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WIPI2 (NM_001033519) Human Tagged ORF Clone
CAT＃: RC224661
WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 4
ORF Plasmid: tGFP
"NM_001033519" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224661 representing NM_001033519
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTGGCGAGCCAGAGCGGGGAGGCCGGCGCCGGCCAGCTGCTCTTCGCCAACTTCAACCAGGACA ACACGTCCCTAGCTGTTGGTAGTAAGTCCGGTTATAAATTTTTCTCCCTTTCTTCTGTGGATAAGCTGGA ACAGATCTATGAATGCACCGATACGGAAGATGTGTGCATTGTAGAGAGATTGTTCTCCAGCAGCCTAGTG GCCATCGTCAGCCTTAAAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAAGGGAACTGAGATCTGCA ACTACAGCTACTCCAACACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTGATAGTATGCCTGGAGGA GTCCCTGTACATCCACAACATTCGGGACATGAAGGTGCTGCATACGATCAGGGAGACGCCTCCAAACCCT GCAGGCCTGTGTGCGCTGTCAATCAACAACGACAACTGCTACTTGGCGTACCCAGGGAGCGCGACCATCG GAGAGGTGCAGGTCTTCGATACCATTAATTTGAGAGCTGCAAACATGATTCCGGCTCACGACAGTCCTTT AGCGGCACTGGCCTTTGACGCAAGTGGAACTAAACTTGCCACGGCTTCGGAGAAGGGGACCGTGATTAGG GTATTTTCCATTCCAGAAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAAGAGGTGCGTGAGCATCT GCTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACTGAGACCGTGCACATCTT CAAACTCGAGACTGTGAAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCGGGTACTTCGGGAAAGTG CTCATGGCCTCCACCAGCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCAGGGCAGAGCCTTCGCCA CGGTCCGCCTGCCATTCTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATTCAGAAGATCCCGCGGTT GTTGGTGGGTGCCGCCGACGGGTACCTGTACATGTACAACCTGGACCCCCAGGAGGGCGGCGAGTGTGCC CTGATGAAGCAGCACCGGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTTGGACTCTGCCTCTCACG ACTGCCCCTTAGTCACTCAGACATACGGCGCAGCTGCAGGAAAAGCCTACACAGACGACCTGGGTGCTGT GGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACAGCGAGCACCCGCCCATG ATTCTTCGGACTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224661 representing NM_001033519
Red=Cloning site Green=Tags(s) MNLASQSGEAGAGQLLFANFNQDNTSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLV AIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNP AGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIR VFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETVKEKPPEEPTTWTGYFGKV LMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAADGYLYMYNLDPQEGGECA LMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKAYTDDLGAVGGACLEDEASALRLDEDSEHPPM ILRTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033519 |
| ORF Size | 1275 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033519.2 |
| RefSeq Size | 4402 bp |
| RefSeq ORF | 1278 bp |
| Locus ID | 26100 |
| UniProt ID | Q9Y4P8 |
| MW | 46.2 kDa |
| Gene Summary | WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224661L3 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224661L4 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RG224661 | WIPI2 (tGFP-tagged) - Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 4 |
CNY 4,370.00 |
|
| SC302716 | WIPI2 (untagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 4 |
CNY 6,840.00 |
