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ADAMTS5 (NM_007038) Human Tagged ORF Clone
CAT＃: RC210834
- TrueORF®
ADAMTS5 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5)
ORF Plasmid: tGFP
"NM_007038" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADAM-TS 5; ADAM-TS5; ADAM-TS 11; ADAMTS-5; ADAMTS-11; ADAMTS11; ADMP-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210834 representing NM_007038.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGCTCGGGTGGGCGTCCCTGCTGCTGTGCGCGTTCCGCCTGCCCCTGGCCGCGGTCGGCCCCGCC GCGACACCTGCCCAGGATAAAGCCGGGCAGCCTCCGACTGCTGCAGCAGCCGCCCAGCCCCGCCGGCGG CAGGGGGAGGAGGTGCAGGAGCGAGCCGAGCCTCCCGGCCACCCGCACCCCCTGGCGCAGCGGCGCAGG AGCAAGGGGCTGGTGCAGAACATCGACCAACTCTACTCCGGCGGCGGCAAGGTGGGCTACCTCGTCTAC GCGGGCGGCCGGAGGTTCCTCTTGGACCTGGAGCGAGATGGTTCGGTGGGCATTGCTGGCTTCGTGCCC GCAGGAGGCGGGACGAGTGCGCCCTGGCGCCACCGGAGCCACTGCTTCTATCGGGGCACAGTGGACGCT AGTCCCCGCTCTCTGGCTGTCTTTGACCTCTGTGGGGGTCTCGACGGCTTCTTCGCGGTCAAGCACGCG CGCTACACCCTAAAGCCACTGCTGCGCGGACCCTGGGCGGAGGAAGAAAAGGGGCGCGTGTACGGGGAT GGGTCCGCACGGATCCTGCACGTCTACACCCGCGAGGGCTTCAGCTTCGAGGCCCTGCCGCCGCGCGCC AGCTGCGAAACCCCCGCGTCCACACCGGAGGCCCACGAGCATGCTCCGGCGCACAGCAACCCGAGCGGA CGCGCAGCACTGGCCTCGCAGCTCTTGGACCAGTCCGCTCTCTCGCCCGCTGGGGGCTCAGGACCGCAG ACGTGGTGGCGGCGGCGGCGCCGCTCCATCTCCCGGGCCCGCCAGGTGGAGCTGCTTCTGGTGGCTGAC GCGTCCATGGCGCGGTTGTATGGCCGGGGCCTGCAGCATTACCTGCTGACCCTGGCCTCCATCGCCAAT AGGCTGTACAGCCATGCTAGCATCGAGAACCACATCCGCCTGGCCGTGGTGAAGGTGGTGGTGCTAGGC GACAAGGACAAGAGCCTGGAAGTGAGCAAGAACGCTGCCACCACACTCAAGAACTTTTGCAAGTGGCAG CACCAACACAACCAGCTGGGAGATGACCATGAGGAGCACTACGATGCAGCTATCCTGTTTACTCGGGAG GATTTATGTGGGCATCATTCATGTGACACCCTGGGAATGGCAGACGTTGGGACCATATGTTCTCCAGAG CGCAGCTGTGCTGTGATTGAAGACGATGGCCTCCACGCAGCCTTCACTGTGGCTCACGAAATCGGACAT TTACTTGGCCTCTCCCATGACGATTCCAAATTCTGTGAAGAGACCTTTGGTTCCACAGAAGATAAGCGC TTAATGTCTTCCATCCTTACCAGCATTGATGCATCTAAGCCCTGGTCCAAATGCACTTCAGCCACCATC ACAGAATTCCTGGATGATGGCCATGGTAACTGTTTGCTGGACCTACCACGAAAGCAGATCCTGGGCCCC GAAGAACTCCCAGGACAGACCTACGATGCCACCCAGCAGTGCAACCTGACATTCGGGCCTGAGTACTCC GTGTGTCCCGGCATGGATGTCTGTGCTCGCCTGTGGTGTGCTGTGGTACGCCAGGGCCAGATGGTCTGT CTGACCAAGAAGCTGCCTGCGGTGGAAGGGACGCCTTGTGGAAAGGGGAGAATCTGCCTGCAGGGCAAA TGTGTGGACAAAACCAAGAAAAAATATTATTCAACGTCAAGCCATGGCAACTGGGGATCTTGGGGATCC TGGGGCCAGTGTTCTCGCTCATGTGGAGGAGGAGTGCAGTTTGCCTATCGTCACTGTAATAACCCTGCT CCCAGAAACAACGGACGCTACTGCACAGGGAAGAGGGCCATCTACCGCTCCTGCAGTCTCATGCCCTGC CCACCCAATGGTAAATCATTTCGTCATGAACAGTGTGAGGCCAAAAATGGCTATCAGTCTGATGCAAAA GGAGTCAAAACTTTTGTGGAATGGGTTCCCAAATATGCAGGTGTCCTGCCAGCGGATGTGTGCAAGCTG ACCTGCAGAGCCAAGGGCACTGGCTACTATGTGGTATTTTCTCCAAAGGTGACCGATGGCACTGAATGT AGGCCGTACAGTAATTCCGTCTGCGTCCGGGGGAAGTGTGTGAGAACTGGCTGTGACGGCATCATTGGC TCAAAGCTGCAGTATGACAAGTGCGGAGTATGTGGAGGAGACAACTCCAGCTGTACAAAGATTGTTGGA ACCTTTAATAAGAAAAGTAAGGGTTACACTGACGTGGTGAGGATTCCTGAAGGGGCAACCCACATAAAA GTTCGACAGTTCAAAGCCAAAGACCAGACTAGATTCACTGCCTATTTAGCCCTGAAAAAGAAAAACGGT GAGTACCTTATCAATGGAAAGTACATGATCTCCACTTCAGAGACTATCATTGACATCAATGGAACAGTC