RIZ1 (PRDM2) (NM_012231) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HUMHOXY1; KMT8; KMT8A; MTB-ZF; RIZ; RIZ1; RIZ2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC214550 representing NM_012231 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCAGAACACTACTGAGCCTGTGGCGGCCACCGAGACCCTGGCTGAGGTACCCGAACATGTGCTGC GAGGACTTCCGGAGGAAGTGAGGCTTTTCCCTTCTGCTGTTGACAAGACCCGGATTGGTGTCTGGGCCAC TAAACCAATTTTAAAAGGCAAAAAATTTGGGCCATTTGTTGGTGATAAGAAAAAAAGATCTCAGGTTAAG AATAATGTATACATGTGGGAGGTGTATTACCCAAATTTGGGATGGATGTGCATTGATGCCACTGATCCAG AGAAGGGAAACTGGCTGCGATATGTGAATTGGGCTTGCTCAGGAGAAGAGCAAAATTTATTCCCACTGGA AATCAACAGAGCCATTTACTATAAAACTTTAAAGCCAATCGCGCCGGGCGAGGAGCTCCTGGTCTGGTAC AATGGGGAAGACAACCCTGAGATAGCAGCTGCGATTGAGGAAGAGCGAGCCAGCGCCCGGAGCAAGCGGA GCTCCCCCAAGAGCCGGAAAGGGAAGAAAAAATCCCAGGAAAATAAAAACAAAGGAAACAAAATCCAAGA CATACAACTGAAGACAAGTGAGCCAGATTTCACCTCTGCAAATATGAGAGATTCTGCAGAAGGTCCTAAA GAAGACGAAGAGAAGCCTTCAGCCTCAGCACTTGAGCAGCCGGCCACCCTCCAGGAGGTGGCCAGTCAGG AGGTGCCTCCAGAACTAGCAACCCCTGCCCCTGCCTGGGAGCCACAGCCAGAACCAGACGAGCGATTAGA AGCGGCAGCTTGTGAGGTGAATGATTTGGGGGAAGAGGAGGAGGAGGAAGAGGAGGAGGATGAAGAAGAA GAAGAAGATGATGATGATGATGAGTTGGAAGACGAGGGGGAAGAAGAAGCCAGCATGCCAAATGAAAATT CTGTGAAAGAGCCAGAAATACGGTGTGATGAGAAGCCAGAAGATTTATTAGAGGAACCAAAAACAACTTC AGAAGAAACTCTTGAAGACTGCTCAGAGGTAACACCTGCCATGCAAATCCCCAGAACTAAAGAAGAGGCC AATGGTGATGTATTTGAAACGTTTATGTTTCCGTGTCAACATTGTGAAAGGAAGTTTACAACCAAACAGG GGCTTGAGCGTCACATGCATATCCATATATCCACCGTCAATCATGCTTTCAAATGCAAGTACTGTGGGAA AGCCTTTGGCACACAGATTAACCGGCGGCGACATGAGCGGCGCCATGAAGCAGGGTTAAAGCGGAAACCC AGCCAAACACTACAGCCGTCAGAGGATCTGGCTGATGGCAAAGCATCTGGAGAAAACGTTGCTTCAAAAG ATGATTCGAGTCCTCCCAGTCTTGGGCCAGACTGTCTGATCATGAATTCAGAGAAGGCTTCCCAAGACAC AATAAATTCTTCTGTCGTAGAAGAGAATGGGGAAGTTAAAGAACTTCATCCGTGCAAATATTGTAAAAAG GTTTTTGGAACTCATACTAATATGAGACGGCATCAGCGTAGAGTTCACGAACGTCATCTGATTCCCAAAG GTGTACGGCGAAAAGGAGGCCTTGAAGAGCCCCAGCCTCCAGCAGAACAGGCCCAGGCCACCCAGAACGT GTATGTACCAAGCACAGAGCCGGAGGAGGAAGGGGAAGCAGATGATGTGTACATCATGGACATTTCTAGC