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PLA2G4D (NM_178034) Human Tagged ORF Clone
CAT＃: RC222969
PLA2G4D (Myc-DDK-tagged)-Human phospholipase A2, group IVD (cytosolic) (PLA2G4D)
ORF Plasmid: tGFP
"NM_178034" in other vectors (5)
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CNY 5,992.00
CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | cPLA2delta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222969 representing NM_178034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCCTGTCACCTGGGGGACCACCTGGCCACCCTTACCAGGGGGAGGCCTCTACCTGCTGGCAGC TCACAGTGAGGGTCCTGGAGGCGCGGAACCTGCGCTGGGCTGACCTGTTGAGTGAGGCCGACCCTTACGT GATCCTACAGCTGTCGACCGCACCTGGAATGAAGTTTAAGACCAAGACGCTCACCGACACCAGTCATCCT GTGTGGAATGAGGCCTTCCGTTTCCTTATCCAAAGTCAGGTCAAGAATGTTCTGGAGCTTAGCATCTATG ATGAGGACTCAGTCACGGAGGATGACATCTGCTTCAAGGTTCTCTATGACATCTCAGAAGTCCTCCCTGG CAAGCTGCTCCGGAAAACCTTCTCCCAGAGTCCCCAGGGAGAGGAGGAGCTGGATGTGGAGTTCCTGATG GAAGAAACGTCAGATCGCCCAGAAAACCTCATCACCAACAAAGTCATTGTGGCCCGAGAGCTGTCATGCC TGGATGTGCATCTGGACAGCACAGGGAGCACCGCTGTGGTTGCAGATCAGGACAAGCTGGAGCTGGAGCT GGTGCTGAAGGGGTCCTATGAGGACACACAGACATCCTTCCTGGGCACAGCCTCTGCCTTCCGCTTCCAC TACATGGCAGCCCTAGAGACAGAGCTGAGCGGGCGCCTGAGGAGCTCCAGAAGCAATGGCTGGAATGGGG ACAACTCAGCTGGGTACCTCACTGTGCCCCTGAGGCCCTTGACCATTGGGAAGGAGGTGACTATGGATGT TCCTGCTCCAAATGCCCCAGGAGTGAGGCTGCAGCTCAAGGCAGAGGGCTGCCCTGAGGAGCTGGCCGTG CACCTGGGCTTCAATCTCTGTGCAGAGGAGCAGGCCTTCCTGAGCAGGAGGAAGCAGGTGGTGGCCAAGG CCCTGAAGCAGGCCCTGCAGCTGGACAGAGACCTGCAGGAGGATGAGGTACCCGTTGTGGGCATCATGGC CACAGGAGGAGGTGCCCGGGCCATGACCTCACTCTACGGCCACCTATTGGCCTTGCAGAAGCTGGGCCTC CTAGACTGTGTGACCTACTTCAGTGGCATCTCTGGCTCTACGTGGACAATGGCCCACCTGTACGGGGACC CTGAGTGGTCGCAGAGGGACCTGGAGGGACCTATCAGATACGCCCGGGAGCACCTGGCCAAGAGCAAGCT GGAGGTCTTTTCCCCAGAGCGCCTGGCGAGCTACCGCCGGGAGCTGGAGCTGCGGGCTGAGCAGGGCCAC CCCACGACCTTTGTGGACCTGTGGGCGCTAGTGCTGGAGTCCATGCTGCACGGCCAGGTGATGGATCAGA AGCTGTCAGGACAGAGAGCCGCCCTGGAACGGGGTCAGAACCCTCTGCCCCTCTACTTGAGCCTCAATGT CAAAGAGAACAATCTGGAGACACTGGACTTCAAGGAGTGGGTTGAGTTCTCCCCCTATGAGGTCGGTTTC CTGAAGTACGGGGCCTTCGTCCCTCCTGAGCTCTTCGGCTCCGAGTTCTTCATGGGACGGCTGATGAGGA GGATCCCGGAGCCCCGGATCTGCTTTCTGGAAGCCATCTGGAGCAACATTTTCTCCCTGAACCTGCTGGA TGCCTGGTATGACCTCACCAGTTCTGGGGAGTCCTGGAAACAGCACATCAAGGACAAGACCAGGAGCTTA GAGAAGGAGCCCCTGACCACCTCGGGGACCTCCTCGCGGCTGGAGGCCTCGTGGCTGCAGCCAGGCACGG CGCTGGCCCAGGCATTTAAAGGCTTCCTGACAGGCAGGCCCCTCCACCAGCGCAGCCCCAACTTCCTCCA GGGCCTCCAGCTGCACCAGGACTACTGTAGCCACAAAGACTTCTCCACCTGGGCAGACTACCAGCTTGAC TCCATGCCCAGCCAGCTGACCCCCAAGGAGCCCCGGCTCTGCCTGGTGGACGCCGCCTACTTCATCAACA CCAGCTCTCCCTCCATGTTCCGGCCAGGCCGCAGGCTGGACCTCATCCTCTCCTTCGACTACTCCCTATC