LCMT1 (NM_016309) Human Tagged ORF Clone
CAT＃: RC200018
LCMT1 (Myc-DDK-tagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1
ORF Plasmid: tGFP
"NM_016309" in other vectors (6)
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CNY 3656.00
CNY 5320.00
Product images
CNY 300.00
CNY 6840.00
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-68; LCMT; PPMT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200018 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGACGCAGACGACG AGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCAGTAAGCATTGGCTACTGGCA TGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAGAGGAAAGCCCCTGAAATCAACAGAGGATAT TTTGCTCGAGTCCATGGTGTCAGTCAGCTTATAAAGGCATTTCTACGGAAGACAGAATGTCATTGTCAAA TTGTCAACCTTGGGGCAGGCATGGATACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAA ATATTTTGAGGTTGACTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTA TCCAGCCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTGGATTCAAAGA GATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAGAAGCTAAAGAAATGTAACAT GAATACACAATTGCCAACACTCCTGATAGCTGAATGTGTGCTGGTTTACATGACTCCAGAGCAGTCCGCA AACCTCCTGAAGTGGGCAGCCAACAGTTTTGAGAGAGCCATGTTCATAAACTACGAACAGGTGAACATGG GTGATCGGTTTGGGCAGATCATGATTGAAAACCTGCGGAGACGCCAGTGTGACCTGGCGGGAGTGGAGAC CTGCAAGTCATTAGAGTCACAGAAAGAACGGCTCCTGTCGAATGGGTGGGAAACAGCATCGGCCGTCGAC ATGATGGAGTTGTACAACAGGTTACCTCGAGCTGAAGTGAGCAGGATAGAATCACTTGAATTCCTGGATG AAATGGAGCTGCTGGAGCAGCTCATGCGGCATTACTGCCTTTGCTGGGCAACCAAAGGAGGAAATGAGCT TGGGCTGAAGGAGATAACTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200018 protein sequence
Red=Cloning site Green=Tags(s) MATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGY FARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPL SSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSA NLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVD MMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_016309 |
| ORF Size | 1002 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016309.3 |
| RefSeq Size | 1374 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 51451 |
| UniProt ID | Q9UIC8 |
| Domains | LCM |
| Protein Pathways | Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism |
| MW | 38.4 kDa |
| Gene Summary | LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008] |
| Shipping | Ambient |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200018L1 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, Myc-DDK-tagged |
CNY 6056.00 |
|
| RC200018L2 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC200018L3 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC200018L4 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG200018 | LCMT1 (tGFP-tagged) - Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1 |
CNY 5256.00 |
|
| SC114308 | LCMT1 (untagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1 |
CNY 3656.00 |
