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KIF22 (NM_007317) Human Tagged ORF Clone
CAT＃: RC200397
KIF22 (Myc-DDK-tagged)-Human kinesin family member 22 (KIF22)
ORF Plasmid: tGFP
"NM_007317" in other vectors (6)
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CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A-328A3.2; KID; KNSL4; OBP; OBP-1; OBP-2; SEMDJL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200397 representing NM_007317
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGGGCGGCTCGACGCAGCAGAGGCGACGCGAGATGGCGGCAGCTTCAGCGGCGGCGATCTCAG GAGCTGGTCGCTGTCGGCTAAGCAAGATTGGAGCTACTCGTCGTCCACCTCCAGCTCGCGTAAGGGTGGC TGTGCGACTGCGGCCATTTGTGGATGGAACAGCGGGAGCAAGTGATCCCCCCTGTGTGCGGGGCATGGAC AGCTGCTCTCTAGAGATTGCTAACTGGAGGAACCACCAGGAGACTCTCAAATACCAGTTTGATGCCTTCT ATGGGGAGAGGAGTACTCAGCAGGACATCTATGCAGGTTCAGTGCAGCCCATCCTAAGGCACTTGCTGGA AGGGCAGAATGCCAGTGTGCTTGCCTATGGACCCACAGGAGCTGGGAAGACGCACACAATGCTGGGCAGC CCAGAGCAACCTGGGGTGATCCCGCGGGCTCTCATGGACCTCCTGCAGCTCACAAGGGAGGAGGGTGCCG AGGGCCGGCCATGGGCCCTTTCTGTCACCATGTCTTACCTAGAGATCTACCAGGAGAAGGTATTAGACCT CCTGGACCCTGCTTCGGGAGACCTGGTAATCCGAGAAGACTGCCGGGGGAATATCCTGATTCCGGGTCTC TCCCAGAAGCCCATCAGTAGCTTTGCTGATTTTGAGCGGCACTTCCTGCCAGCCAGTCGAAATCGGACTG TAGGAGCCACCCGGCTCAACCAGCGCTCCTCCCGCAGTCATGCTGTGCTCCTGGTCAAGGTGGACCAGCG GGAACGTTTGGCCCCATTTCGCCAGCGAGAGGGAAAACTCTACCTGATTGACTTGGCTGGGTCAGAGGAC AACCGGCGCACAGGCAACAAGGGCCTTCGGCTAAAAGAGAGTGGAGCCATCAACACCTCCCTGTTTGTCC TGGGCAAAGTGGTAGATGCGCTGAATCAGGGCCTCCCTCGTGTACCTTATCGGGACAGCAAGCTCACTCG CCTATTGCAGGACTCTCTGGGTGGCTCAGCCCACAGTATCCTTATTGCCAACATTGCCCCTGAGAGACGC TTCTACCTAGACACAGTCTCCGCACTCAACTTTGCTGCCAGGTCCAAGGAGGTGATCAATCGGCCTTTTA CCAATGAGAGCCTGCAGCCTCATGCCTTGGGACCTGTTAAGCTGTCTCAGAAAGAATTGCTTGGTCCACC AGAGGCAAAGAGAGCCCGAGGCCCTGAGGAAGAGGAGATTGGGAGCCCTGAGCCCATGGCAGCTCCAGCC TCTGCCTCCCAGAAACTCAGCCCCCTACAGAAGCTAAGCAGCATGGACCCGGCCATGCTGGAGCGCCTCC TCAGCTTGGACCGTCTGCTTGCCTCCCAGGGGAGCCAGGGGGCCCCTCTGTTGAGTACCCCAAAGCGAGA GCGGATGGTGCTAATGAAGACAGTAGAAGAGAAGGACCTAGAGATTGAGAGGCTTAAGACGAAGCAAAAA GAACTGGAGGCCAAGATGTTGGCCCAGAAGGCTGAGGAAAAGGAGAACCATTGTCCCACAATGCTCCGGC CCCTTTCACATCGCACAGTCACAGGGGCAAAGCCCCTGAAAAAGGCTGTGGTGATGCCCCTACAGCTAAT