AATATCTCTGAAAACTTAAATTACTATATTGATGGTAAAATTCAAACTAATAACAACACTAGTAACTGTG ATGTGATTGAGATGGAGTCTGCTTCGGCAGATTTGTATGGTATAAATTGTCTGCTCACTCCAGTTACAGT GGAAATTACTCAAAATATAAAGACCACACAGGTCCCTGTAACAGAAGATCTTCCTAAAGAGCCTTTGGGC AGCACAAATAGTGAGGCCAAGAAGCGGAGAACTGCGAGCCCACCTGCACTGCCCAAAATTAAGGCCGAAA CAGACTCTGACCCCATGGTCCCCTCTTGCTCTTTAAGTCTTCCTCTTAGCATATCAACAACAGAGGCAGT GTCTTTCCACAAAGAGAAAAGTGTTTATTTGTCATCAAAGCTCAAACAACTTCTTCAAACCCAAGATAAA CTAACTCCTGCAGGGATTTCAGCAACTGAAATAGCTAAATTAGGTCCTGTTTGTGTGTCTGCTCCTGCAT CAATGTTGCCTGTGACCTCAAGTAGGTTTAAGAGGCGGACCAGCTCTCCTCCCAGTTCTCCACAGCACAG TCCTGCCCTTCGAGACTTTGGAAAGCCAAGTGATGGGAAAGCAGCATGGACCGATGCCGGGCTGACTTCC AAAAAATCCAAATTAGAAAGTCACAGCGACTCACCAGCATGGAGTTTGTCTGGGAGAGATGAGAGAGAAA CTGTGAGCCCTCCATGCTTTGATGAATATAAAATGTCTAAAGAGTGGACAGCTAGTTCTGCTTTTAGCAG TGTGTGCAACCAGCAGCCACTGGATTTATCCAGCGGTGTCAAACAGAAGGCTGAGGGTACAGGCAAGACT CCAGTCCAGTGGGAATCTGTCTTAGATCTCAGTGTGCATAAAAAGCATTGTAGTGACTCTGAAGGCAAGG AATTCAAAGAAAGTCATTCAGTGCAGCCTACGTGTAGTGCTGTAAAGAAAAGGAAACCAACCACCTGCAT GCTGCAGAAGGTTCTTCTCAATGAATATAATGGCATCGATTTACCTGTAGAAAACCCTGCAGATGGGACC AGGAGCCCAAGTCCTTGTAAATCCCTAGAAGCTCAGCCAGATCCTGACCTCGGTCCGGGCTCTGGTTTCC CTGCCCCTACTGTTGAGTCCACACCTGATGTTTGTCCTTCATCACCTGCCCTGCAGACACCCTCCCTTTC ATCCGGTCAGCTGCCTCCTCTCTTGATCCCCACAGATCCCTCTTCCCCTCCACCCTGTCCCCCGGTATTA ACTGTTGCCACTCCGCCCCCTCCCCTCCTTCCTACCGTACCTCTTCCAGCCCCCTCTTCCAGTGCATCTC CACACCCATGCCCCTCTCCACTCTCAAATGCCACCGCACAGTCCCCACTTCCAATTCTGTCCCCAACAGT GTCCCCCTCTCCCTCTCCCATTCCTCCCGTGGAGCCCCTGATGTCTGCCGCCTCACCCGGGCCTCCAACA CTTTCTTCTTCCTCCTCTTCATCTTCCTCCTCCTCTTCGTTTTCTTCTTCATCTTCCTCCTCTTCTCCTT CTCCACCTCCTCTCTCCGCAATATCATCTGTTGTTTCCTCTGGTGATAATCTGGAGGCTTCTCTCCCCAT GATATCTTTCAAACAGGAGGAATTAGAGAATGAAGGTCTGAAACCCAGGGAAGAGCCCCAGTCTGCTGCT GAACAGGATGTTGTTGTTCAGGAAACATTCAACAAAAACTTTGTTTGCAACGTCTGTGAATCACCTTTTC TTTCCATTAAAGATCTAACCAAACATTTATCTATTCATGCTGAAGAATGGCCCTTCAAATGTGAATTTTG TGTGCAGCTTTTTAAGGATAAAACGGACTTGTCAGAACATCGCTTTTTGCTTCATGGAGTTGGGAATATC