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PARN (NM_002582) Human Tagged ORF Clone
CAT＃: RC207220
PARN (Myc-DDK-tagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1
ORF Plasmid: tGFP
"NM_002582" in other vectors (6)
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CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DAN; DKCB6; PFBMFT4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207220 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCCGACT TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTT TGACACTCCAGAAGAGAGGTATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTATTTCAGTTTGGC CTTTGCACTTTTAAGTATGACTACACAGATTCAAAGTATATAACGAAGTCATTTAACTTCTATGTTTTCC CGAAACCCTTCAATAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTAGC AAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAAGA CAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTATGTATCTC CTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAGAA AATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAAGA AAACTAATTTATCAGACTTTGAGCTGGAAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTGAAA AGAAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAACA TGCCAAAGAACAGGAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATTCG GGAAAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTACTGCCCTC TGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGACTCTTGGATACTAAATT GATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACATCCCTTGCGGAATTGGAAAAGCGGTTA AAAGAGACACCTTTCAACCCTCCTAAAGTTGAAAGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTCTG AACAACTCCACGAGGCAGGCTACGATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTACCT AGGTTCTTTTCTCAGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTAAC AAGTTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAGCCTAAAC GTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTTTACCAGCTTTTCAGTGC CTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCATTTGTTTCCCTTAGCCAGCCCGAGCAA GTAAAGATTGCTGTCAATACCAGCAAATATGCAGAAAGCTATCGGATCCAAACCTATGCTGAATATATGG GGAGAAAACAGGAAGAGAAGCAGATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGCAA ACGGTTAAACCCCCAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAGCT CCCAGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGAGTGTCAG GGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTCTCAGAGGGAAGGAAAAA GGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCAGGAAGCATCTCGAAGAACAGCCCTGCC ACACTCTTTGAAGTTCCTGACACATGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207220 protein sequence
Red=Cloning site Green=Tags(s) MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFG LCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEER QLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQR KLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANS GKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRL KETPFNPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSFLSPPKIHVSARSKLIEPFFN KLFLMRVMDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQ VKIAVNTSKYAESYRIQTYAEYMGRKQEEKQIKRKWTEDSWKEADSKRLNPQCIPYTLQNHYYRNNSFTA PSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEPLSEGRKKAKKLKRMKKELSPAGSISKNSPA TLFEVPDTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002582 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002582.4 |
| RefSeq Size | 3083 bp |
| RefSeq ORF | 1920 bp |
| Locus ID | 5073 |
| UniProt ID | O95453 |
| Domains | R3H, CAF1 |
| Protein Families | Transcription Factors |
| Protein Pathways | RNA degradation |
| MW | 73.5 kDa |
| Gene Summary | The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A feedback mechanism between PLD and deadenylase PARN for the shortening of eukaryotic poly(A) mRNA tails that is deregulated in cancer cells
,Miller, TE;Gomez-Cambronero, J;,
Biol Open
,PubMed ID 28011629
[PARN]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207220L1 | Lenti ORF clone of Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207220L2 | Lenti ORF clone of Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC207220L3 | Lenti ORF clone of Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207220L4 | Lenti ORF clone of Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG207220 | PARN (tGFP-tagged) - Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1 |
CNY 5,040.00 |
|
| SC118566 | PARN (untagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1 |
CNY 2,950.00 |