TGCGCCCTTCGAGGCACTGCAGCAGACGGAGCTGTACTGCCGGGCCCGGGGGCTGCCCTTCCCCCGGGTG GAACCCAGCCCTCAGGACCAGCACCAGCCAAGGGAATGCCACCTCTTCTCAGACCCCGCCTGCCCCGAGG CCCCGATCCTGCTGCACTTCCCGCTGGTCAATGCCTCCTTCAAGGACCACTCAGCCCCCGGTGTCCAGCG CAGCCCCGCAGAGCTCCAGGGTGGCCAAGTGGATCTCACCGGGGCCACCTGCCCCTACACCCTGTCCAAC ATGACCTACAAGGAGGAAGACTTCGAGCGCCTGCTGCGGCTCAGTGACTACAACGTGCAGACCAGCCAGG GTGCCATCCTGCAGGCCCTGAGGACCGCGCTGAAGCACCGGACTCTAGAGGCGAGGCCTCCAAGGGCACA GACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222969 representing NM_178034
Red=Cloning site Green=Tags(s) MESLSPGGPPGHPYQGEASTCWQLTVRVLEARNLRWADLLSEADPYVILQLSTAPGMKFKTKTLTDTSHP VWNEAFRFLIQSQVKNVLELSIYDEDSVTEDDICFKVLYDISEVLPGKLLRKTFSQSPQGEEELDVEFLM EETSDRPENLITNKVIVARELSCLDVHLDSTGSTAVVADQDKLELELVLKGSYEDTQTSFLGTASAFRFH YMAALETELSGRLRSSRSNGWNGDNSAGYLTVPLRPLTIGKEVTMDVPAPNAPGVRLQLKAEGCPEELAV HLGFNLCAEEQAFLSRRKQVVAKALKQALQLDRDLQEDEVPVVGIMATGGGARAMTSLYGHLLALQKLGL LDCVTYFSGISGSTWTMAHLYGDPEWSQRDLEGPIRYAREHLAKSKLEVFSPERLASYRRELELRAEQGH PTTFVDLWALVLESMLHGQVMDQKLSGQRAALERGQNPLPLYLSLNVKENNLETLDFKEWVEFSPYEVGF LKYGAFVPPELFGSEFFMGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSL EKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLD SMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEALQQTELYCRARGLPFPRV EPSPQDQHQPRECHLFSDPACPEAPILLHFPLVNASFKDHSAPGVQRSPAELQGGQVDLTGATCPYTLSN MTYKEEDFERLLRLSDYNVQTSQGAILQALRTALKHRTLEARPPRAQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178034 |
| ORF Size | 2454 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178034.4 |
| RefSeq Size | 3584 bp |
| RefSeq ORF | 2457 bp |
| Locus ID | 283748 |
| UniProt ID | Q86XP0 |
| Protein Pathways | Fc gamma R-mediated phagocytosis |
| MW | 91.8 kDa |
| Gene Summary | The phospholipase A2 enzyme family, including PLA2G4D, catalyze the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids (Chiba et al., 2004 [PubMed 14709560]).[supplied by OMIM, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222969L3 | Lenti ORF clone of Human phospholipase A2, group IVD (cytosolic) (PLA2G4D), Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC222969L4 | Lenti ORF clone of Human phospholipase A2, group IVD (cytosolic) (PLA2G4D), mGFP tagged |
CNY 10,550.00 |
|
| RG222969 | PLA2G4D (tGFP-tagged) - Human phospholipase A2, group IVD (cytosolic) (PLA2G4D) |
CNY 7,592.00 |
|
| SC123354 | PLA2G4D (untagged)-Human phospholipase A2, group IVD (cytosolic) (PLA2G4D) |
CNY 2,400.00 |
|
| SC318298 | PLA2G4D (untagged)-Human phospholipase A2, group IVD (cytosolic) (PLA2G4D) |
CNY 15,680.00 |
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