Wwp1 (NM_001276292) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	8030445B08Rik; AIP5; SDRP1; Tiul1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231145 representing NM_001276292 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACGAGCAGCTGATTGTAAATGTGACCCCACAGACCACCTTGGAGTTCCGAGTTTGGAGTCACCAC ACTTTAAAGGCAGATGCTCTATTAGGAAAAGCAACAGTGGACTTGAAACAAGTGCTGTTAACACACAACA GAAAATGTTGCCTGTTGAAGGTACTATTGGAATTGATAATCATGTATCAACCAATACTGTTGTTCCCAAC TCCTGCTGTTCACATGTAGTTAATGGAGAAAACACACCTTCATCCCCGTCTCAGGTTGCTGCCAGACCCA AAAATGCTCCAGCTCCAAAGCCAGTCACATCTGCGCCCACCAGTGACACGGTTAATGGAGAATCATCATC TGTCCTAGCTGATAATACTTCAACCATGGGTACTTTACTACCATCTGAAGATACCACCTCGACTTCCAAT TGCACTAGCACCACCACCCAAGAGCCTCCTGTACAAGAACCCCCAGCATCCTCAGAACACAGTGAATGTA TTCCCTCTGCCAGTGCGGAAGTGGGACCAGAAGCTCGGAGCCTCATAGACCCTGACTCTGATTCTAGAAA TAATTCTGTGTTTGACAAAGTAAGGCAGCCAGAAGGGTGTGTAGAACCTCTGCGGCCACAGTCTGGAAAC ACCAACACAGAAGCTTTGCCATCAGGGTGGGAACAGAGAAAAGATCCTCATGGTAGAACCTATTATGTGG ATCATAATACTCGAACTACTACATGGGAGAGACCACAGCCTTTGCCTCCAGGTTGGGAAAGAAGAGTTGA TGATCGTGGAAGAGTTTATTATGTGGATCATAACACCAGAACAACAACCTGGCAGCGGCCTACTATGGAA TCTGTTCGAAATTTTGAACAGTGGCAGTCTCAGCGGAATCAATTGCAGGGAGCTATGCAACAATTCAACC AACGATACCTCTATTCGGCTTCCATGTTAGCTGCAGAAAATGACCCCTATGGACCCTTACCACCGGGCTG GGAAAAAAGAGTGGATTCAACAGACAGAGTTTACTTTGTGAATCACAATACAAAAACAACCCAGTGGGAA GATCCAAGAACTCAAGGCTTACCAAATGAAGAACCCCTGCCCGAAGGCTGGGAAATCAGGTATACTCGGG AAGGTGTTAGGTACTTTGTTGATCATAACACGAGAACAACAACATTCAAAGACCCTCGCAATGGGAAGTC ATCAGTAACTAAAGGTGGTCCACAGATTGCTTATGAACGCAGCTTTAGGTGGAAGCTTGCTCACTTCCGT TACTTGTGCCAGTCTAATGCACTACCGAGTCATGTAAAGATCAATGTGTCCCGACAGACATTATTTGAAG ATTCCTTCCAACAGATTATGGCATTAAAACCATATGACTTGAGGAGGCGATTATATGTAATATTTAGAGG AGAAGAAGGTCTTGATTATGGTGGCCTTGCAAGAGAATGGTTTTTCTTGCTTTCCCATGAAGTTTTGAAC CCAATGTATTGCTTATTTGAGTATGCAGGCAAGAATAACTATTGTTTACAGATAAATCCAGCATCAACTA TAAATCCTGACCATCTTTCATACTTCTGTTTTATTGGTCGCTTTATTGCAATGGCACTGTTCCATGGAAA GTTTATTGATACTGGTTTCTCTTTACCATTCTACAAGCGTATGCTAAGTAAAAAACTTACTATTAAGGAT TTGGAATCTATTGATACTGAATTTTACAACTCTCTAATCTGGATAAGAGATAACAATATTGAAGAATGTG GCTTAGAAATGTACTTTTCTGTAGACATGGAGATTCTGGGAAAAGTTACTTCACATGATTTAAAGTTGGG AGGTTCCAATATCCTGGTGACGGAAGAAAACAAAGATGAATATATTGGTTTAATGACAGAATGGCGTTTT TCTCGAGGAGTACAAGAACAGACCAAAGCTTTCCTTGATGGGTTTAATGAAGTTGTTCCCCTTCAGTGGC TACAGTACTTTGATGAAAAAGAATTGGAGGTTATGCTGTGTGGCATGCAAGAGGTTGACTTGGCAGACTG GCAGAGAAATACTGTTTATCGACATTATACAAGAAACAGCAAGCAAATTATTTGGTTTTGGCAGTTTGTG AAAGAGACTGATAATGAAGTACGAATGCGACTCTTGCAGTTTGTGACTGGAACCTGTCGCTTGCCTCTAG GAGGATTTGCTGAGCTCATGGGAAGTAATGGGCCTCAAAAGTTCTGTATTGAAAAAGTTGGCAAAGACAC CTGGTTACCAAGAAGCCATACATGTTTTAATCGCTTGGATCTACCACCATATAAGAGTTATGAACAACTA AAGGAAAAACTTCTTTTTGCAATAGAAGAGACAGAGGGATTTGGACAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231145 representing NM_001276292 Red=Cloning site Green=Tags(s) MGRAADCKCDPTDHLGVPSLESPHFKGRCSIRKSNSGLETSAVNTQQKMLPVEGTIGIDNHVSTNTVVPN SCCSHVVNGENTPSSPSQVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSN CTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGN TNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTME SVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWE DPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFR YLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLN PMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKD LESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRF SRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFV KETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQL KEKLLFAIEETEGFGQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276292
ORF Size	2361 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276292.1, NP_001263221.1
RefSeq Size	6296 bp
RefSeq ORF	2364 bp
Locus ID	107568
UniProt ID	Q8BZZ3
MW	90.5 kDa
Gene Summary	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF (By similarity). Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7.[UniProtKB/Swiss-Prot Function]

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