Grk2 (NM_001290818) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Adrbk-1; Bark-1; beta ARK; betaARK1; GRK2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230892 representing NM_001290818 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACCTGGAGGCGGTGCTGGCCGATGTGAGCTACCTGATGGCCATGGAGAAGAGCAAGGCCACGC CGGCCGCGCGCGCCAGCAAGAAGATCCTGCTGCCAGAGCCCAGCATCCGCAGCGTCATGCAGAAGTATCT GGAGGACCGAGGAGAAGTGACCTTTGAAAAGATCTTTTCGCAGAAGTTAGGGTACCTGCTTTTCCGGGAC TTCTGCCTGAACCATCTGGAAGAGGCCAAGCCCTTGGTGGAGTTCTACGAAGAGATCAAGAAATATGAGA AGCTGGAGACAGAGGAGGAACGTGTGGTCCGCAGCCGAGAGATCTTTGACTCCTATATTATGAAAGAACT GCTGGCCTGCTCACATCCCTTTTCAAAGAATGCTACGGAGCATGTCCAGGGCCACCTGGTGAAGAAGCAG GTGCCTCCAGATCTCTTCCAGCCATACATTGAGGAGATTTGTCAGAACCTCCGAGGGGACGTGTTCCAGA AGTTCATAGAGAGTGACAAGTTCACACGGTTCTGCCAGTGGAAGAATGTTGAGCTCAACATCCACCTGAC CATGAATGACTTCAGTGTGCATCGAATCATCGGGCGTGGGGGCTTCGGTGAGGTCTATGGGTGCCGGAAA GCAGACACAGGCAAGATGTACGCCATGAAGTGTCTGGACAAGAAACGCATCAAGATGAAGCAGGGAGAGA CCCTGGCTCTGAACGAGCGGATCATGCTTTCCCTCGTCAGCACCGGGGACTGCCCCTTCATCGTGTGCAT GTCATATGCATTCCACACACCAGACAAGCTCAGCTTCATCCTGGACCTCATGAACGGTGGGGACCTGCAC TACCACCTGTCTCAGCATGGAGTCTTCTCTGAAGCCGACATGCGCTTCTACGCCGCTGAGATCATCCTGG GCCTTGAACACATGCACAATCGCTTTGTAGTCTACAGGGACCTGAAGCCAGCCAACATTCTCCTGGATGA ACATGGCCATGTGAGAATCTCGGACCTGGGCCTAGCCTGTGACTTCTCCAAGAAGAGGCCTCATGCCAGT GTGGGCACACACGGGTACATGGCCCCCGAAGTCCTACAGAAGGGCGTGGCCTATGACAGCAGTGCAGACT GGTTCTCCCTGGGCTGCATGCTCTTCAAGTTGTTGCGGGGACACAGCCCCTTCCGGCAGCACAAGACCAA AGACAAGCATGAGATTGACCGCATGACGTTGACGATGGCTGTAGAGCTGCCTGACTCCTTCTCCCCTGAA CTCCGATCCCTGCTGGAAGGTTTGTTGCAGAGGGACGTCAATCGGAGACTAGGCTGTCTGGGCCGTGGGG CTCAGGAGGTAAAAGAAAGTCCTTTCTTTCGTTCCCTGGACTGGCAGATGGTCTTCTTACAGAAGTACCC TCCTCCACTGATCCCCCCACGTGGGGAGGTGAATGCAGCTGACGCTTTCGACATTGGCTCCTTTGATGAG GAGGACACAAAAGGAATCAAGTTACTGGACAGTGACCAGGAACTGTACCGCAACTTCCCTCTCACCATCT CTGAGCGGTGGCAACAGGAGGTGGCAGAGACTGTCTTTGATACCATCAATGCTGAAACAGACCGGCTGGA GGCACGAAAGAAGGCCAAAAACAAACAGCTGGGCCACGAGGAGGACTATGCCCTGGGTAAGGACTGCATC GTGCATGGCTACATGTCCAAGATGGGCAACCCCTTCCTGACCCAGTGGCAGCGGCGATACTTCTACTTGT TCCCCAACCGGCTCGAGTGGAGGGGTGAAGGCGAGGCTCCGCAGAGCCTCCTGACCATGGAGGAGATCCA GTCAGTGGAAGAGACACAGATCAAGGAACGCAAGTGTCTCCTGCTTAAGATCCGAGGTGGCAAGCAGTTT GTCCTGCAGTGTGATAGTGATCCAGAGCTGGTGCAATGGAAAAAGGAGCTGCGTGATGCCTACCGCGAGG CCCAGCAGCTGGTACAGCGAGTACCCAAGATGAAGAACAAGCCACGCTCGCCTGTGGTGGAGCTGAGCAA GGTGCCGCTGATCCAGCGTGGCAGTGCCAACGGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230892 representing NM_001290818 Red=Cloning site Green=Tags(s) MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRD FCLNHLEEAKPLVEFYEEIKKYEKLETEEERVVRSREIFDSYIMKELLACSHPFSKNATEHVQGHLVKKQ VPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRK ADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLH YHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKRPHAS VGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPE LRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDE EDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCI VHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQF VLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLIQRGSANGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290818
ORF Size	2067 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290818.1, NP_001277747.1
RefSeq Size	3379 bp
RefSeq ORF	2070 bp
Locus ID	110355
UniProt ID	Q99MK8
MW	80.1 kDa
Gene Summary	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity.[UniProtKB/Swiss-Prot Function]

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