TTTGTGTGTTCTGTTTGTAAAAAAGAATTTGCTTTTTTGTGCAATTTGCAGCAGCACCAGCGAGATCTCC ACCCAGATAAGGTGTGCACACATCACGAGTTTGAAAGCGGGACTCTGAGGCCCCAGAACTTTACAGATCC CAGCAAGGCCCATGTAGAGCATATGCAGAGCTTGCCAGAAGATCCTTTAGAAACTTCTAAAGAAGAAGAG GAGTTAAATGATTCCTCTGAAGAGCTTTACACGACTATAAAAATAATGGCTTCTGGAATAAAGACAAAAG ATCCAGATGTTCGATTGGGCCTCAATCAGCATTACCCAAGCTTTAAACCACCTCCATTTCAGTACCATCA CCGTAACCCCATGGGGATTGGTGTGACAGCCACAAATTTCACTACACACAATATTCCACAGACTTTCACT ACCGCCATTCGCTGCACAAAGTGTGGAAAAGGTGTCGACAATATGCCGGAGTTGCACAAACATATCCTGG CTTGTGCTTCTGCAAGTGACAAGAAGAGGTACACGCCTAAGAAAAACCCAGTACCATTAAAACAAACTGT GCAACCCAAAAATGGCGTGGTGGTTTTAGATAACTCTGGGAAAAATGCCTTCCGACGAATGGGACAGCCC AAAAGGCTTAACTTTAGTGTTGAGCTCAGCAAAATGTCGTCGAATAAGCTCAAATTAAATGCATTGAAGA AAAAAAATCAGCTAGTACAGAAAGCAATTCTTCAGAAAAACAAATCTGCAAAGCAGAAGGCCGACTTGAA AAATGCTTGTGAGTCATCCTCTCACATCTGCCCTTACTGTAATCGAGAGTTCACTTACATTGGAAGCCTG AATAAACACGCCGCCTTCAGCTGTCCCAAAAAACCCCTTTCTCCTCCCAAAAAAAAAGTTTCTCATTCAT CTAAGAAAGGTGGACACTCATCACCTGCAAGTAGTGACAAAAACAGTAACAGCAACCACCGCAGACGGAC AGCGGATGCGGAGATTAAAATGCAAAGCATGCAGACTCCGTTGGGCAAGACCAGAGCCCGCAGCTCAGGC CCCACCCAAGTCCCACTTCCCTCCTCATCCTTCAGGTCCAAGCAGAACGTCAAGTTTGCAGCTTCGGTGA AATCCAAAAAACCAAGCTCCTCCTCTTTAAGGAACTCCAGCCCGATAAGAATGGCCAAAATAACTCATGT TGAGGGGAAAAAACCTAAAGCTGTGGCCAAGAATCATTCTGCTCAGCTTTCCAGCAAAACATCACGGAGC CTGCACGTGAGGGTACAGAAAAGCAAAGCTGTTTTACAAAGCAAATCCACCTTGGCGAGTAAGAAAAGAA CAGACCGGTTCAATATAAAATCTAGAGAGCGGAGTGGGGGGCCAGTCACCCGGAGCCTTCAGCTGGCAGC TGCTGCTGACTTGAGTGAGAACAAGAGAGAGGACGGCAGCGCCAAGCAGGAGCTGAAGGACTTCAGCTAC AGCCTCCGCTTGGCGTCCCGATGCTCTCCACCAGCGGCCCCGTACATCACCAGGCAGTATAGGAAGGTCA AAGCTCCGGCTGCAGCCCAGTTCCAGGGACCATTCTTCAAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC214550 representing NM_012231 Red=Cloning site Green=Tags(s) MNQNTTEPVAATETLAEVPEHVLRGLPEEVRLFPSAVDKTRIGVWATKPILKGKKFGPFVGDKKKRSQVK NNVYMWEVYYPNLGWMCIDATDPEKGNWLRYVNWACSGEEQNLFPLEINRAIYYKTLKPIAPGEELLVWY NGEDNPEIAAAIEEERASARSKRSSPKSRKGKKKSQENKNKGNKIQDIQLKTSEPDFTSANMRDSAEGPK