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PHC3 (NM_024947) Human Tagged ORF Clone
CAT＃: RC217729
- TrueORF®
PHC3 (Myc-DDK-tagged)-Human polyhomeotic homolog 3 (Drosophila) (PHC3)
ORF Plasmid: tGFP
"NM_024947" in other vectors (7)
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CNY 10,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EDR3; HPH3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217729 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACTGAACCAAACCCGGGAACATCTTCTGTGTCAACAACAACCAGCAGTACCACCACCACCACCA TCACCACTTCCTCCTCTCGAATGCAGCAGCCACAGATCTCTGTCTACAGTGGTTCAGACCGACATGCTGT ACAGGTAATTCAACAGGCATTGCATCGGCCCCCCAGCTCAGCTGCTCAGTACCTTCAGCAAATGTATGCA GCCCAACAACAGCACTTGATGCTGCATACTGCAGCTCTTCAGCAGCAGCATTTAAGCAGCTCCCAGCTTC AGAGCCTTGCTGCTGTTCAGGCAAGTTTGTCCAGTGGAAGACCATCTACATCTCCCACAGGAAGTGTCAC ACAGCAGTCAAGTATGTCCCAAACGTCTATCAACCTCTCCACTTCTCCTACACCTGCACAGTTAATAAGC CGTTCCCAGGCTTCCAGTTCTACCAGCGGCAGTATTACCCAACAGACTATGTTACTAGGGAGTACTTCCC CTACCCTAACGGCAAGCCAAGCTCAAATGTATCTCCGAGCTCAAATGCTGATTTTCACACCCGCTACCAC TGTGGCTGCTGTACAGTCTGACATTCCTGTTGTCTCGTCGTCATCGTCATCTTCCTGTCAGTCTGCAGCT ACTCAGGTTCAGAATTTAACATTACGCAGCCAGAAGTTGGGTGTATTATCTAGCTCACAGAATGGTCCAC CAAAAAGCACTAGTCAAACTCAGTCATTGACAATTTGTCATAACAAAACAACAGTGACCAGTTCTAAAAT CAGCCAACGAGATCCTTCTCCAGAAAGTAATAAGAAAGGAGAGAGCCCAAGCCTGGAATCACGAAGCACA GCTGTCACCCGGACATCAAGTATTCACCAGTTAATAGCACCAGCTTCATATTCTCCAATTCAGCCTCATT CTCTAATAAAACATCAGCAGATTCCTCTTCATTCACCACCTTCCAAAGTTTCCCATCATCAGCTGATATT ACAACAGCAGCAACAGCAAATTCAGCCAATCACACTTCAGAATTCAACTCAAGACCCACCCCCATCCCAG CACTGTATACCACTCCAGAACCATGGCCTTCCTCCAGCTCCCAGTAATGCCCAGTCACAGCATTGTTCAC CGATTCAGAGTCATCCCTCTCCTTTAACAGTGTCTCCTAATCAGTCACAGTCAGCACAGCAGTCTGTAGT GGTGTCTCCTCCACCACCTCATTCACCAAGTCAGTCTCCTACTATAATTATTCATCCACAAGCACTTATT CAGCCACACCCTCTTGTGTCATCAGCTCTCCAGCCAGGGCCAAATTTGCAGCAGTCCACTGCTAATCAGG TGCAAGCTACAGCACAGTTGAATCTTCCATCCCATCTTCCACTTCCAGCTTCCCCTGTTGTACACATTGG CCCAGTTCAGCAGTCTGCCTTGGTATCCCCAGGCCAGCAGATTGTCTCTCCATCACACCAGCAATATTCA TCCCTGCAGTCCTCTCCAATCCCAATTGCAAGTCCTCCACAGATGTCGACATCTCCTCCAGCTCAGATTC CACCACTGCCCTTGCAGTCTATGCAGTCTTTACAAGTGCAGCCTGAAATTCTGTCCCAGGGCCAGGTTTT GGTGCAGAATGCTTTGGTGTCAGAAGAGGAACTTCCAGCTGCAGAAGCTTTGGTCCAGTTGCCATTTCAG ACTCTTCCTCCTCCACAGACTGTTGCGGTAAACCTACAAGTGCAACCACCAGCACCTGTTGATCCACCAG TGGTTTATCAGGTAGAAGATGTGTGTGAAGAAGAAATGCCAGAAGAGTCAGATGAATGTGTCCGGATGGA TAGAACCCCACCACCACCCACTTTGTCTCCAGCAGCTATAACAGTGGGGAGAGGAGAAGATTTGACTTCT GAACATCCTTTGTTAGAGCAAGTGGAATTACCTGCTGTGGCATCAGTCAGTGCTTCAGTAATTAAATCTC CATCAGATCCCTCACATGTTTCTGTTCCACCACCTCCATTGTTACTTCCAGCTGCCACCACAAGGAGTAA CAGTACATCTATGCACAGTAGCATTCCCAGTATAGAGAACAAACCTCCACAGGCTATTGTTAAACCACAG ATCCTAACCCATGTTATTGAAGGCTTTGTGATTCAGGAGGGATTGGAGCCATTTCCTGTGAGTCGTTCCT CTTTGCTAATAGAACAGCCTGTGAAAAAACGGCCTCTTTTGGATAATCAGGTGATAAATTCAGTGTGTGT TCAGCCAGAGCTACAGAATAATACAAAACATGCGGATAATTCATCTGACACAGAGATGGAAGACATGATT