LATS2 (NM_014572) Human Tagged ORF Clone
CAT＃: RC219394
LATS2 (Myc-DDK-tagged)-Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2)
ORF Plasmid: tGFP
"NM_014572" in other vectors (7)
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CNY 7576.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 1999.00
CNY 2700.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KPM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219394 representing NM_014572
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCAAAGACTTTTCCTGCCACGACTTATTCTGGAAATAGCCGGCAGCGACTGCAAGAGATTCGTG AGGGGTTAAAGCAGCCATCCAAGTCTTCGGTTCAGGGGCTACCCGCAGGACCAAACAGTGACACTTCCCT GGATGCCAAAGTCCTGGGGAGCAAAGATGCCACCAGGCAGCAGCAGCAGATGAGAGCCACCCCAAAGTTC GGACCTTATCAGAAAGCCTTGAGGGAAATCAGATATTCCTTGTTGCCTTTTGCTAATGAATCGGGCACCT CTGCAGCTGCAGAAGTGAACCGGCAAATGCTGCAGGAACTGGTGAACGCAGGATGCGACCAGGAGATGGC TGGCCGAGCTCTCAAGCAGACTGGCAGCAGGAGCATCGAGGCCGCCCTGGAGTACATCAGCAAGATGGGC TACCTGGACCCGAGGAATGAGCAGATTGTGCGGGTCATTAAGCAGACCTCCCCAGGAAAGGGGCTCATGC CAACCCCAGTGACGCGGAGGCCCAGCTTCGAAGGAACCGGCGATTCGTTTGCGTCCTACCACCAGCTGAG CGGTACCCCCTACGAGGGCCCAAGCTTCGGCGCTGACGGCCCCACGGCGCTGGAGGAGATGCCGCGGCCG TACGTGGACTACCTTTTCCCCGGAGTCGGCCCCCACGGGCCCGGCCACCAGCACCAGCACCCACCCAAGG GCTACGGTGCCAGCGTAGAGGCAGCAGGGGCACACTTCCCGCTGCAGGGCGCGCACTACGGGCGGCCGCA CCTGCTGGTGCCTGGGGAACCCCTGGGCTACGGAGTGCAGCGCAGCCCCTCCTTCCAGAGCAAGACGCCG CCGGAGACCGGGGGTTACGCCAGCCTGCCCACGAAGGGCCAGGGAGGACCGCCAGGCGCCGGCCTCGCTT TCCCACCCCCTGCCGCCGGGCTCTACGTGCCGCACCCACACCACAAGCAGGCCGGTCCCGCGGCCCACCA GCTGCATGTGCTGGGCTCCCGCAGCCAGGTGTTCGCCAGCGACAGCCCCCCGCAGAGCCTGCTCACTCCC TCGCGGAACAGCCTCAACGTGGACCTGTATGAATTGGGCAGCACCTCCGTCCAGCAGTGGCCGGCTGCCA CCCTGGCCCGCCGGGACTCCCTGCAGAAGCCGGGCCTGGAGGCGCCGCCGCGCGCGCACGTGGCCTTCCG GCCTGACTGCCCAGTGCCCAGCAGGACCAACTCCTTCAACAGCCACCAGCCGCGGCCCGGTCCGCCTGGC AAGGCCGAGCCCTCCCTGCCCGCCCCCAACACCGTGACGGCTGTCACGGCCGCGCACATCTTGCACCCGG TGAAGAGCGTGCGTGTGCTGAGGCCGGAGCCGCAGACGGCTGTGGGGCCCTCGCACCCCGCCTGGGTGCC CGCGCCTGCCCCGGCCCCCGCCCCCGCCCCCGCCCCGGCTGCGGAGGGCTTGGACGCCAAGGAGGAGCAT GCCCTGGCGCTGGGCGGCGCAGGCGCCTTCCCGCTGGACGTGGAGTACGGAGGCCCAGACCGGAGGTGCC CGCCTCCGCCCTACCCGAAGCACCTGCTGCTGCGCAGCAAGTCGGAGCAGTACGACCTGGACAGCCTGTG CGCAGGCATGGAGCAGAGCCTCCGTGCGGGCCCCAACGAGCCCGAGGGCGGCGACAAGAGCCGCAAAAGC GCCAAGGGGGACAAAGGCGGAAAGGATAAAAAGCAGATTCAGACCTCTCCCGTTCCCGTCCGCAAAAACA GCAGAGACGAAGAGAAGAGAGAGTCACGCATCAAGAGCTACTCGCCATACGCCTTTAAGTTCTTCATGGA GCAGCACGTGGAGAATGTCATCAAAACCTACCAGCAGAAGGTTAACCGGAGGCTGCAGCTGGAGCAAGAA ATGGCCAAAGCTGGACTCTGTGAAGCTGAGCAGGAGCAGATGCGGAAGATCCTCTACCAGAAAGAGTCTA ATTACAACAGGTTAAAGAGGGCCAAGATGGACAAGTCTATGTTTGTCAAGATCAAAACCCTGGGGATCGG TGCCTTTGGAGAAGTGTGCCTTGCTTGTAAGGTGGACACTCACGCCCTGTACGCCATGAAGACCCTAAGG AAAAAGGATGTCCTGAACCGGAATCAGGTGGCCCACGTCAAGGCCGAGAGGGACATCCTGGCCGAGGCAG ACAATGAGTGGGTGGTCAAACTCTACTACTCCTTCCAAGACAAAGACAGCCTGTACTTTGTGATGGACTA CATCCCTGGTGGGGACATGATGAGCCTGCTGATCCGGATGGAGGTCTTCCTTGAGCACCTGGCCCGGTTC TACATCGCAGAGCTGACTTTGGCCATTGAGAGTGTCCACAAGATGGGCTTCATCCACCGAGACATCAAGC CTGATAACATTTTGATAGATCTGGATGGTCACATTAAACTCACAGATTTCGGCCTCTGCACTGGGTTCAG GTGGACTCACAATTCCAAATATTACCAGAAAGGGAGCCATGTCAGACAGGACAGCATGGAGCCCAGCGAC CTCTGGGATGATGTGTCTAACTGTCGGTGTGGGGACAGGCTGAAGACCCTAGAGCAGAGGGCGCGGAAGC AGCACCAGAGGTGCCTGGCACATTCACTGGTGGGGACTCCAAACTACATCGCACCCGAGGTGCTCCTCCG CAAAGGGTACACTCAACTCTGTGACTGGTGGAGTGTTGGAGTGATTCTCTTCGAGATGCTGGTGGGGCAG CCGCCCTTTTTGGCACCTACTCCCACAGAAACCCAGCTGAAGGTGATCAACTGGGAGAACACGCTCCACA TTCCAGCCCAGGTGAAGCTGAGCCCTGAGGCCAGGGACCTCATCACCAAGCTGTGCTGCTCCGCAGACCA CCGCCTGGGGCGGAATGGGGCCGATGACCTGAAGGCCCACCCCTTCTTCAGCGCCATTGACTTCTCCAGT GACATCCGGAAGCAGCCAGCCCCCTACGTTCCCACCATCAGCCACCCCATGGACACCTCGAATTTCGACC CCGTAGATGAAGAAAGCCCTTGGAACGATGCCAGCGAAGGTAGCACCAAGGCCTGGGACACACTCACCTC GCCCAATAACAAGCATCCTGAGCACGCATTTTACGAATTCACCTTCCGAAGGTTCTTTGATGACAATGGC TACCCCTTTCGATGCCCAAAGCCTTCAGGAGCAGAAGCTTCACAGGCTGAGAGCTCAGATTTAGAAAGCT CTGATCTGGTGGATCAGACTGAAGGCTGCCAGCCTGTGTACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219394 protein sequence
