Asap1 (NM_001276467) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231570 representing NM_001276467 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGATCTTCAGCCTCCCGGCTCTCCAGTTTTTCCTCCAGAGATTCACTATGGAATCGGATGCCGGACC AGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCAC CACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCC CTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAAAATGAAGAAA ACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACCTTGGCACCGC TTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCAGGGTCTGAGC CACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGAGATCTCAAGA AGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGAAAAGAGAGCA CGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAATGGAAAAGGAA CGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACCAAAAAGGGTG TGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATGGCTTGAAAAC GGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCAAACCCAAGAT GAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGATCCGAAAGAAG TAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTTCTAGAAGAGACTCTCAGAGCCGGCAAGGTGGGTA CAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGT GATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCACG CAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTCCTCACCTGCCAAGTGAAACCGAATGCTGAGGACAA GAAGTCTTTTGACCTGATATCACATAATCGAACATATCACTTTCAAGCAGAAGATGAGCAGGATTATATA GCGTGGATATCAGTACTGACGAATAGCAAAGAAGAGGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGCA CAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCCATCATCGAGGATGTACAGCGGCTCCCTGGGAATGA CATCTGCTGTGACTGCGGCTCATCAGAACCCACGTGGCTTTCAACCAACTTGGGTATTTTGACCTGTATA GAATGTTCCGGAATCCATAGGGAAATGGGGGTTCATATTTCTCGCATTCAGTCTTTGGAACTAGACAAAT TAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTAGGAAACAATAGTTTTAATGATATTATGGAAGCAAA TTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAAGTGATATGACCGTGCGTAAAGAGTACATCACTGCA AAGTATGTCGACCACCGCTTCTCCAGGAAGACCTGTGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTTG AGGCCATCAAGTCCAGGGACTTACTTGCATTAATCCAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGCC ACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAGCCCTTCATCTTGCCGTCAGGACCGCAGACCAGACA TCTCTCCATCTGGTTGATTTCCTTGTACAAAACTGTGGGAACCTGGATAAGCAAACGTCTGTGGGGAATA CAGTTCTGCACTATTGCAGCATGTACGGCAAGCCGGAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCAC CGTGGACATCGTTAACCAGAATGGAGAAACTGCCTTGGATATAGCAAAGAGACTCAAAGCTACCCAGTGT GAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAACCCTCATGTCCACGTAGAGTATGAGTGGAATC TTCGACAGGACGAGATGGATGAGAGCGATGACGATCTGGATGACAAGCCAAGCCCGATCAAAAAGGAACG CTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCAGCATCTCGCCACAGGACAAGCTGGCACTGCCAGGG TTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTACGGAGCCTTCACCAACCAGATCTTCGCCTCTACGA GCACAGATTTGCCCACATCACCCACCAGTGAGGCTCCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTAA TGATGTGGGCCCATTATCTTCAAGTAAGACGGCCAACAAGTTTGAGGGGCTGTCTCAGCAAGCAAGCACC AGTTCTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCCTAAACTACCTCAGAAAGTGGCACTAAGGAAGA CGGAGACCAGCCATCATCTCTCCCTCGACAGAACCAACATCCCACCTGAGACTTTTCAGAAATCATCACA GTTGACAGAGTTACCCCAAAAGCCACCACTTGGAGAGCTGCCCCCGAAGCCTGTGGAACTGGCCCCCAAG CCCCAAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCTACCCCCTAAGCCCCAATTAGGTGACCTGCCCC CCAAGCCACAGCTCTCAGACTTACCTCCCAAGCCACAGATGAAGGACCTGCCCCCTAAGCCGCAGCTGGG GGATCTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAGCCAAGGTGCAGCCACCCTCAGAGGTCACACAG AGGTCACACACCGGGGATCTGTCTCCAAATGTACAGTCCAGAGATGCCATCCAGAAGCAAGCATCTGAAG ACTCCAACGACCTCACACCCACGCTGCCAGAGACACCCGTACCACTGCCGAGAAAAATCAATACGGGGAA AAATAAAGTGAGGCGGGTGAAGACCATTTATGACTGCCAGGCAGATAATGACGACGAACTCACATTTATT GAGGGGGAAGTGATCATTGTCACCGGGGAAGAGGACCAGGAGTGGTGGATCGGGCATATCGAAGGACAGC CTGAAAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCACATCCTGTCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231570 representing NM_001276467 Red=Cloning site Green=Tags(s) MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTA LQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLS HNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKE RRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQD EEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKS DGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYI AWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCI ECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITA KYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQT SLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQC EDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPG FSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGNDVGPLSSSKTANKFEGLSQQAST SSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPK PQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQ RSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFI EGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276467
ORF Size	3270 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276467.1, NP_001263396.1
RefSeq Size	6110 bp
RefSeq ORF	3273 bp
Locus ID	13196
UniProt ID	Q9QWY8
MW	122.1 kDa
Gene Summary	May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.[UniProtKB/Swiss-Prot Function]

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