ATGAACTATAGCGGTTGGAGCCACAGGGATGACTTCCTGCATGGCATGGGCTACTCTGCCACGAAGGAA ATTCTAATAGTGCAGATTCTTGCAACAGACCCCACTAAACCATTAGATGTCCGTTATAGCTTTTTTGTT CCCAAGAAGTCCACTCCAAAAGTAAACTCTGTCACTAGTCATGGCAGCAATAAAGTGGGATCACACACT TCGCAGCCGCAGTGGGTCACGGGCCCATGGCTCGCCTGCTCTAGGACCTGTGACACAGGTTGGCACACC AGAACGGTGCAGTGCCAGGATGGAAACCGGAAGTTAGCAAAAGGATGTCCTCTCTCCCAAAGGCCTTCT GCGTTTAAGCAATGCTTGTTGAAGAAATGT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by RC210834
Blue=ORF Red=Cloning site Green=Tag(s) MLLGWASLLLCAFRLPLAAVGPAATPAQDKAGQPPTAAAAAQPRRRQGEEVQERAEPPGHPHPLAQRRR SKGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDLERDGSVGIAGFVPAGGGTSAPWRHRSHCFYRGTVDA SPRSLAVFDLCGGLDGFFAVKHARYTLKPLLRGPWAEEEKGRVYGDGSARILHVYTREGFSFEALPPRA SCETPASTPEAHEHAPAHSNPSGRAALASQLLDQSALSPAGGSGPQTWWRRRRRSISRARQVELLLVAD ASMARLYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLGDKDKSLEVSKNAATTLKNFCKWQ HQHNQLGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPERSCAVIEDDGLHAAFTVAHEIGH LLGLSHDDSKFCEETFGSTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGHGNCLLDLPRKQILGP EELPGQTYDATQQCNLTFGPEYSVCPGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRICLQGK CVDKTKKKYYSTSSHGNWGSWGSWGQCSRSCGGGVQFAYRHCNNPAPRNNGRYCTGKRAIYRSCSLMPC PPNGKSFRHEQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSPKVTDGTEC RPYSNSVCVRGKCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIVGTFNKKSKGYTDVVRIPEGATHIK VRQFKAKDQTRFTAYLALKKKNGEYLINGKYMISTSETIIDINGTVMNYSGWSHRDDFLHGMGYSATKE ILIVQILATDPTKPLDVRYSFFVPKKSTPKVNSVTSHGSNKVGSHTSQPQWVTGPWLACSRTCDTGWHT RTVQCQDGNRKLAKGCPLSQRPSAFKQCLLKKC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007038 |
| ORF Size | 2790 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007038.2, NP_008969.1 |
| RefSeq Size | 9663 bp |
| RefSeq ORF | 2793 bp |
| Locus ID | 11096 |
| UniProt ID | Q9UNA0 |
| Domains | tsp_1, Reprolysin, ACR |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 101.7 kDa |
| Gene Summary | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and functions as an aggrecanase that cleaves aggrecan, a major proteoglycan of cartilage, and may mediate cartilage destruction in osteoarthritis. [provided by RefSeq, Feb 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells
,null,
Oncotarget
,PubMed ID 28099917
[ADAMTS5]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210834L1 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210834L2 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5), mGFP tagged |
CNY 5,890.00 |
|
| RC210834L3 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210834L4 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5), mGFP tagged |
CNY 5,890.00 |
|
| RG210834 | ADAMTS5 (tGFP-tagged) - Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5) |
CNY 4,370.00 |
|
| SC115752 | ADAMTS5 (untagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5) |
CNY 6,816.00 |
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