Collagen IV (COL4A4) (NM_000092) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATS2; BFH; CA44
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC300009 representing NM_000092. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGTCTCTGCACATAGTACTAATGAGGTGCTCCTTCAGATTGACCAAGTCCTTGGCCACAGGTCCC TGGTCACTTATACTCATTCTCTTTTCTGTACAATATGTATATGGGAGTGGAAAGAAATACATTGGTCCT TGTGGAGGAAGAGATTGCTCTGTTTGCCACTGTGTTCCTGAAAAGGGGTCTCGGGGTCCACCAGGACCA CCAGGGCCACAGGGTCCAATTGGACCCCTGGGAGCCCCAGGACCCATTGGGCTTTCAGGAGAGAAAGGA ATGAGAGGGGACCGCGGCCCTCCTGGAGCAGCAGGGGACAAAGGAGATAAGGGTCCAACTGGTGTTCCT GGATTTCCAGGTTTAGATGGCATACCTGGGCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTATG AGTGGCCACAATGGCTCAAGAGGTGACCCAGGGTTTCCAGGAGGAAGAGGAGCTCTTGGCCCAGGAGGC CCCCTAGGCCATCCTGGGGAAAAGGGAGAAAAAGGAAATTCAGTGTTCATTTTAGGTGCCGTTAAAGGT ATTCAGGGAGACAGAGGGGACCCAGGACTGCCTGGCTTACCAGGATCTTGGGGTGCAGGAGGACCGGCA GGTCCCACAGGATATCCTGGAGAGCCAGGGTTAGTGGGACCTCCGGGCCAACCAGGGCGTCCAGGTTTG AAGGGAAATCCCGGTGTGGGAGTAAAGGGGCAAATGGGAGACCCGGGTGAGGTTGGTCAGCAAGGTTCT CCTGGACCCACCCTGTTGGTAGAGCCACCTGACTTTTGTCTCTATAAAGGAGAAAAGGGTATAAAAGGA ATTCCTGGAATGGTTGGACTGCCAGGACCACCAGGACGCAAGGGAGAATCTGGTATTGGGGCAAAAGGA GAAAAAGGTATTCCTGGATTTCCAGGGCCTCGGGGGGATCCTGGTTCCTATGGATCTCCAGGTTTTCCA GGATTAAAGGGAGAACTAGGACTGGTTGGAGATCCTGGGCTATTTGGATTAATTGGCCCAAAGGGGGAT CCTGGAAATCGAGGGCACCCAGGACCACCAGGTGTTTTGGTGACTCCACCTCTTCCACTCAAAGGCCCA CCAGGGGACCCAGGGTTCCCTGGCCGCTATGGAGAAACAGGGGATGTTGGACCACCTGGTCCCCCAGGT CTCTTGGGCAGACCAGGGGAAGCCTGTGCAGGCATGATAGGACCCCCTGGGCCACAAGGATTTCCTGGT CTTCCTGGGCTTCCAGGAGAAGCTGGTATTCCTGGGAGACCTGATTCTGCTCCAGGAAAACCAGGGAAG CCAGGATCACCTGGCTTGCCTGGAGCACCAGGCCTGCAGGGCCTCCCAGGATCAAGTGTGATATACTGT AGTGTTGGGAACCCCGGACCACAAGGAATAAAAGGCAAAGTTGGTCCCCCAGGAGGAAGAGGCCCAAAA GGAGAAAAAGGAAATGAAGGACTCTGTGCCTGTGAGCCTGGACCCATGGGCCCCCCTGGCCCTCCAGGA CTTCCTGGGAGGCAGGGGAGTAAGGGAGACTTGGGGCTCCCTGGCTGGCTTGGAACAAAAGGTGACCCA GGACCTCCTGGTGCTGAAGGACCTCCAGGGCTACCAGGAAAGCATGGTGCCTCTGGACCACCTGGCAAC