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TIFA (NM_052864) Human Tagged ORF Clone
CAT＃: RC204357
TIFA (Myc-DDK-tagged)-Human TRAF-interacting protein with forkhead-associated domain (TIFA)
ORF Plasmid: tGFP
"NM_052864" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | T2BP; T6BP; TIFAA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204357 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGTTTTGAAGATGCTGACACAGAAGAGACAGTAACTTGTCTCCAGATGACGGTTTACCATCCTG GCCAGTTGCAGTGTGGAATATTTCAGTCAATAAGTTTTAACAGAGAGAAACTCCCTTCCAGCGAAGTGGT GAAATTTGGCCGAAATTCCAACATCTGTCATTATACTTTTCAGGACAAACAGGTTTCCCGAGTTCAGTTT TCTCTGCAGCTGTTTAAAAAATTCAACAGCTCAGTTCTCTCCTTTGAAATAAAAAATATGAGTAAAAAGA CCAATCTGATCGTGGACAGCAGAGAGCTGGGCTACCTAAATAAAATGGACCTGCCATACAGGTGCATGGT CAGATTCGGAGAGTATCAGTTTCTGATGGAGAAGGAAGATGGCGAGTCATTGGAATTTTTTGAGACTCAA TTTATTTTATCTCCAAGATCACTCTTGCAAGAAAACAACTGGCCACCACACAGGCCCATACCGGAGTATG GCACTTACTCGCTCTGCTCCTCCCAAAGCAGTTCTCCGACAGAAATGGATGAAAATGAGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204357 protein sequence
Red=Cloning site Green=Tags(s) MTSFEDADTEETVTCLQMTVYHPGQLQCGIFQSISFNREKLPSSEVVKFGRNSNICHYTFQDKQVSRVQF SLQLFKKFNSSVLSFEIKNMSKKTNLIVDSRELGYLNKMDLPYRCMVRFGEYQFLMEKEDGESLEFFETQ FILSPRSLLQENNWPPHRPIPEYGTYSLCSSQSSSPTEMDENES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_052864 |
| ORF Size | 552 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_052864.3 |
| RefSeq Size | 3090 bp |
| RefSeq ORF | 555 bp |
| Locus ID | 92610 |
| UniProt ID | Q96CG3 |
| MW | 21.4 kDa |
| Gene Summary | This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response. [provided by RefSeq, Jun 2018] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TIFA has dual functions in Helicobacter pylori‐induced classical and alternative NF‐κB pathways
,null,
EMBO Reports
,PubMed ID 34328245
[TIFA]
|
|
Helicobacter pylori modulates host cell responses by CagT4SS-dependent translocation of an intermediate metabolite of LPS inner core heptose biosynthesis
,Stein, SC;Faber, E;Bats, SH;Murillo, T;Speidel, Y;Coombs, N;Josenhans, C;,
PLoS Pathog.
,PubMed ID 28715499
[TIFA]
|
|
ALPK1 And TIFA Dependent Innate Immune Response Triggered By The Helicobacter Pylori Type IV Secretion System
,Zimmermann, S;Pfannkuch, L;Al-Zeer, M;Bartfeld, S;Koch, M;Liu, J;Rechner, C;Soerensen, M;Sokolova, O;Zamyatina, A;Kosma, P;Maeurer, AP;Glowinski, F;Pleissner, K;Schmid, M;Brinkmann, V;Naumann, M;Rother, M;Machuy, N;Meyer, TF;,
bioRxiv
[TIFA]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204357L3 | Lenti ORF clone of Human TRAF-interacting protein with forkhead-associated domain (TIFA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204357L4 | Lenti ORF clone of Human TRAF-interacting protein with forkhead-associated domain (TIFA), mGFP tagged |
CNY 5,890.00 |
|
| RG204357 | TIFA (tGFP-tagged) - Human TRAF-interacting protein with forkhead-associated domain (TIFA) |
CNY 4,000.00 |
|
| SC107567 | TIFA (untagged)-Human TRAF-interacting protein with forkhead-associated domain (TIFA) |
CNY 2,400.00 |