GCTGAAGAGACATTAGAAGAAATGGACAGTGAGTTGCTCAAGTGTGAATTCTGTGGGAAAATGGGATATG CTAATGAATTTTTGCGGTCAAAACGATTCTGCACTATGTCATGTGCCAAAAGGTACAATGTTAGCTGTTC TAAAAAATTTGCACTTAGTCGTTGGAATCGTAAGCCTGATAATCAAAGTCTTGGGCATCGTGGCCGTCGT CCAAGTGGCCCTGATGGGGCAGCGAGAGAACATATCCTTAGGCAGCTTCCAATTACTTATCCATCTGCAG AAGAAGACTTGGCTTCTCATGAAGATTCTGTGCCATCTGCTATGACAACTCGTCTGCGCAGGCAGAGCGA GCGGGAAAGAGAACGTGAGCTTCGGGATGTGAGAATTCGGAAAATGCCTGAGAACAGTGACTTGCTACCA GTTGCACAAACAGAGCCATCTATATGGACAGTTGATGATGTCTGGGCCTTCATCCATTCTTTGCCTGGCT GCCAGGATATCGCAGATGAATTCAGAGCACAGGAGATTGATGGACAGGCCCTTCTCTTGCTGAAAGAAGA CCATCTCATGAGTGCAATGAATATCAAGCTAGGCCCAGCCCTGAAGATCTGTGCACGCATCAACTCTCTG AAGGAATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217729 protein sequence
Red=Cloning site Green=Tags(s) MDTEPNPGTSSVSTTTSSTTTTTITTSSSRMQQPQISVYSGSDRHAVQVIQQALHRPPSSAAQYLQQMYA AQQQHLMLHTAALQQQHLSSSQLQSLAAVQASLSSGRPSTSPTGSVTQQSSMSQTSINLSTSPTPAQLIS RSQASSSTSGSITQQTMLLGSTSPTLTASQAQMYLRAQMLIFTPATTVAAVQSDIPVVSSSSSSSCQSAA TQVQNLTLRSQKLGVLSSSQNGPPKSTSQTQSLTICHNKTTVTSSKISQRDPSPESNKKGESPSLESRST AVTRTSSIHQLIAPASYSPIQPHSLIKHQQIPLHSPPSKVSHHQLILQQQQQQIQPITLQNSTQDPPPSQ HCIPLQNHGLPPAPSNAQSQHCSPIQSHPSPLTVSPNQSQSAQQSVVVSPPPPHSPSQSPTIIIHPQALI QPHPLVSSALQPGPNLQQSTANQVQATAQLNLPSHLPLPASPVVHIGPVQQSALVSPGQQIVSPSHQQYS SLQSSPIPIASPPQMSTSPPAQIPPLPLQSMQSLQVQPEILSQGQVLVQNALVSEEELPAAEALVQLPFQ TLPPPQTVAVNLQVQPPAPVDPPVVYQVEDVCEEEMPEESDECVRMDRTPPPPTLSPAAITVGRGEDLTS EHPLLEQVELPAVASVSASVIKSPSDPSHVSVPPPPLLLPAATTRSNSTSMHSSIPSIENKPPQAIVKPQ ILTHVIEGFVIQEGLEPFPVSRSSLLIEQPVKKRPLLDNQVINSVCVQPELQNNTKHADNSSDTEMEDMI AEETLEEMDSELLKCEFCGKMGYANEFLRSKRFCTMSCAKRYNVSCSKKFALSRWNRKPDNQSLGHRGRR PSGPDGAAREHILRQLPITYPSAEEDLASHEDSVPSAMTTRLRRQSERERERELRDVRIRKMPENSDLLP VAQTEPSIWTVDDVWAFIHSLPGCQDIADEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKICARINSL KES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024947 |
| ORF Size | 2949 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 12687 bp |
| RefSeq ORF | 2988 bp |
| Locus ID | 80012 |
| UniProt ID | Q8NDX5 |
| Domains | SAM |
| MW | 106.2 kDa |
| Gene Summary | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217729L1 | Lenti ORF clone of Human polyhomeotic homolog 3 (Drosophila) (PHC3), Myc-DDK-tagged |
CNY 13,200.00 |
|
| RC217729L2 | Lenti ORF clone of Human polyhomeotic homolog 3 (Drosophila) (PHC3), mGFP tagged |
CNY 9,030.00 |
|
| RC217729L3 | Lenti ORF clone of Human polyhomeotic homolog 3 (Drosophila) (PHC3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC217729L4 | Lenti ORF clone of Human polyhomeotic homolog 3 (Drosophila) (PHC3), mGFP tagged |
CNY 9,030.00 |
|
| RG217729 | PHC3 (tGFP-tagged) - Human polyhomeotic homolog 3 (Drosophila) (PHC3) |
CNY 12,400.00 |
|
| SC111403 | PHC3 (untagged)-Human polyhomeotic homolog 3 (Drosophila) (PHC3) |
CNY 40,352.00 |
|
| SC318436 | PHC3 (untagged)-Human polyhomeotic homolog 3 (Drosophila) (PHC3) |
CNY 7,220.00 |
