Collagen IV (COL4A4) (NM_000092) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ATS2; BFH; CA44
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC211841 representing NM_000092 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTCTCTGCACATAGTACTAATGAGGTGCTCCTTCAGATTGACCAAGTCCTTGGCCACAGGTCCCT GGTCACTTATACTCATTCTCTTTTCTGTACAATATGTATATGGGAGTGGAAAGAAATACATTGGTCCTTG TGGAGGAAGAGATTGCTCTGTTTGCCACTGTGTTCCTGAAAAGGGGTCTCGGGGTCCACCAGGACCACCA GGGCCACAGGGTCCAATTGGACCCCTGGGAGCCCCAGGACCCATTGGGCTTTCAGGAGAGAAAGGAATGA GAGGGGACCGCGGCCCTCCTGGAGCAGCAGGGGACAAAGGAGATAAGGGTCCAACTGGTGTTCCTGGATT TCCAGGTTTAGATGGCATACCTGGGCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTATGAGTGGC CACAATGGCTCAAGAGGTGACCCAGGGTTTCCAGGAGGAAGAGGAGCTCTTGGCCCAGGAGGCCCCCTAG GCCATCCTGGGGAAAAGGGAGAAAAAGGAAATTCAGTGTTCATTTTAGGTGCCGTTAAAGGTATTCAGGG AGACAGAGGGGACCCAGGACTGCCTGGCTTACCAGGATCTTGGGGTGCAGGAGGACCGGCAGGTCCCACA GGATATCCTGGAGAGCCAGGGTTAGTGGGACCTCCGGGCCAACCAGGGCGTCCAGGTTTGAAGGGAAATC CCGGTGTGGGAGTAAAGGGGCAAATGGGAGACCCGGGTGAGGTTGGTCAGCAAGGTTCTCCTGGACCCAC CCTGTTGGTAGAGCCACCTGACTTTTGTCTCTATAAAGGAGAAAAGGGTATAAAAGGAATTCCTGGAATG GTTGGACTGCCAGGACCACCAGGACGCAAGGGAGAATCTGGTATTGGGGCAAAAGGAGAAAAAGGTATTC CTGGATTTCCAGGGCCTCGGGGGGATCCTGGTTCCTATGGATCTCCAGGTTTTCCAGGATTAAAGGGAGA ACTAGGACTGGTTGGAGATCCTGGGCTATTTGGATTAATTGGCCCAAAGGGGGATCCTGGAAATCGAGGG CACCCAGGACCACCAGGTGTTTTGGTGACTCCACCTCTTCCACTCAAAGGCCCACCAGGGGACCCAGGGT TCCCTGGCCGCTATGGAGAAACAGGGGATGTTGGACCACCTGGTCCCCCAGGTCTCTTGGGCAGACCAGG GGAAGCCTGTGCAGGCATGATAGGACCCCCTGGGCCACAAGGATTTCCTGGTCTTCCTGGGCTTCCAGGA GAAGCTGGTATTCCTGGGAGACCTGATTCTGCTCCAGGAAAACCAGGGAAGCCAGGATCACCTGGCTTGC CTGGAGCACCAGGCCTGCAGGGCCTCCCAGGATCAAGTGTGATATACTGTAGTGTTGGGAACCCCGGACC ACAAGGAATAAAAGGCAAAGTTGGTCCCCCAGGAGGAAGAGGCCCAAAAGGAGAAAAAGGAAATGAAGGA CTCTGTGCCTGTGAGCCTGGACCCATGGGCCCCCCTGGCCCTCCAGGACTTCCTGGGAGGCAGGGGAGTA AGGGAGACTTGGGGCTCCCTGGCTGGCTTGGAACAAAAGGTGACCCAGGACCTCCTGGTGCTGAAGGACC TCCAGGGCTACCAGGAAAGCATGGTGCCTCTGGACCACCTGGCAACAAAGGGGCGAAGGGTGACATGGTT GTATCAAGAGTTAAAGGGCACAAAGGAGAAAGAGGTCCTGATGGGCCCCCAGGATTTCCAGGGCAGCCAG GATCACATGGTCGGGATGGACATGCTGGAGAAAAAGGGGATCCAGGACCTCCAGGGGATCATGAAGATGC GACCCCAGGTGGTAAAGGATTTCCTGGACCTCTGGGCCCCCCAGGCAAAGCAGGACCTGTGGGGCCCCCA GGACTGGGATTTCCTGGTCCACCAGGAGAGCGAGGCCACCCAGGAGTTCCAGGCCACCCAGGTGTGAGGG GCCCTGATGGCTTGAAGGGTCAGAAAGGTGACACAATTTCTTGCAACGTAACCTACCCTGGGAGGCATGG CCCTCCAGGTTTTGATGGACCTCCAGGTCCGAAGGGATTTCCAGGTCCCCAAGGTGCCCCTGGGCTGAGT GGTTCAGATGGGCATAAAGGCAGACCTGGCACACCAGGAACAGCGGAAATACCAGGTCCACCTGGTTTTC