HIC1 (NM_001098202) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	hic-1; ZBTB29; ZNF901
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC212163 representing NM_001098202 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTTTCCTGAAGCGGACATTTTACTTAAATCGGGAGAGTGTGCTGGGCAGACGATGCTGGACACGA TGGAGGCGCCCGGCCACTCCAGGCAGCTGCTGCTGCAGCTCAACAACCAGCGCACCAAGGGCTTCTTGTG CGACGTGATCATCGTGGTGCAGAACGCCCTCTTCCGCGCGCACAAGAACGTGCTGGCGGCCAGCAGCGCC TACCTCAAGTCCCTGGTGGTGCATGACAACCTGCTCAACCTGGACCATGACATGGTGAGCCCGGCCGTGT TCCGCCTGGTGCTGGACTTCATCTACACCGGCCGCCTGGCTGACGGCGCAGAGGCGGCTGCGGCCGCGGC CGTGGCCCCGGGGGCTGAGCCGAGCCTGGGCGCCGTGCTGGCCGCCGCCAGCTACCTGCAGATCCCCGAC CTCGTGGCGCTGTGCAAGAAACGCCTCAAGCGCCACGGCAAGTACTGCCACCTGCGGGGCGGCGGCGGCG GCGGCGGCGGCTACGCGCCCTATGGTCGGCCGGGCCGGGGCCTGCGGGCCGCCACGCCGGTCATCCAGGC CTGCTACCCGTCCCCAGTCGGGCCTCCGCCGCCGCCTGCCGCGGAGCCGCCCTCGGGCCCAGAGGCCGCG GTCAACACGCACTGCGCCGAGCTGTACGCGTCGGGACCCGGCCCGGCCGCCGCACTCTGTGCCTCGGAGC GCCGCTGCTCCCCTCTTTGTGGCCTGGACCTGTCCAAGAAGAGCCCGCCGGGCTCCGCGGCGCCAGAGCG GCCGCTGGCTGAGCGCGAGCTGCCCCCGCGCCCGGACAGCCCTCCCAGCGCCGGCCCCGCCGCCTACAAG GAGCCGCCTCTCGCCCTGCCGTCGCTGCCGCCGCTGCCCTTCCAGAAGCTGGAGGAGGCCGCACCGCCTT CCGACCCATTTCGCGGCGGCAGCGGCAGCCCGGGACCCGAGCCCCCCGGCCGCCCCGACGGGCCTAGTCT CCTCTATCGCTGGATGAAGCACGAGCCGGGCCTGGGTAGCTATGGCGACGAGCTGGGCCGGGAGCGCGGC TCCCCCAGCGAGCGCTGCGAAGAGCGTGGTGGGGACGCGGCCGTCTCGCCCGGGGGGCCCCCGCTCGGCC TGGCGCCGCCGCCGCGCTACCCTGGCAGCCTGGACGGGCCCGGCGCGGGCGGCGACGGCGACGACTACAA GAGCAGCAGCGAGGAGACCGGTAGCAGCGAGGACCCCAGCCCGCCTGGCGGCCACCTCGAGGGCTACCCA TGCCCGCACCTGGCCTATGGCGAGCCCGAGAGCTTCGGTGACAACCTGTACGTGTGCATTCCGTGCGGCA AGGGCTTCCCCAGCTCTGAGCAGCTGAACGCGCACGTGGAGGCTCACGTGGAGGAGGAGGAAGCGCTGTA CGGCAGGGCCGAGGCGGCCGAAGTGGCCGCTGGGGCCGCCGGCCTAGGGCCCCCTTTTGGAGGCGGCGGG GACAAGGTCGCCGGGGCTCCGGGTGGCCTGGGAGAGCTGCTGCGGCCCTACCGCTGCGCGTCGTGCGACA AGAGCTACAAGGACCCGGCCACGCTGCGGCAGCACGAGAAGACGCACTGGCTGACCCGGCCCTACCCATG CACCATCTGCGGGAAGAAGTTCACGCAGCGTGGGACCATGACGCGCCACATGCGCAGCCACCTGGGCCTC AAGCCCTTCGCGTGCGACGCGTGCGGCATGCGGTTCACGCGCCAGTACCGCCTCACGGAGCACATGCGCA TCCACTCGGGCGAGAAGCCCTACGAGTGCCAGGTGTGCGGCGGCAAGTTCGCACAGCAACGCAACCTCAT CAGCCACATGAAGATGCACGCCGTGGGGGGCGCGGCCGGCGCGGCCGGGGCGCTGGCGGGCTTGGGGGGG CTCCCCGGCGTCCCCGGCCCCGACGGCAAGGGCAAGCTCGACTTCCCCGAGGGCGTCTTTGCTGTGGCTC GCCTCACGGCCGAGCAGCTGAGCCTGAAGCAGCAGGACAAGGCGGCCGCGGCCGAGCTGCTGGCGCAGAC CACGCACTTCCTGCACGACCCCAAGGTGGCGCTGGAGAGCCTCTACCCGCTGGCCAAGTTCACGGCCGAG CTGGGCCTCAGCCCCGACAAGGCGGCCGAGGTGCTGAGCCAGGGCGCTCACCTGGCGGCCGGGCCCGACG GCCGGACCATCGACCGTTTCTCTCCCACC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC212163 representing NM_001098202 Red=Cloning site Green=Tags(s) MTFPEADILLKSGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSA YLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPD LVALCKKRLKRHGKYCHLRGGGGGGGGYAPYGRPGRGLRAATPVIQACYPSPVGPPPPPAAEPPSGPEAA VNTHCAELYASGPGPAAALCASERRCSPLCGLDLSKKSPPGSAAPERPLAERELPPRPDSPPSAGPAAYK EPPLALPSLPPLPFQKLEEAAPPSDPFRGGSGSPGPEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERG SPSERCEERGGDAAVSPGGPPLGLAPPPRYPGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYP CPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGG DKVAGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGL KPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGG LPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAE LGLSPDKAAEVLSQGAHLAAGPDGRTIDRFSPT SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001098202
ORF Size	2199 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001098202.1, NP_001091672.1
RefSeq Size	3071 bp
RefSeq ORF	2202 bp
Locus ID	3090
UniProt ID	Q14526
MW	76.5 kDa
Gene Summary	This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010]

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