Dnm2 (NM_001253893) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	b2b2159Clo; Dyn2; Udnm
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231296 representing NM_001253893 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACCGCGGGATGGAAGAGCTCATCCCGCTGGTTAACAAGCTGCAGGACGCCTTCAGCTCCATCG GTCAGAGCTGCCACCTGGACCTGCCGCAGATCGCCGTGGTGGGCGGCCAGAGCGCCGGCAAAAGTTCGGT GCTCGAGAACTTCGTGGGCCGGGACTTCCTTCCACGAGGATCAGGAATTGTCACCCGGAGGCCTCTCATT CTGCAGCTCATCTTTTCCAAAACAGAATATGCGGAATTTTTGCACTGCAAGTCCAAAAAATTTACAGACT TTGATGAAGTCCGGCAGGAGATCGAAGCAGAGACTGACCGGGTCACAGGCACCAACAAAGGCATCTCCCC TGTGCCCATCAACCTTCGGGTCTACTCACCACACGTGTTGAACTTGACCCTCATCGACCTCCCAGGCATC ACTAAGGTGCCGGTGGGGGACCAGCCGCCAGACATCGAGTACCAGATCAAGGACATGATCCTGCAGTTCA TCAGCCGCGAGAGCAGCCTCATTCTTGCCGTCACACCTGCCAACATGGACTTGGCCAACTCAGACGCCCT CAAGCTGGCCAAGGAGGTGGACCCCCAAGGCCTACGGACCATCGGTGTCATCACCAAGCTAGACTTGATG GATGAAGGCACAGACGCCAGGGATGTCCTGGAAAACAAGCTACTGCCCTTGAGAAGAGGCTATATCGGCG TGGTTAACCGAAGCCAGAAAGACATCGAGGGCAAAAAGGACATCCGGGCTGCTCTGGCAGCCGAGAGGAA ATTCTTCCTCTCCCACCCAGCCTACCGGCACATGGCTGACCGCATGGGCACCCCACACTTGCAGAAAACC CTGAACCAGCAACTGACCAACCACATCCGAGAGTCACTGCCGACCCTTCGCAGCAAGCTGCAGAGCCAAC TGCTGTCCCTGGAGAAGGAAGTGGAAGAGTACAAGAATTTCCGGCCTGATGACCCCACGCGCAAGACCAA AGCCCTGCTGCAGATGGTTCAGCAGTTTGGAGTGGACTTTGAGAAGCGAATTGAAGGCTCGGGAGATCAA GTAGACACACTAGAGTTGTCTGGTGGAGCCCGCATCAATCGTATCTTTCATGAGCGCTTTCCCTTTGAAC TGGTAAAGATGGAGTTTGATGAGAAAGATCTACGAAGAGAGATCAGCTATGCTATTAAGAACATCCACGG AGTCAGAACTGGGCTCTTCACGCCGGATTTGGCGTTCGAGGCCATTGTGAAAAAGCAGGTGGTCAAGCTG AAAGAGCCCTGTCTGAAATGCGTGGACCTGGTTATCCAGGAGCTAATCAGTACAGTTAGGCAGTGCACCA GCAAGCTGAGTTCCTACCCCCGGCTGCGAGAGGAGACCGAGCGAATTGTCACCACCTACATCAGGGAGCG AGAAGGGAGAACCAAGGACCAGATTCTTCTTCTGATTGACATTGAGCAGTCGTACATCAACACAAACCAT GAAGACTTCATTGGATTTGCCAATGCCCAGCAGAGGAGCACGCAGCTGAACAAGAAGAGGGCCATACCCA ATCAGGGGGAGATCTTGGTGATCCGCAGGGGCTGGTTGACCATCAACAATATCAGCTTGATGAAGGGTGG CTCCAAGGAGTACTGGTTCGTGCTGACAGCTGAGTCATTGTCTTGGTACAAGGATGAGGAGGAGAAAGAA AAGAAGTACATGCTGCCACTGGACAACCTCAAGATCCGGGACGTGGAAAAGGGCTTCATGTCCAACAAGC ACGTGTTTGCCATCTTCAACACAGAGCAGAGGAATGTCTACAAGGACCTTCGACAGATTGAACTGGCTTG TGACTCCCAGGAAGATGTGGACAGCTGGAAGGCTTCGTTCCTGCGCGCTGGGGTCTACCCAGAGAAGGAC CAGGCAGAGAATGAGGATGGAGCACAAGAGAACACCTTCTCCATGGACCCGCAGCTGGAGCGACAGGTGG