCACTGACATCGTTAAGCTCATGATCTCATCCTATACCGGAAGTAGTGATATTGTGA ATGCAGTTGATGGCAATCAGGAGACCCTGCTTCACAGAGCCTCGTTATTTGATCACCATGACCTGGCAGA ATACCTAATATCAGTGGGAGCAGACATCAACAGCACTGATTCTGAAGGACGCTCTCCACTTATTTTAGCA ACAGCTTCTGCATCCTGGAACATTGTGAATTTGCTCCTCTGTAAAGGTGCCAAAGTAGACATAAAAGATC ATCTTGGACGTAACTTTTTGCATTTGACTGTGCAGCAGCCTTATGGACTAAGAAATTTGCGGCCTGAGTT TATGCAGATGCAACACATCAAAGAGCTGGTGATGGATGAAGACAATGACGGATGCACACCTCTCCATTAT GCCTGTAGGCAGGGGGTTCCTGTCTCTGTAAATAACCTCCTTGGCTTCAATGTGTCCATTCATAGCAAAA GTAAAGATAAGAAGTCGCCCCTGCATTTTGCAGCCAGTTATGGGCGCATCAATACATGTCAGAGACTTCT GCAAGACATAAGTGATACGAGGCTTTTGAATGAAGGGGATCTCCATGGGATGACCCCTCTCCACCTGGCA GCAAAAAATGGGCATGATAAAGTCGTTCAACTCCTTCTGAAGAAAGGGGCCTTATTTCTCAGTGACCACA ATGGCTGGACTGCTTTGCATCACGCCTCCATGGGTGGGTACACTCAGACCATGAAGGTCATTCTTGATAC TAACTTGAAATGCACAGACCGACTAGATGAAGAAGGGAACACAGCACTCCACTTTGCAGCACGGGAAGGC CATGCCAAGGCTGTTGCAATGCTTTTGAGCTACAATGCTGACATCCTCCTGAACAAGAAGCAAGCTTCCT TTCTGCATATTGCCCTGCACAATAAGCGCAAGGAAGTGGTTCTCACAACCATCAGAAATAAAAGATGGGA TGAGTGTCTTCAAGTTTTCACTCATAATTCTCCAAGCAATCGATGTCCAATCATGGAGATGGTAGAATAC CTCCCCGAGTGCATGAAAGTTCTTTTAGATTTCTGCATGATACCTTCCACAGAAGACAAGTCCTGTCAAG ACTACCATATTGAGTATAATTTCAAGTATCTCCAATGCCCATTATCCATGACCAAAAAAGTAGCACCTAC CCAGGATGTGGTATATGAGCCTCTTACAATCCTCAATGTCATGGTCCAACATAACCGCATAGAACTCCTC AACCACCCTGTGTGTAGGGAGTACTTACTCATGAAATGGTGTGCCTATGGATTCAGAGCCCATATGATGA ACCTAGGATCTTATTGTCTTGGTCTCATACCCATGACCCTTCTTGTTGTCAAAATACAGCCTGGAATGGC CTTCAATTCTACTGGAATAATCAATGGAACTAGTAGTACTCATGAGGAAAGAATAGACACTCTGAATTCA TTTCCAATAAAAATATGTATGATTCTAGTTTTTTTATCAAGTATATTTGGATATTGCAAAGAAGTGATCC AAATTTTCCAACAGAAAAGGAATTACTTCCTGGATTACAACAATGCTCTGGAATGGGTTATCTATACAAC TAGTATCATCTTCGTGTTGCCCTTGTTCCTCAACATCCCAGCGTATATGCAGTGGCAATGTGGAGCAATA GCGATATTCTTCTACTGGATGAACTTCCTACTGTATCTTCAAAGGTTTGAGAACTGTGGAATTTTCATTG TTATGTTGGAGGTGATTTTTAAAACATTGCTGAGATCGACCGGAGTGTTTATCTTCCTCCTACTGGCTTT TGGCCTCAGCTTTTATGTTCTCCTGAATTTCCAAGATGCCTTCAGCACCCCATTGCTTTCCTTAATCCAG ACATTCAGTATGATGCTAGGAGACATCAATTATCGAGATGCCTTCCTAGAACCATTGTTTAGAAATGAGT TGGCATACCCAGTCCTGACCTTTGGGCAGCTTATTGCCTTCACAATGTTTGTCCCAATTGTTCTCATGAA CTTACTGATTGGCTTGGCGGTTGGGGACATTGCTGAGGTCCAGAAGCATGCGTCATTGAAGAGGATTGCT ATGCAGGTGGAACTTCATACCAACTTAGAAAAAAAGCTGCCACTCTGGTACTTACGCAAAGTGGATCAGA GGTCCACCATCGTGTATCCAAATAGACCCAGGCACGGCAGGATGCTACGGTTTTTTCATTACTTTCTTAA TATGCAAGAAACACGACAAGAAGTACCAAACATTGACACATGCTTGGAAATGGAAATATTGAAACAGAAA TATCGGCTGAAGGACCTCACTTCCCTCTTGGAAAAGCAGCATGAGCTCATCAAACTCATCATCCAGAAGA TGGAGATCATCTCAGAGACAGAAGATGAAGATAACCATTGCTCTTTCCAAGACAGGTTCAAGAAGGAGAG GCTGGAACAGATGCACAGCAAGTGGAATTTTGTCTTAAACGCAGTTAAGACTAAAACACATTGTTCTATT AGCCACCCGGACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227099 protein sequence
