Trpc6 (NM_001282087) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AV025995; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; TRP-6; Trrp6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229746 representing NM_001282087 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGAGCCCGAGGTTCGTGACCCGGAGGGGCGGCTCTCTAAAGGCTGCCCCTGGAGCCGGCACCC GGCGCAACGAGAGCCAGGACTATTTGCTGATGGACGAGCTGGGAGACGACGGCTACCCGCAGCTCCCGCT GCCACCGTATGGCTACTACCCCAGCTTCCGGGGTAATGAAAACAGACTGACTCACCGGCGGCAGACGATT CTTCGTGAGAAGGGAAGAAGGTTAGCTAATCGAGGACCAGCATACATGTTTAATGATCATTCAACAAGCC TGTCTATTGAGGAAGAACGCTTTCTAGATGCAGCTGAATATGGCAACATCCCAGTGGTGCGGAAGATGCT AGAAGAGTGTCATTCCCTCAATGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGCTGGCTGTG GCCAATGAGCACTTGGAAATCACAGAGCTGCTACTCAAGAAGGAAAACTTGTCTCGAGTTGGGGATGCTT TACTTTTAGCCATTAGTAAAGGTTATGTACGGATTGTGGAGGCAATCCTCAACCATCCAGCTTTTGCTGA AGGCAAAAGGTTAGCGACAAGCCCCAGCCAGTCTGAACTTCAGCAAGATGACTTTTATGCCTATGATGAA GATGGGACGCGGTTCTCCCATGATGTGACCCCAATCATTCTCGCTGCACATTGCCAGGAATATGAAATTG TGCATACCCTCCTGAGAAAGGGTGCCCGGATTGAGCGGCCTCATGATTACTTCTGCAAGTGTACAGAATG CAGCCAGAAGCAGAAGCATGATTCCTTCAGCCACTCTAGATCCAGGATCAATGCATACAAAGGTCTGGCA AGTCCAGCATACCTGTCATTGTCCAGTGAAGATCCAGTCATGACTGCTTTAGAACTTAGCAATGAGCTGG CAGTGCTTGCCAACATTGAGAAAGAGTTCAAGAATGACTACAGGAAGCTGTCTATGCAGTGCAAGGATTT CGTTGTTGGTCTCTTGGACCTCTGCAGAAACACAGAGGAAGTGGAGGCCATCCTGAATGGGGATGCAGAG ACTCGCCAGCCCGGGGACTTCGGCCGTCCAAATCTCAGCCGTTTAAAACTTGCTATTAAGTATGAAGTAA AAAAAGTAAGGCAGGGCGTGAGGCCCCTTTGTTACTTCAGTTTGTGTGTGTGGATATGCCAAGCTGGAAA GAGCAGGGGGTCATCCCGAGGAAGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229746 representing NM_001282087 Red=Cloning site Green=Tags(s) MSQSPRFVTRRGGSLKAAPGAGTRRNESQDYLLMDELGDDGYPQLPLPPYGYYPSFRGNENRLTHRRQTI LREKGRRLANRGPAYMFNDHSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAV ANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILNHPAFAEGKRLATSPSQSELQQDDFYAYDE DGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCTECSQKQKHDSFSHSRSRINAYKGLA SPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYRKLSMQCKDFVVGLLDLCRNTEEVEAILNGDAE TRQPGDFGRPNLSRLKLAIKYEVKKVRQGVRPLCYFSLCVWICQAGKSRGSSRGSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282087
ORF Size	1218 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282087.1, NP_001269016.1
RefSeq Size	3235 bp
RefSeq ORF	1221 bp
Locus ID	22068
MW	46.4 kDa
Gene Summary	Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.[UniProtKB/Swiss-Prot Function]

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