AAAGGGGCGAAGGGTGACATGGTTGTATCAAGAGTTAAAGGGCACAAAGGAGAAAGAGGTCCTGATGGG CCCCCAGGATTTCCAGGGCAGCCAGGATCACATGGTCGGGATGGACATGCTGGAGAAAAAGGGGATCCA GGACCTCCAGGGGATCATGAAGATGCGACCCCAGGTGGTAAAGGATTTCCTGGACCTCTGGGCCCCCCA GGCAAAGCAGGACCTGTGGGGCCCCCAGGACTGGGATTTCCTGGTCCACCAGGAGAGCGAGGCCACCCA GGAGTTCCAGGCCACCCAGGTGTGAGGGGCCCTGATGGCTTGAAGGGTCAGAAAGGTGACACAATTTCT TGCAACGTAACCTACCCTGGGAGGCATGGCCCTCCAGGTTTTGATGGACCTCCAGGTCCGAAGGGATTT CCAGGTCCCCAAGGTGCCCCTGGGCTGAGTGGTTCAGATGGGCATAAAGGCAGACCTGGCACACCAGGA ACAGCGGAAATACCAGGTCCACCTGGTTTTCGTGGTGACATGGGAGATCCGGGTTTTGGAGGTGAAAAG GGGTCCTCCCCTGTTGGGCCCCCAGGCCCTCCCGGCTCACCAGGAGTGAATGGTCAGAAAGGAATCCCG GGAGACCCTGCATTTGGTCACCTGGGACCCCCGGGAAAGAGGGGTCTTTCAGGAGTGCCAGGGATAAAA GGACCCAGAGGTGATCCGGGATGTCCAGGGGCTGAAGGGCCAGCTGGCATTCCTGGATTCCTAGGTCTC AAAGGTCCCAAAGGCAGAGAGGGACATGCTGGGTTTCCAGGTGTCCCAGGTCCACCTGGCCATTCCTGT GAAAGAGGTGCTCCAGGGATACCAGGGCAACCGGGACTCCCTGGGTATCCAGGTAGCCCAGGTGCTCCA GGTGGGAAAGGACAGCCGGGAGATGTGGGGCCTCCCGGGCCAGCTGGAATGAAAGGCCTCCCCGGACTC CCAGGACGGCCTGGGGCACATGGTCCCCCAGGCCTCCCAGGAATCCCAGGTCCCTTTGGAGATGATGGG CTACCTGGTCCTCCAGGTCCAAAGGGACCCCGGGGGCTGCCTGGTTTCCCAGGTTTTCCCGGAGAAAGA GGAAAGCCTGGTGCAGAGGGATGTCCTGGCGCAAAGGGAGAACCTGGAGAGAAGGGCATGTCTGGCCTT CCTGGAGACCGGGGACTGAGAGGGGCCAAAGGAGCCATAGGACCTCCCGGAGATGAAGGAGAAATGGCT ATCATTTCACAAAAGGGAACACCTGGGGAACCTGGACCTCCTGGAGATGATGGATTCCCAGGAGAAAGA GGTGATAAAGGAACTCCCGGGATGCAAGGGAGAAGAGGAGAGCCGGGAAGATACGGACCACCTGGATTT CACAGAGGGGAACCTGGTGAGAAAGGTCAGCCAGGGCCTCCTGGACCCCCAGGCCCTCCAGGCTCAACT GGTCTAAGAGGGTTCATTGGTTTTCCAGGACTTCCAGGTGACCAGGGTGAGCCAGGTTCTCCAGGTCCC CCTGGATTTTCAGGAATTGATGGAGCAAGAGGACCTAAAGGAAACAAAGGTGACCCTGCCAGTCACTTT GGTCCACCTGGTCCAAAGGGTGAGCCAGGTAGCCCTGGATGTCCAGGGCATTTTGGAGCATCCGGAGAG CAGGGCTTGCCTGGTATTCAAGGGCCCAGAGGATCACCTGGAAGGCCAGGGCCACCTGGCTCCTCTGGA CCACCAGGGTGCCCAGGTGATCACGGGATGCCTGGGCTGAGGGGACAGCCAGGAGAAATGGGAGACCCT GGGCCAAGAGGCCTCCAGGGGGATCCAGGGATACCAGGTCCTCCGGGAATAAAAGGTCCCTCCGGATCA