GTGGTGACATGGGAGATCCGGGTTTTGGAGGTGAAAAGGGGTCCTCCCCTGTTGGGCCCCCAGGCCCTCC CGGCTCACCAGGAGTGAATGGTCAGAAAGGAATCCCGGGAGACCCTGCATTTGGTCACCTGGGACCCCCG GGAAAGAGGGGTCTTTCAGGAGTGCCAGGGATAAAAGGACCCAGAGGTGATCCGGGATGTCCAGGGGCTG AAGGGCCAGCTGGCATTCCTGGATTCCTAGGTCTCAAAGGTCCCAAAGGCAGAGAGGGACATGCTGGGTT TCCAGGTGTCCCAGGTCCACCTGGCCATTCCTGTGAAAGAGGTGCTCCAGGGATACCAGGGCAACCGGGA CTCCCTGGGTATCCAGGTAGCCCAGGTGCTCCAGGTGGGAAAGGACAGCCGGGAGATGTGGGGCCTCCCG GGCCAGCTGGAATGAAAGGCCTCCCCGGACTCCCAGGACGGCCTGGGGCACATGGTCCCCCAGGCCTCCC AGGAATCCCAGGTCCCTTTGGAGATGATGGGCTACCTGGTCCTCCAGGTCCAAAGGGACCCCGGGGGCTG CCTGGTTTCCCAGGTTTTCCCGGAGAAAGAGGAAAGCCTGGTGCAGAGGGATGTCCTGGCGCAAAGGGAG AACCTGGAGAGAAGGGCATGTCTGGCCTTCCTGGAGACCGGGGACTGAGAGGGGCCAAAGGAGCCATAGG ACCTCCCGGAGATGAAGGAGAAATGGCTATCATTTCACAAAAGGGAACACCTGGGGAACCTGGACCTCCT GGAGATGATGGATTCCCAGGAGAAAGAGGTGATAAAGGAACTCCCGGGATGCAAGGGAGAAGAGGAGAGC CGGGAAGATACGGACCACCTGGATTTCACAGAGGGGAACCTGGTGAGAAAGGTCAGCCAGGGCCTCCTGG ACCCCCAGGCCCTCCAGGCTCAACTGGTCTAAGAGGGTTCATTGGTTTTCCAGGACTTCCAGGTGACCAG GGTGAGCCAGGTTCTCCAGGTCCCCCTGGATTTTCAGGAATTGATGGAGCAAGAGGACCTAAAGGAAACA AAGGTGACCCTGCCAGTCACTTTGGTCCACCTGGTCCAAAGGGTGAGCCAGGTAGCCCTGGATGTCCAGG GCATTTTGGAGCATCCGGAGAGCAGGGCTTGCCTGGTATTCAAGGGCCCAGAGGATCACCTGGAAGGCCA GGGCCACCTGGCTCCTCTGGACCACCAGGGTGCCCAGGTGATCACGGGATGCCTGGGCTGAGGGGACAGC CAGGAGAAATGGGAGACCCTGGGCCAAGAGGCCTCCAGGGGGATCCAGGGATACCAGGTCCTCCGGGAAT AAAAGGTCCCTCCGGATCACCTGGCCTGAACGGCTTGCATGGATTGAAAGGTCAGAAAGGAACTAAAGGT GCTTCAGGTTTGCATGATGTGGGGCCACCTGGTCCAGTGGGAATACCTGGGCTAAAAGGGGAGAGAGGAG ACCCTGGGAGCCCAGGAATCTCTCCTCCAGGTCCTCGTGGAAAGAAAGGTCCCCCAGGACCCCCAGGGAG TTCAGGACCACCTGGTCCTGCAGGTGCCACAGGAAGAGCTCCTAAGGACATTCCTGACCCGGGTCCACCT GGAGATCAGGGACCTCCTGGTCCTGATGGCCCAAGAGGAGCACCTGGGCCTCCAGGCCTCCCTGGGAGTG TTGACCTTCTGAGAGGGGAGCCAGGTGACTGTGGTCTACCAGGGCCACCAGGTCCCCCTGGCCCACCAGG CCCTCCAGGATACAAAGGCTTTCCAGGATGTGATGGAAAAGATGGCCAGAAAGGACCAGTGGGATTCCCG GGACCGCAGGGACCACATGGATTTCCTGGGCCACCTGGAGAGAAGGGTTTACCTGGACCTCCAGGGAGAA AAGGGCCCACTGGTCTTCCGGGTCCCAGAGGTGAACCGGGGCCACCTGCAGATGTGGATGACTGTCCCCG AATCCCAGGCCTTCCTGGGGCGCCAGGCATGAGAGGACCAGAAGGAGCCATGGGGCTCCCTGGAATGAGA GGCCCCTCAGGACCAGGGTGCAAAGGAGAGCCTGGGCTGGATGGCAGGAGGGGTGTGGATGGCGTCCCTG GGTCTCCTGGGCCTCCCGGACGTAAAGGTGACACAGGAGAAGACGGCTACCCTGGAGGACCAGGGCCTCC TGGTCCCATTGGGGATCCTGGGCCCAAAGGGTTTGGCCCTGGATACCTCGGTGGCTTCCTCCTGGTTCTC CACAGTCAGACGGACCAGGAGCCCACCTGCCCCCTGGGCATGCCCAGGCTCTGGACTGGGTATAGTCTGT TATACCTGGAAGGGCAAGAGAAAGCTCACAATCAAGACCTTGGTCTGGCAGGGTCTTGCCTTCCCGTATT