Camk1d (NM_001290374) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A630059D12Rik; CaMKIdelta; CKLiK; E030025C11Rik; mCKLiK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229326 representing NM_001290374 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGGAGAACGGCGAGAGCAGCTCCTCCTGGAAAAAGCAAGCAGAAGACATTAAGAAGATCTTCG AGTTCAAGGAGACCCTCGGAACTGGGGCCTTTTCTGAAGTTGTTTTAGCCGAGGAGAAAGCTACTGGGAA GCTCTTCGCAGTGAAGTGCATCCCGAAGAAGGCGCTGAAGGGCAAGGAGAGCAGCATCGAGAACGAGATT GCCGTGCTTAGAAATGTGTCTGGTGGAGAACTCTTCGATCGGATAGTGGAGAAGGGGTTTTACACAGAGA AAGATGCCAGCACTCTCATCCGCCAGGTCCTGGATGCCGTATACTATCTCCACAGAATGGGCATTGTCCA CAGGGACCTCAAGCCGGAGAATCTCTTATACTACAGTCAAGACGAGGAGTCCAAAATAATGATCAGTGAC TTTGGCTTGTCGAAAATGGAGGGCAAAGGAGATGTGATGTCCACGGCCTGCGGGACCCCAGGCTATGTTG CTCCGGAAGTTCTCGCCCAGAAACCGTACAGCAAAGCTGTGGACTGCTGGTCCATCGGGGTGATCGCCTA TATCTTGCTCTGTGGTTACCCTCCTTTTTATGATGAAAATGACTCGAAGCTGTTTGAACAGATCCTCAAG GCAGAATATGAGTTTGATTCCCCCTACTGGGATGACATCTCCGACTCTGCCAAAGACTTCATTCGGAATC TGATGGAGAAAGACCCAAATAAAAGATACACTTGTGAGCAGGCAGCTCGACACCCATGGATTGCTGGTGA CACAGCCCTTAGCAAAAACATTCACGAATCTGTCAGTGCCCAGATCCGGAAGAATTTTGCAAAGAGCAAA TGGAGACAAGCGTTTAACGCCACGGCAGTCGTGAGACATATGCGGAGGCTCCAGCTTGGCAGCAGCCTGG ACAGTTCAAATGTCTGGCACCTTCCACGCTCTGTAGTTTCCTTTCTTCTTCGTCGGGGGTCGCAGGAGTC GGAGCTGAGAGGAGACCCAGGCCCACCACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229326 representing NM_001290374 Red=Cloning site Green=Tags(s) MARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEI AVLRNVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISD FGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDSKLFEQILK AEYEFDSPYWDDISDSAKDFIRNLMEKDPNKRYTCEQAARHPWIAGDTALSKNIHESVSAQIRKNFAKSK WRQAFNATAVVRHMRRLQLGSSLDSSNVWHLPRSVVSFLLRRGSQESELRGDPGPPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290374
ORF Size	1011 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290374.1, NP_001277303.1
RefSeq Size	7108 bp
RefSeq ORF	1014 bp
Locus ID	227541
UniProt ID	Q8BW96
MW	38.5 kDa
Gene Summary	Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta (By similarity). Isoform 1 but not isoform 2 activates CREB1.[UniProtKB/Swiss-Prot Function]

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