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FZR1 (NM_016263) Human Tagged ORF Clone
CAT＃: RC201413
FZR1 (Myc-DDK-tagged)-Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2
ORF Plasmid: tGFP
"NM_016263" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201413 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGGACTATGAGCGGCGCCTGCTTCGCCAGATCGTCATCCAGAATGAGAACACGATGCCACGCG TCACAGAGATGCGGCGGACCCTGACGCCTGCCAGCTCCCCAGTGTCCTCGCCCAGCAAGCACGGAGACCG CTTCATCCCCTCCAGAGCCGGAGCCAACTGGAGCGTGAACTTCCACAGGATTAACGAGAATGAGAAGTCT CCCAGTCAGAACCGGAAAGCCAAGGACGCCACCTCAGACAACGGCAAAGACGGCCTGGCCTACTCTGCCC TGCTCAAGAATGAGCTGCTGGGTGCCGGCATCGAGAAGGTGCAGGACCCGCAGACTGAGGACCGCAGGCT GCAGCCCTCCACGCCTGAGAAGAAGGGTCTGTTCACGTATTCCCTTAGCACCAAGCGCTCCAGCCCCGAT GACGGCAACGATGTGTCTCCCTACTCCCTGTCTCCCGTCAGCAACAAGAGCCAGAAGCTGCTCCGGTCCC CCCGGAAACCCACCCGCAAGATCTCCAAGATCCCCTTCAAGGTGCTGGACGCGCCCGAGCTGCAGGACGA CTTCTACCTCAATCTGGTGGACTGGTCGTCCCTCAATGTGCTCAGCGTGGGGCTAGGCACCTGCGTGTAC CTGTGGAGTGCCTGTACCAGCCAGGTGACGCGGCTCTGTGACCTCTCAGTGGAAGGGGACTCAGTGACCT CCGTGGGCTGGTCTGAGCGGGGGAACCTGGTGGCGGTGGGCACACACAAGGGCTTCGTGCAGATCTGGGA CGCAGCCGCAGGGAAGAAGCTGTCCATGTTGGAGGGCCACACGGCACGCGTCGGGGCGCTGGCCTGGAAT GCTGAGCAGCTGTCGTCCGGGAGCCGCGACCGCATGATCCTGCAGAGGGACATCCGCACCCCGCCACTGC AGTCGGAGCGGCGGCTGCAGGGCCACCGGCAGGAGGTGTGCGGGCTCAAGTGGTCCACAGACCACCAGCT CCTCGCCTCGGGGGGCAACGACAACAAGCTGCTGGTCTGGAATCACTCGAGCCTGAGCCCCGTGCAGCAG TACACGGAGCACCTGGCGGCCGTGAAGGCCATCGCCTGGTCCCCACATCAGCACGGGCTGCTGGCCTCGG GGGGCGGCACAGCTGACCGCTGTATCCGCTTCTGGAACACGCTGACAGGACAACCACTGCAGTGTATCGA CACGGGCTCCCAAGTGTGCAATCTGGCCTGGTCCAAGCACGCCAACGAGCTGGTGAGCACGCACGGCTAC TCACAGAACCAGATCCTTGTCTGGAAGTACCCCTCCCTGACCCAGGTGGCCAAGCTGACCGGGCACTCCT ACCGCGTGCTGTACCTGGCAATGTCCCCTGATGGGGAGGCCATCGTCACTGGTGCTGGAGACGAGACCCT GAGGTTCTGGAACGTCTTTAGCAAAACCCGTTCGACAAAGGAGTCTGTGTCTGTGCTCAACCTCTTCACC AGGATCCGG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC201413 protein sequence
Red=Cloning site Green=Tags(s) MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPASSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKS PSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPD DGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVY LWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWN AEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQ YTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGY SQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLFT RIR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016263 |
| ORF Size | 1479 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016263.4 |
| RefSeq Size | 3615 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 51343 |
| UniProt ID | Q9UM11 |
| Domains | WD40 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| MW | 54.8 kDa |
| Gene Summary | Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:25349192).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201413L1 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201413L2 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC201413L3 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201413L4 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201413 | FZR1 (tGFP-tagged) - Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2 |
CNY 4,370.00 |
|
| SC123643 | FZR1 (untagged)-Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2 |
CNY 2,950.00 |
