Ciita (NM_001243760) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C2t; C2ta; EG669998; Gm9475; Mhc2ta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231628 representing NM_001243760 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCACTTCCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAGCCCAGGCAGGTGC GGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCTCTCACGGGAATACCACTGTGCCTTGCTGCATGA GCCTGATGGTGATGCCCTGGCCCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTGACT TTCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAGGGGCACCAGCTACAGGGACCATG GAGACCATAGTCTGTGTGCCACCATGGATCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAG TGATGCCGACCCCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGAGGAGGAGATCGAA CTCAGCTCAGAGCCAGACACAGATACCATCAACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAAC TGGATGAAGAGACCCGGGAGGCCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCA GCTCGAGGGCCTGAGCAAGGACCTCTTCAAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATAGAG ATCCCTGTAGAAGCAGGACAGAAGCCTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGC ACAGGAAGCTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCACTGGGCACATCCAGAT CTTCACCACTCTGCCCCAGGGACTCTGGCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTAATC TACCACGGTGAGATGCCCCAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCCCCG AGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCCCGA ACCTGCGCTGACCTCCCGTGTAAATGAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAG TCTTCCATCAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCCTACAGGACAAATACA AGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAGAGG CAGCAACAAGAGCCAGGAAAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGTGGT CTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGCTGG GCAAGGCTGGCCAGGGAAAGAGCCACTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCT ACAATATGACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGACACCTACCACCTGCGG GATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTGAGGC AGCCAGACCGTGTTCTGCTCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCACGG ACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGGAAG CTACTGCGAGGCTGCACACTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGG CAGATGCCATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACATGAGGCACTACTTTGA GAACTCAGGGACAGCGGGGAACCAAGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGC TATAGTCACAGCCCTGTTGTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCACAG AGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAGTCC TCCCGGAGCCTTAGTCGAGCTGGCCAAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAA GAAACCCGGTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGATGCAGCAGACCCTGG AGACCACGGAGGCTCAACTGGCCTTCTCCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCTGGC ACAGTGCAATGAAATCAAAGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGACCC TATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACTGCC TGGGAGCTCTGGTGGAGCCTGCAGTGGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCT GAAGCGCCTGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCACTGTGCCCACGAGACA CAGCAACCTGGGATATGGGAGCATGTTGCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACCCGGC TCACACCCCCAGATGTGTATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCT TCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGG GCCTCCTTGAGTGATACAATGGCATTATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGG CGGCAGAGGAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGTGGAAGACCTGGATCG TCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCGGGAC CTTAAGAAGCTAGAGTTTGCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGATCC TTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAAGGG TGTGTCGAAGCTCTCAGCCACCTTCCCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAAC ATCACTGATGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGTCCCTCCTAAGGCTGA GCTTGTACAATAACTGCATCTGTGACAAAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGGTGTC CCTGCGTGTGATGGATGTCCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGCCTT CAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAACACC TGCAGCAGCTGGATGCCAGGATCAGTCTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231628 representing NM_001243760 Red=Cloning site Green=Tags(s) MNHFQAILAQVQTLLSSQKPRQVRALLDGLLEEELLSREYHCALLHEPDGDALARKISLTLLEKGDLDLT FLSWVCNSLQAPTVERGTSYRDHGDHSLCATMDLGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEEIE LSSEPDTDTINCDQFSKLLQDMELDEETREAYANIAELDQYVFQDTQLEGLSKDLFKHIGAEEGFGENIE IPVEAGQKPQKRRFPEEHAMDSKHRKLVPTSRTSLNYLDLPTGHIQIFTTLPQGLWQISGAGTGLSSVLI YHGEMPQVNQVLPSSSLSIPSLPESPDRPGSTSPFTPSAADLPSMPEPALTSRVNETEDTSPSPCQEGPE SSIKLPKWPEAVERFQHSLQDKYKALPQSPRGPLVAVELVRARLERGSNKSQERELATPDWTERQLAHGG LAEVLQVVSDCRRPGETQVVAVLGKAGQGKSHWARTVSHTWACGQLLQYDFVFYVPCHCLDRPGDTYHLR DLLCPPSLQPLAMDDEVLDYIVRQPDRVLLILDAFEELEAQDGLLHGPCGSLSPEPCSLRGLLAGIFQRK LLRGCTLLLTARPRGRLAQSLSKADAIFEVPSFSTKQAKTYMRHYFENSGTAGNQDKALGLLEGQPLLCS YSHSPVVCRAVCQLSKALLEQGTEAQLPCTLTGLYVSLLGPAAQNSPPGALVELAKLAWELGRRHQSTLQ ETRFSSVEVKTWAVTQGLMQQTLETTEAQLAFSSFLLQCFLGAVWLAQCNEIKDKELPQYLALTPRKKRP YDNWLEGVPRFLAGLVFQPRAHCLGALVEPAVAAVADRKQKVLTRYLKRLKLGTLRAGRLLELLHCAHET QQPGIWEHVAHQLPGHLSFLGTRLTPPDVYVLGRALETASQDFSLDLRQTGVEPSGLGNLVGLSCVTSFR ASLSDTMALWESLQQQGEAQLLQAAEEKFTIEPFKAKSPKDVEDLDRLVQTQRLRNPSEDAAKDLPAIRD LKKLEFALGPILGPQAFPTLAKILPAFSSLQHLDLDSLSENKIGDKGVSKLSATFPQLKALETLNLSQNN ITDVGACKLAEALPALAKSLLRLSLYNNCICDKGAKSLAQVLPDMVSLRVMDVQFNKFTAAGAQQLASSL QKCPQVETLAMWTPTIPFGVQEHLQQLDARISLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243760
ORF Size	3462 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243760.2, NP_001230689.1
RefSeq Size	5435 bp
RefSeq ORF	3465 bp
Locus ID	12265
MW	127.9 kDa
Gene Summary	This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

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