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NEDD4 (NM_198400) Human Tagged ORF Clone
CAT＃: RC212738
NEDD4 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2
ORF Plasmid: tGFP
"NM_198400" in other vectors (6)
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CNY 8,680.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NEDD4-1; RPF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212738 representing NM_198400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAAAGCTTACGATTGCACTTTGCAGCCAGAAGAAGCAATACTTACCCTTTGTCAGAAACCTCCG GAGATGACTTGGATAGCCATGTTCACATGTGCTTCAAAAGACCAACACGGATTTCAACGTCTAACGTTGT TCAAATGAAGCTGACTCCCAGACAGACTGCACTAGCTCCGTTAATAAAGGAAAACGTTCAGTCTCAAGAA AGATCATCTGTTCCCTCATCTGAAAATGTTAATAAAAAGAGCAGCTGTCTACAGATTTCACTACAGCCAA CAAGGTACAGTGGATATCTTCAGTCTAGCAATGTCTTAGCTGATAGTGATGATGCTTCGTTTACTTGTAT CTTGAAGGATGGTATTTACAGTAGTGCTGTGGTCGATAATGAATTGAATGCTGTGAATGATGGTCACCTT GTAAGCAGTCCAGCCATTTGTAGTGGTAGCCTTAGTAACTTTTCAACCAGTGATAATGGGTCTTACAGCA GCAACGGTAGTGATTTTGGGTCATGTGCAAGTATCACAAGTGGAGGTTCATATACTAACAGTGTCATCAG TGACAGTAGTAGTTATACTTTTCCACCAAGTGATGATACTTTTTTGGGTGGAAACTTACCTTCTGACAGC ACCTCCAATAGAAGTGTGCCAAACAGGAATACTACTCCTTGTGAAATTTTTTCAAGAAGTACAAGTACAG ATCCTTTTGTCCAGGATGACTTGGAACATGGATTAGAGATTATGAAATTGCCAGTGAGCAGGAACACAAA AATTCCACTAAAACGTTACTCCTCCTTAGTCATTTTTCCTAGGAGTCCTTCAACTACCCGACCGACTTCT CCAACAAGTCTGTGTACTCTTCTGAGCAAAGGATCCTATCAAACTTCACACCAGTTTATTATTTCTCCTA GTGAAATTGCACATAATGAGGATGGCACTAGTGCTAAAGGATTTCTTTCAACAGCTGTCAATGGACTTCG GTTATCTAAAACAATTTGTACCCCCGGAGAAGTAAGAGACATACGGCCGCTTCACAGGAAGGGCTCGTTA CAGAAGAAAATTGTTCTTTCGAATAATACTCCCAGACAGACTGTCTGTGAAAAGTCATCTGAAGGATATT CTTGTGTTTCAGTGCATTTCACCCAACGAAAAGCAGCTACATTAGACTGTGAAACAACAAATGGTGATTG TAAACCAGAAATGTCAGAAATTAAGCTTAATTCTGATTCAGAGTATATTAAGCTCATGCATAGGACATCT GCATGTTTGCCATCCTCCCAAAATGTAGATTGTCAAATAAATATCAATGGAGAATTGGAAAGACCACATT CACAGATGAACAAAAACCATGGTATTTTACGAAGAAGTATTTCATTGGGAGGAGCTTATCCAAATATTTC CTGTCTATCCAGCCTTAAGCACAATTGTTCTAAAGGGGGACCATCTCAGTTACTCATAAAGTTTGCATCT GGAAATGAAGGTAAAGTGGATAATTTATCAAGAGACAGCAACAGAGATTGCACAAATGAACTGTCTAATT CTTGCAAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACAAGAACC TTCTCCTCTACCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAA TCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAATGGCAACATTC AACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGAAACAGAAAGTGTTGACAACCG AGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAACCAGGCCTTCCCA TCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCAGAAGAATTGAATGCCAGACTCACCA TTTTTGGAAATTCAGCCGTGAGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACA AGCCTATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCACCAGGT TGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAACGACTACTTGGA CAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGCCCTCA ATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCCATCTGAAATTGAGCAAGGATTCCTT CCTAAAGGCTGGGAAGTCCGGCATGCACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCA CCACCTGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATACTTCCAA TGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTACATAAAT CACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGCAGTGC CCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTTGAAGAAGCAGAATGACATTCC AAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTC AAGAGAGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGGGATTGGATTATGGAG GAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTTTGAATA TTCTGCTACGGACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACCTCTCT TACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGATGGTTTTTTCA TCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGA ATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTCAGGTTTATCATAGAT GAAGAACTTTTTGGACAGACACATCAACATGAGCTGAAAAATGGTGGATCAGAAATAGTTGTCACCAATA AGAACAAAAAGGAATATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAAATGGC TGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAATGAACTA GAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATG GCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGATGGATTCAGAAAAAAG AATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTGCCTATGAATGGATTTGCTGAACTATACGGT TCAAATGGACCACAGTCATTTACAGTTGAACAGTGGGGTACTCCTGAAAAGCTGCCAAGAGCTCATACCT GTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGATGGCAAT TGAAAACACCCAGGGCTTTGATGGAGTTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212738 representing NM_198400
Red=Cloning site Green=Tags(s) MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQE RSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHL VSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDS TSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTS PTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSL QKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTS ACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFAS GNEGKVDNLSRDSNRDCTNELSNSCKPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHE SRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFP SPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPG WEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFL PKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYIN HNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGV KRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLS YFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIID EELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENEL ELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYG SNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198400 |
| ORF Size | 3741 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198400.3 |
| RefSeq Size | 7024 bp |
| RefSeq ORF | 3744 bp |
| Locus ID | 4734 |
| UniProt ID | P46934 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| MW | 140.7 kDa |
| Gene Summary | This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212738L1 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged |
CNY 11,080.00 |
|
| RC212738L2 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged |
CNY 14,350.00 |
|
| RC212738L3 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged |
CNY 11,080.00 |
|
| RC212738L4 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged |
CNY 14,350.00 |
|
| RG212738 | NEDD4 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 |
CNY 13,680.00 |
|
| SC123396 | NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 |
CNY 8,688.00 |