EDEEKPSASALEQPATLQEVASQEVPPELATPAPAWEPQPEPDERLEAAACEVNDLGEEEEEEEEEDEEE EEDDDDDELEDEGEEEASMPNENSVKEPEIRCDEKPEDLLEEPKTTSEETLEDCSEVTPAMQIPRTKEEA NGDVFETFMFPCQHCERKFTTKQGLERHMHIHISTVNHAFKCKYCGKAFGTQINRRRHERRHEAGLKRKP SQTLQPSEDLADGKASGENVASKDDSSPPSLGPDCLIMNSEKASQDTINSSVVEENGEVKELHPCKYCKK VFGTHTNMRRHQRRVHERHLIPKGVRRKGGLEEPQPPAEQAQATQNVYVPSTEPEEEGEADDVYIMDISS NISENLNYYIDGKIQTNNNTSNCDVIEMESASADLYGINCLLTPVTVEITQNIKTTQVPVTEDLPKEPLG STNSEAKKRRTASPPALPKIKAETDSDPMVPSCSLSLPLSISTTEAVSFHKEKSVYLSSKLKQLLQTQDK LTPAGISATEIAKLGPVCVSAPASMLPVTSSRFKRRTSSPPSSPQHSPALRDFGKPSDGKAAWTDAGLTS KKSKLESHSDSPAWSLSGRDERETVSPPCFDEYKMSKEWTASSAFSSVCNQQPLDLSSGVKQKAEGTGKT PVQWESVLDLSVHKKHCSDSEGKEFKESHSVQPTCSAVKKRKPTTCMLQKVLLNEYNGIDLPVENPADGT RSPSPCKSLEAQPDPDLGPGSGFPAPTVESTPDVCPSSPALQTPSLSSGQLPPLLIPTDPSSPPPCPPVL TVATPPPPLLPTVPLPAPSSSASPHPCPSPLSNATAQSPLPILSPTVSPSPSPIPPVEPLMSAASPGPPT LSSSSSSSSSSSSFSSSSSSSSPSPPPLSAISSVVSSGDNLEASLPMISFKQEELENEGLKPREEPQSAA EQDVVVQETFNKNFVCNVCESPFLSIKDLTKHLSIHAEEWPFKCEFCVQLFKDKTDLSEHRFLLHGVGNI FVCSVCKKEFAFLCNLQQHQRDLHPDKVCTHHEFESGTLRPQNFTDPSKAHVEHMQSLPEDPLETSKEEE ELNDSSEELYTTIKIMASGIKTKDPDVRLGLNQHYPSFKPPPFQYHHRNPMGIGVTATNFTTHNIPQTFT TAIRCTKCGKGVDNMPELHKHILACASASDKKRYTPKKNPVPLKQTVQPKNGVVVLDNSGKNAFRRMGQP KRLNFSVELSKMSSNKLKLNALKKKNQLVQKAILQKNKSAKQKADLKNACESSSHICPYCNREFTYIGSL NKHAAFSCPKKPLSPPKKKVSHSSKKGGHSSPASSDKNSNSNHRRRTADAEIKMQSMQTPLGKTRARSSG PTQVPLPSSSFRSKQNVKFAASVKSKKPSSSSLRNSSPIRMAKITHVEGKKPKAVAKNHSAQLSSKTSRS LHVRVQKSKAVLQSKSTLASKKRTDRFNIKSRERSGGPVTRSLQLAAAADLSENKREDGSAKQELKDFSY SLRLASRCSPPAAPYITRQYRKVKAPAAAQFQGPFFKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012231
ORF Size	5154 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012231.3, NP_036363.2
RefSeq Size	7958 bp
RefSeq ORF	5157 bp
Locus ID	7799
UniProt ID	Q13029
Domains	SET, zf-C2H2
Protein Families	Druggable Genome
MW	188.7 kDa
Gene Summary	This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

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