MAT1A (NM_000429) Human Tagged ORF Clone
CAT＃: RC203765
MAT1A (Myc-DDK-tagged)-Human methionine adenosyltransferase I, alpha (MAT1A)
ORF Plasmid: tGFP
"NM_000429" in other vectors (4)
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CNY 3656.00
CNY 3990.00
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CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MAT; MATA1; SAMS; SAMS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203765 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGACCGGTGGATGGCTTGTGTGACCACTCTCTAAGTGAAGGAGTCTTCATGTTCACATCGGAGT CTGTGGGAGAGGGACACCCGGATAAGATCTGTGACCAGATCAGTGATGCAGTGCTGGATGCCCATCTCAA GCAAGACCCCAATGCCAAGGTGGCCTGTGAGACAGTGTGCAAGACCGGCATGGTGCTGCTGTGTGGTGAG ATCACCTCAATGGCCATGGTGGACTACCAGCGGGTGGTGAGGGACACCATCAAGCACATCGGCTACGATG ACTCAGCCAAGGGCTTTGACTTCAAGACTTGCAACGTGCTGGTGGCTTTGGAGCAGCAATCCCCAGATAT TGCCCAGTGCGTCCATCTGGACAGAAATGAGGAGGATGTGGGGGCAGGAGATCAGGGTTTGATGTTCGGC TATGCCACCGACGAGACAGAGGAGTGCATGCCCCTCACCATCATCCTTGCTCACAAGCTCAACGCCCGGA TGGCAGACCTCAGGCGCTCCGGCCTCCTCCCCTGGCTGCGGCCTGACTCTAAGACTCAGGTGACAGTTCA GTACATGCAGGACAATGGCGCAGTCATCCCTGTGCGCATCCACACCATCGTCATCTCTGTGCAGCACAAC GAAGACATCACGCTGGAGGAGATGCGCAGGGCCCTGAAGGAGCAAGTCATCAGGGCCGTGGTGCCGGCCA AGTACCTGGACGAAGACACCGTCTACCACCTGCAGCCCAGTGGGCGGTTTGTCATCGGAGGTCCCCAGGG GGATGCGGGTGTCACTGGCCGTAAGATTATTGTGGACACCTATGGCGGCTGGGGGGCTCATGGTGGTGGG GCCTTCTCTGGGAAGGACTACACCAAGGTGGACCGCTCAGCCGCTTATGCTGCCCGCTGGGTGGCCAAGT CTCTGGTGAAAGCAGGGCTCTGCCGGAGAGTGCTTGTCCAGGTTTCCTATGCCATTGGTGTGGCCGAGCC GCTGTCCATTTCCATCTTCACCTACGGAACCTCTCAGAAGACAGAGCGAGAGCTGCTGGATGTGGTGCAT AAGAACTTCGACCTCCGGCCGGGCGTCATTGTCAGGGATTTGGACTTGAAGAAGCCCATCTACCAGAAGA CAGCATGCTACGGCCATTTCGGAAGAAGCGAGTTCCCATGGGAGGTTCCCAGGAAGCTTGTATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203765 protein sequence
Red=Cloning site Green=Tags(s) MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGE ITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFG YATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHN EDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGG AFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVH KNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_000429 |
| ORF Size | 1185 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000429.3 |
| RefSeq Size | 3419 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 4143 |
| UniProt ID | Q00266 |
| Domains | S-AdoMet_synt |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism |
| MW | 43.6 kDa |
| Gene Summary | This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008] |
| Shipping | Ambient |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203765L3 | Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC203765L4 | Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), mGFP tagged |
CNY 5890.00 |
|
| RG203765 | MAT1A (tGFP-tagged) - Human methionine adenosyltransferase I, alpha (MAT1A) |
CNY 5256.00 |
|
| SC119881 | MAT1A (untagged)-Human methionine adenosyltransferase I, alpha (MAT1A) |
CNY 3656.00 |