TAGCACGCTGCCCTTTGCCTACTGCAACATCCACCAGGTGTGCCACTATGCCCAGAGAAACGACAGATCC TACTGGCTGGCCAGCGCTGCGCCCCTCCCCATGATGCCACTCTCTGAAGAGGCGATCCGCCCCTATGTCA GCCGCTGTGCGGTATGCGAGGCCCCGGCCCAGGCGGTGGCGGTGCACAGCCAGGACCAGTCCATCCCCCC ATGTCCGCAGACCTGGAGGAGCCTCTGGATCGGGTATTCATTCCTGATGCACACAGGAGCTGGGGACCAA GGAGGAGGGCAGGCCCTTATGTCACCTGGCAGCTGCCTGGAAGATTTCAGAGCAGCACCATTCCTTGAAT GCCAGGGCCGGCAGGGAACTTGCCACTTTTTCGCAAATAAGTATAGCTTCTGGCTCACAACGGTGAAAGC AGACTTGCAGTTTTCCTCTGCTCCAGCACCAGACACCTTAAAAGAAAGCCAGGCCCAACGCCAGAAAATC AGCCGGTGCCAGGTCTGCGTGAAGTATAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC211841 representing NM_000092 Red=Cloning site Green=Tags(s) MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPP GPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSG HNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPT GYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGM VGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRG HPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPG EAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEG LCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMV VSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPP GLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLS GSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPP GKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPG LPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGL PGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPP GDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQ GEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRP GPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKG ASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPP GDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFP GPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMR GPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVL HSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRS YWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQ GGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKI SRCQVCVKYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_000092
ORF Size	5070 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000092.5
RefSeq Size	7843 bp
RefSeq ORF	5073 bp
Locus ID	1286
UniProt ID	P53420
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
MW	164.04 kDa
Gene Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]

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