CCTGGCCTGAACGGCTTGCATGGATTGAAAGGTCAGAAAGGAACTAAAGGTGCTTCAGGTTTGCATGAT GTGGGGCCACCTGGTCCAGTGGGAATACCTGGGCTAAAAGGGGAGAGAGGAGACCCTGGGAGCCCAGGA ATCTCTCCTCCAGGTCCTCGTGGAAAGAAAGGTCCCCCAGGACCCCCAGGGAGTTCAGGACCACCTGGT CCTGCAGGTGCCACAGGAAGAGCTCCTAAGGACATTCCTGACCCGGGTCCACCTGGAGATCAGGGACCT CCTGGTCCTGATGGCCCAAGAGGAGCACCTGGGCCTCCAGGCCTCCCTGGGAGTGTTGACCTTCTGAGA GGGGAGCCAGGTGACTGTGGTCTACCAGGGCCACCAGGTCCCCCTGGCCCACCAGGCCCTCCAGGATAC AAAGGCTTTCCAGGATGTGATGGAAAAGATGGCCAGAAAGGACCAGTGGGATTCCCGGGACCGCAGGGA CCACATGGATTTCCTGGGCCACCTGGAGAGAAGGGTTTACCTGGACCTCCAGGGAGAAAAGGGCCCACT GGTCTTCCGGGTCCCAGAGGTGAACCGGGGCCACCTGCAGATGTGGATGACTGTCCCCGAATCCCAGGC CTTCCTGGGGCGCCAGGCATGAGAGGACCAGAAGGAGCCATGGGGCTCCCTGGAATGAGAGGCCCCTCA GGACCAGGGTGCAAAGGAGAGCCTGGGCTGGATGGCAGGAGGGGTGTGGATGGCGTCCCTGGGTCTCCT GGGCCTCCCGGACGTAAAGGTGACACAGGAGAAGACGGCTACCCTGGAGGACCAGGGCCTCCTGGTCCC ATTGGGGATCCTGGGCCCAAAGGGTTTGGCCCTGGATACCTCGGTGGCTTCCTCCTGGTTCTCCACAGT CAGACGGACCAGGAGCCCACCTGCCCCCTGGGCATGCCCAGGCTCTGGACTGGGTATAGTCTGTTATAC CTGGAAGGGCAAGAGAAAGCTCACAATCAAGACCTTGGTCTGGCAGGGTCTTGCCTTCCCGTATTTAGC ACGCTGCCCTTTGCCTACTGCAACATCCACCAGGTGTGCCACTATGCCCAGAGAAACGACAGATCCTAC TGGCTGGCCAGCGCTGCGCCCCTCCCCATGATGCCACTCTCTGAAGAGGCGATCCGCCCCTATGTCAGC CGCTGTGCGGTATGCGAGGCCCCGGCCCAGGCGGTGGCGGTGCACAGCCAGGACCAGTCCATCCCCCCA TGTCCGCAGACCTGGAGGAGCCTCTGGATCGGGTATTCATTCCTGATGCACACAGGAGCTGGGGACCAA GGAGGAGGGCAGGCCCTTATGTCACCTGGCAGCTGCCTGGAAGATTTCAGAGCAGCACCATTCCTTGAA TGCCAGGGCCGGCAGGGAACTTGCCACTTTTTCGCAAATAAGTATAGCTTCTGGCTCACAACGGTGAAA GCAGACTTGCAGTTTTCCTCTGCTCCAGCACCAGACACCTTAAAAGAAAGCCAGGCCCAACGCCAGAAA ATCAGCCGGTGCCAGGTCTGCGTGAAGTATAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_000092
Insert Size	5073 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000092.4
RefSeq Size	10341 bp
RefSeq ORF	5073 bp
Locus ID	1286
UniProt ID	P53420
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
MW	164 kDa
Gene Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]

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