RPL7 (NM_000971) Human Tagged ORF Clone
CAT#: RC219242
RPL7 (Myc-DDK-tagged)-Human ribosomal protein L7 (RPL7)
ORF Plasmid: tGFP
"NM_000971" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | humL7-1; L7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219242 representing NM_000971
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTGTAGAAGAGAAGAAGAAGGAGGTTCCTGCTGTGCCAGAAACCCTTAAGAAAAAGCGAAGGA ATTTCGCAGAGCTGAAGATCAAGCGCCTGAGAAAGAAGTTTGCCCAAAAGATGCTTCGAAAGGCAAGGAG GAAGCTTATCTATGAAAAAGCAAAGCACTATCACAAGGAATATAGGCAGATGTACAGAACTGAAATTCGA ATGGCGAGGATGGCAAGAAAAGCTGGCAACTTCTATGTACCTGCAGAACCCAAATTGGCGTTTGTCATCA GAATCAGAGGTATCAATGGAGTGAGCCCAAAGGTTCGAAAGGTGTTGCAGCTTCTTCGCCTTCGTCAAAT CTTCAATGGAACCTTTGTGAAGCTCAACAAGGCTTCGATTAACATGCTGAGGATTGTAGAGCCATATATT GCATGGGGGTACCCCAATCTGAAGTCAGTAAATGAACTAATCTACAAGCGTGGTTATGGCAAAATCAATA AGAAGCGAATTGCTTTGACAGATAACGCTTTGATTGCTCGATCTCTTGGTAAATACGGCATCATCTGCAT GGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAAAGAGGCAAATAACTTCCTGTGGCCC TTCAAATTGTCTTCTCCACGAGGTGGAATGAAGAAAAAGACCACCCATTTTGTAGAAGGTGGAGATGCTG GCAACAGGGAGGACCAGATCAACAGGCTTATTAGAAGAATGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219242 representing NM_000971
Red=Cloning site Green=Tags(s) MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIR MARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYI AWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWP FKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000971 |
ORF Size | 744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000971.4 |
RefSeq Size | 866 bp |
RefSeq ORF | 747 bp |
Locus ID | 6129 |
UniProt ID | P18124 |
Domains | Ribosomal_L30 |
Protein Families | Transcription Factors |
Protein Pathways | Ribosome |
MW | 29 kDa |
Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219242L3 | Lenti ORF clone of Human ribosomal protein L7 (RPL7), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC219242L4 | Lenti ORF clone of Human ribosomal protein L7 (RPL7), mGFP tagged |
CNY 5,890.00 |
|
RG219242 | RPL7 (tGFP-tagged) - Human ribosomal protein L7 (RPL7) |
CNY 4,370.00 |
|
SC119573 | RPL7 (untagged)-Human ribosomal protein L7 (RPL7) |
CNY 2,400.00 |