Red=Cloning site Green=Tags(s) MRPKTFPATTYSGNSRQRLQEIREGLKQPSKSSVQGLPAGPNSDTSLDAKVLGSKDATRQQQQMRATPKF GPYQKALREIRYSLLPFANESGTSAAAEVNRQMLQELVNAGCDQEMAGRALKQTGSRSIEAALEYISKMG YLDPRNEQIVRVIKQTSPGKGLMPTPVTRRPSFEGTGDSFASYHQLSGTPYEGPSFGADGPTALEEMPRP YVDYLFPGVGPHGPGHQHQHPPKGYGASVEAAGAHFPLQGAHYGRPHLLVPGEPLGYGVQRSPSFQSKTP PETGGYASLPTKGQGGPPGAGLAFPPPAAGLYVPHPHHKQAGPAAHQLHVLGSRSQVFASDSPPQSLLTP SRNSLNVDLYELGSTSVQQWPAATLARRDSLQKPGLEAPPRAHVAFRPDCPVPSRTNSFNSHQPRPGPPG KAEPSLPAPNTVTAVTAAHILHPVKSVRVLRPEPQTAVGPSHPAWVPAPAPAPAPAPAPAAEGLDAKEEH ALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSEQYDLDSLCAGMEQSLRAGPNEPEGGDKSRKS AKGDKGGKDKKQIQTSPVPVRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQE MAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLACKVDTHALYAMKTLR KKDVLNRNQVAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFLEHLARF YIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSD LWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQ PPFLAPTPTETQLKVINWENTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFSS DIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPNNKHPEHAFYEFTFRRFFDDNG YPFRCPKPSGAEASQAESSDLESSDLVDQTEGCQPVYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_014572 |
| ORF Size | 3264 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014572.3 |
| RefSeq Size | 4098 bp |
| RefSeq ORF | 3267 bp |
| Locus ID | 26524 |
| UniProt ID | Q9NRM7 |
| Domains | UBA, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 120 kDa |
| Gene Summary | This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008] |
| Shipping | Ambient |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Expression of large tumour suppressor (LATS) kinases modulates chemotherapy response in advanced non-small cell lung cancer
,Luo, SY;Kwok, HH;Yang, PC;Ip, MS;Minna, JD;Lam, DC;,
Transl Lung Cancer Res
,PubMed ID 32420069
[LATS2]
|
|
Hypoxia-Induced Placenta-Specific microRNA (miR-512-3p) Promotes Hepatocellular Carcinoma Progression by Targeting Large Tumor Suppressor Kinase 2
,Zhang, B;Huang, L;Tu, J;Wu, T;,
OncoTargets and Therapy
,PubMed ID 32612368
[LATS2]
|
|
MiR-25 promotes ovarian cancer proliferation and motility by targeting LATS2
,Feng, S;Pan, W;Jin, Y;Zheng, J;,
Tumour Biol.
,PubMed ID 25179841
[LATS2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219394L1 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), Myc-DDK-tagged |
CNY 9976.00 |
|
| RC219394L2 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), mGFP tagged |
CNY 12730.00 |
|
| RC219394L3 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), Myc-DDK-tagged |
CNY 9976.00 |
|
| RC219394L4 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), mGFP tagged |
CNY 9976.00 |
|
| RG219394 | LATS2 (tGFP-tagged) - Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2) |
CNY 9176.00 |
|
| SC120535 | LATS2 (untagged)-Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2) |
CNY 11376.00 |
|
| SC323550 | LATS2 (untagged)-Kinase deficient mutant (K697M) of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2) |
CNY 11376.00 |
