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TMEM97 (NM_014573) Human Tagged ORF Clone
CAT＃: RC216927
TMEM97 (Myc-DDK-tagged)-Human transmembrane protein 97 (TMEM97)
ORF Plasmid: tGFP
"NM_014573" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MAC30 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216927 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCTCCGGCAACCAGGCGCTGCGTGGAGTGGCTGCTGGGCCTCTACTTCCTCAGCCACATCCCCA TCACCCTGTTCATGGACCTGCAGGCGGTGCTGCCGCGCGAGCTCTACCCAGTCGAGTTTAGAAACCTGCT GAAGTGGTATGCTAAGGAGTTCAAAGACCCACTGCTACAGGAGCCCCCAGCCTGGTTTAAGTCCTTTCTG TTTTGCGAGCTTGTGTTTCAGCTGCCTTTCTTTCCCATTGCAACGTATGCCTTCCTCAAAGGAAGCTGCA AGTGGATTCGAACTCCTGCAATCATCTACTCTGTTCACACCATGACAACCTTAATTCCGATACTCTCCAC ATTTCTGTTTGAGGATTTCTCCAAAGCCAGTGGTTTCAAGGGACAAAGACCTGAGACTTTGCATGAACGG TTAACCCTTGTGTCTGTCTATGCCCCCTACTTACTCATCCCATTCATACTTTTAATTTTCATGTTGCGGA GCCCCTACTACAAGTATGAAGAGAAAAGAAAAAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216927 protein sequence
Red=Cloning site Green=Tags(s) MGAPATRRCVEWLLGLYFLSHIPITLFMDLQAVLPRELYPVEFRNLLKWYAKEFKDPLLQEPPAWFKSFL FCELVFQLPFFPIATYAFLKGSCKWIRTPAIIYSVHTMTTLIPILSTFLFEDFSKASGFKGQRPETLHER LTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014573 |
| ORF Size | 528 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014573.3 |
| RefSeq Size | 2585 bp |
| RefSeq ORF | 531 bp |
| Locus ID | 27346 |
| UniProt ID | Q5BJF2 |
| Protein Families | Transmembrane |
| MW | 20.8 kDa |
| Gene Summary | TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels (Bartz et al., 2009 [PubMed 19583955]).[supplied by OMIM, Aug 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
NO1, a New Sigma 2 Receptor/TMEM97 Fluorescent Ligand, Downregulates SOCE and Promotes Apoptosis in the Triple Negative Breast Cancer Cell Lines
,Cantonero, C;Camello, PJ;Abate, C;Berardi, F;Salido, GM;Rosado, JA;Redondo, PC;,
Cancers (Basel) 2020
,PubMed ID 31973006
[TMEM97]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216927L1 | Lenti ORF clone of Human transmembrane protein 97 (TMEM97), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216927L2 | Lenti ORF clone of Human transmembrane protein 97 (TMEM97), mGFP tagged |
CNY 5,890.00 |
|
| RC216927L3 | Lenti ORF clone of Human transmembrane protein 97 (TMEM97), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216927L4 | Lenti ORF clone of Human transmembrane protein 97 (TMEM97), mGFP tagged |
CNY 4,800.00 |
|
| RG216927 | TMEM97 (tGFP-tagged) - Human transmembrane protein 97 (TMEM97) |
CNY 4,000.00 |
|
| SC107367 | TMEM97 (untagged)-Human transmembrane protein 97 (TMEM97) |
CNY 1,200.00 |
|
| SC317273 | TMEM97 (untagged)-Human transmembrane protein 97 (TMEM97) |
CNY 2,400.00 |