AGACCATCCGTAACCTGGTGGACTCCTACGTGGCCATCATCAACAAGTCCATCCGTGACCTTATGCCAAA GACCATCATGCACCTCATGATCAACAACACAAAGGCCTTCATCCACCATGAGCTGCTGGCTTACCTGTAT TCATCAGCAGACCAGAGCAGCCTCATGGAAGAGTCAGCCGAGCAGGCTCAGCGGCGGGACGACATGCTGC GCATGTACCACGCACTGAAGGAGGCCCTCAACATTATTGGGGACATCAGCACCAGCACCGTGTCCACACC CGTGCCCCCACCGGTCGATGACACGTGGCTCCAAAACACCAGCGGCCACAGCCCCACTCCACAGCGCCGA CCTGTGTCCAGTGTGCACCCACCAGGCCGGCCACCTGCAGTGCGGGGTCCTACACCGGGGCCTCCCCTGA TTCCTATGCCTGTGGGGGCCACATCCTCCTTCTCAGCACCTCCCATCCCATCCCGGCCTGGACCACAGAG TGTGTTTGCCAACAACGACCCCTTCTCTGCGCCACCTCAGATACCATCTCGGCCAGCACGGATTCCCCCT GGTATCCCTCCAGGAGTGCCCAGCAGAAGAGCGCCCGCTGCGCCCAGCCGGCCCACCATTATCCGCCCAG CCGAGCCGTCCCTGCTCGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231296 representing NM_001253893 Red=Cloning site Green=Tags(s) MGNRGMEELIPLVNKLQDAFSSIGQSCHLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLI LQLIFSKTEYAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGI TKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLM DEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEGKKDIRAALAAERKFFLSHPAYRHMADRMGTPHLQKT LNQQLTNHIRESLPTLRSKLQSQLLSLEKEVEEYKNFRPDDPTRKTKALLQMVQQFGVDFEKRIEGSGDQ VDTLELSGGARINRIFHERFPFELVKMEFDEKDLRREISYAIKNIHGVRTGLFTPDLAFEAIVKKQVVKL KEPCLKCVDLVIQELISTVRQCTSKLSSYPRLREETERIVTTYIREREGRTKDQILLLIDIEQSYINTNH EDFIGFANAQQRSTQLNKKRAIPNQGEILVIRRGWLTINNISLMKGGSKEYWFVLTAESLSWYKDEEEKE KKYMLPLDNLKIRDVEKGFMSNKHVFAIFNTEQRNVYKDLRQIELACDSQEDVDSWKASFLRAGVYPEKD QAENEDGAQENTFSMDPQLERQVETIRNLVDSYVAIINKSIRDLMPKTIMHLMINNTKAFIHHELLAYLY SSADQSSLMEESAEQAQRRDDMLRMYHALKEALNIIGDISTSTVSTPVPPPVDDTWLQNTSGHSPTPQRR PVSSVHPPGRPPAVRGPTPGPPLIPMPVGATSSFSAPPIPSRPGPQSVFANNDPFSAPPQIPSRPARIPP GIPPGVPSRRAPAAPSRPTIIRPAEPSLLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253893
ORF Size	2610 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253893.1, NP_001240822.1
RefSeq Size	3582 bp
RefSeq ORF	2613 bp
Locus ID	13430
UniProt ID	P39054
MW	98.6 kDa
Gene Summary	Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis (PubMed:18923138). Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (PubMed:18425118).[UniProtKB/Swiss-Prot Function]

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