TCAGGAGCAGGCAGCATCCCCAAATGCCGAGATCCACATCCTGAAGAATAAAGGCCGGAAGAGAAAGCTG GAGTCCCTGGATGCCCTAGAGCCTGAGGAGAAGGCTGAGGACTGCTGGGAGCTACAGATCAGCCCGGAGC TACTGGCTCATGGGCGCCAAAAAATACTGGATCTGCTGAACGAAGGCTCAGCCCGAGATCTCCGCAGTCT TCAGCGCATTGGCCCGAAGAAGGCCCAGCTAATCGTGGGCTGGCGGGAGCTCCACGGCCCCTTCAGCCAG GTGGAGGACCTGGAACGCGTGGAGGGCATAACGGGGAAACAGATGGAGTCCTTCCTGAAGGCAAACATCC TGGGTCTCGCCGCCGGCCAGCGCTGTGGCGCCTCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC200397 representing NM_007317
Red=Cloning site Green=Tags(s) MAAGGSTQQRRREMAAASAAAISGAGRCRLSKIGATRRPPPARVRVAVRLRPFVDGTAGASDPPCVRGMD SCSLEIANWRNHQETLKYQFDAFYGERSTQQDIYAGSVQPILRHLLEGQNASVLAYGPTGAGKTHTMLGS PEQPGVIPRALMDLLQLTREEGAEGRPWALSVTMSYLEIYQEKVLDLLDPASGDLVIREDCRGNILIPGL SQKPISSFADFERHFLPASRNRTVGATRLNQRSSRSHAVLLVKVDQRERLAPFRQREGKLYLIDLAGSED NRRTGNKGLRLKESGAINTSLFVLGKVVDALNQGLPRVPYRDSKLTRLLQDSLGGSAHSILIANIAPERR FYLDTVSALNFAARSKEVINRPFTNESLQPHALGPVKLSQKELLGPPEAKRARGPEEEEIGSPEPMAAPA SASQKLSPLQKLSSMDPAMLERLLSLDRLLASQGSQGAPLLSTPKRERMVLMKTVEEKDLEIERLKTKQK ELEAKMLAQKAEEKENHCPTMLRPLSHRTVTGAKPLKKAVVMPLQLIQEQAASPNAEIHILKNKGRKRKL ESLDALEPEEKAEDCWELQISPELLAHGRQKILDLLNEGSARDLRSLQRIGPKKAQLIVGWRELHGPFSQ VEDLERVEGITGKQMESFLKANILGLAAGQRCGAS SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007317 |
| ORF Size | 1995 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007317.1, NP_015556.1 |
| RefSeq Size | 2097 bp |
| RefSeq ORF | 1998 bp |
| Locus ID | 3835 |
| UniProt ID | Q14807 |
| Domains | kinesin, HhH1 |
| Protein Families | Druggable Genome |
| MW | 73.1 kDa |
| Gene Summary | The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200397L1 | Lenti ORF clone of Human kinesin family member 22 (KIF22), Myc-DDK-tagged |
CNY 9,824.00 |
|
| RC200397L2 | Lenti ORF clone of Human kinesin family member 22 (KIF22), mGFP tagged |
CNY 5,890.00 |
|
| RC200397L3 | Lenti ORF clone of Human kinesin family member 22 (KIF22), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200397L4 | Lenti ORF clone of Human kinesin family member 22 (KIF22), mGFP tagged |
CNY 5,890.00 |
|
| RG200397 | KIF22 (tGFP-tagged) - Human kinesin family member 22 (KIF22) |
CNY 9,024.00 |
|
| SC319437 | KIF22 (untagged)-Human kinesin family member 22 (KIF22) |
CNY 7,440.00 |