Red=Cloning site Green=Tags(s) MKRGLRRILLPEERKEVQGVVYRGVGEDMDCSKESFKVDIEGDMCRLEDFIKNRRKLSKYEDENLCPLHH AAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAV HGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSG AKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFA ATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILA TASASWNIVNLLLCKGAKVDIKDHLGRNFLHLTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHY ACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLA AKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEEGNTALHFAAREG HAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEY LPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQCPLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELL NHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIINGTSSTHEERIDTLNS FPIKICMILVFLSSIFGYCKEVIQIFQQKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAI AIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQ TFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIA MQVELHTNLEKKLPLWYLRKVDQRSTIVYPNRPRHGRMLRFFHYFLNMQETRQEVPNIDTCLEMEILKQK YRLKDLTSLLEKQHELIKLIIQKMEIISETEDEDNHCSFQDRFKKERLEQMHSKWNFVLNAVKTKTHCSI SHPDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177781 |
| ORF Size | 3378 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177781.5 |
| RefSeq Size | 4263 bp |
| RefSeq ORF | 3378 bp |
| Locus ID | 277328 |
| UniProt ID | Q8BLA8 |
| MW | 128.5 kDa |
| Gene Summary | Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, cinnamaldehyde, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Is also activated by menthol (in vitro) (By similarity). May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A critical evaluation of TRPA1-mediated locomotor behavior in zebrafish as a screening tool for novel anti-nociceptive drug discovery
,null,
Scientific Reports
,PubMed ID 30787340
[Trpa1]
|
|
Phosphorylation of the Transient Receptor Potential Ankyrin 1 by Cyclin-dependent Kinase 5 affects Chemo-nociception
,Hall, BE;Prochazkova, M;Sapio, MR;Minetos, P;Kurochkina, N;Binukumar, BK;Amin, ND;Terse, A;Joseph, J;Raithel, SJ;Mannes, AJ;Pant, HC;Chung, MK;Iadarola, MJ;Kulkarni, AB;,
Sci Rep
,PubMed ID 29352128
[Trpa1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223667 | Trpa1 (untagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1), (10ug) |
CNY 12,544.00 |
|
| MG227099 | Trpa1 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily A member 1 (Trpa1), (10ug) |
CNY 13,360.00 |
|
| MR227099L1 | Lenti ORF clone of Trpa1 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1) |
CNY 13,110.00 |
|
| MR227099L2 | Lenti ORF clone of Trpa1 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1) |
CNY 14,160.00 |
|
| MR227099L3 | Lenti ORF clone of Trpa1 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1) |
CNY 13,110.00 |
|
| MR227099L4 | Lenti ORF clone of Trpa1 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1) |
CNY 13,110.00 |
