MYO18A (NM_078471) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAJN; MYSPDZ; SP-R210; SPR210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC224962 representing NM_078471 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTAACCTAATGAAGAAAGACAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAGAAAAAGGAGA AAAAGGAGCGGATGTCAGCGGCAGAGCTTCGGAGCCTGGAGGAGATGAGCCTGCGACGTGGCTTCTTCAA CCTGAACCGCTCCTCCAAGCGTGAATCCAAGACGCGCCTGGAAATCTCCAACCCCATCCCCATCAAGGTG GCCAGCGGCTCTGACCTGCACCTGACTGACATTGACTCCGATAGTAACCGGGGCAGCGTCATCCTGGACT CGGGCCACCTAAGTACAGCCAGCTCCAGCGATGACCTCAAGGGTGAGGAGGGTAGCTTCCGTGGCTCGGT GCTGCAGCGGGCAGCCAAGTTCGGCTCACTGGCCAAGCAGAACTCACAGATGATTGTCAAGCGCTTTTCC TTCTCCCAGCGTAGCCGGGATGAGAGCGCCTCAGAAACCTCGACGCCCTCAGAGCACTCTGCCGCCCCCT CGCCACAGGTGGAGGTGAGGACTCTAGAGGGACAGCTGGTGCAGCATCCTGGCCCAGGCATCCCTCGACC AGGGCACCGATCCCGAGCCCCTGAGCTAGTGACTAAAAAGTTCCCAGTCGACCTGCGCCTGCCCCCCGTG GTGCCCCTGCCCCCACCTACCCTCCGGGAGCTGGAGCTGCAACGACGGCCCACTGGAGACTTTGGCTTCT CCCTGCGGCGCACAACCATGCTGGATCGGGGCCCCGAGGGCCAGGCCTGTCGGCGTGTGGTCCACTTTGC TGAGCCTGGTGCAGGCACCAAGGACCTGGCCCTGGGGCTGGTGCCAGGAGATCGACTGGTGGAGATTAAT GGGCACAATGTGGAGAGCAAGTCCAGGGATGAGATTGTGGAGATGATCCGGCAGTCAGGGGACAGCGTGC GGCTCAAGGTGCAGCCCATTCCAGAGCTCAGCGAGCTCAGCAGGAGCTGGCTGCGGAGCGGCGAGGGACC TCGCAGGGAGCCATCCGATGCGAAAACAGAAGAACAGATTGCAGCAGAAGAGGCCTGGAATGAGACGGAG AAGGTGTGGCTGGTCCATAGGGACGGCTTCTCACTGGCCAGTCAACTCAAATCTGAGGAGCTCAACTTGC CTGAGGGGAAGGTGCGTGTGAAGCTGGACCACGATGGGGCCATCCTGGATGTGGATGAGGATGACGTTGA GAAGGCTAATGCTCCCTCCTGCGACCGTCTGGAGGATCTGGCCTCACTGGTGTACCTCAATGAGTCCAGC GTCCTGCACACCTTGCGCCAGCGCTATGGCGCTAGCCTGCTGCACACGTATGCTGGCCCCAGCCTGCTGG TTCTTGGCCCCCGTGGGGCCCCTGCTGTGTACTCTGAGAAGGTGATGCACATGTTCAAGGGTTGTCGGCG GGAGGACATGGCACCCCACATCTATGCAGTGGCCCAGACCGCATACAGGGCGATGCTGATGAGCCGTCAG GATCAGTCAATCATCCTCCTGGGCAGTAGTGGCAGTGGCAAGACCACCAGCTGCCAGCATCTGGTGCAGT ACCTGGCCACCATCGCGGGCATCAGCGGGAACAAGGTGTTTTCTGTGGAGAAGTGGCAGGCTCTGTACAC CCTCCTGGAAGCCTTTGGGAACAGCCCCACCATCATTAATGGCAATGCCACCCGCTTCTCCCAGATCCTC TCCCTGGACTTTGACCAAGCTGGCCAGGTGGCCTCAGCCTCCATTCAGACAATGCTTCTGGAGAAGCTGC GTGTGGCTCGGCGCCCAGCCAGTGAAGCCACATTCAACGTCTTCTACTACCTGCTGGCCTGTGGGGATGG CACCCTCAGGACAGAGCTCCACCTCAACCACTTGGCAGAGAACAATGTGTTTGGGATTGTGCCACTGGCC AAGCCTGAGGAAAAGCAGAAGGCAGCTCAGCAGTTTAGTAAGCTGCAGGCGGCCATGAAGGTGCTGGGCA TCTCCCCCGATGAACAGAAGGCCTGCTGGTTCATTCTGGCTGCCATCTACCACCTGGGGGCTGCGGGAGC CACCAAAGAAGCTGCTGAAGCTGGGCGCAAGCAGTTTGCCCGCCATGAGTGGGCCCAGAAGGCTGCGTAC CTACTGGGCTGCAGCCTGGAGGAGCTGTCCTCAGCCATCTTCAAGCACCAGCACAAGGGTGGCACCCTGC AGCGCTCCACCTCCTTCCGCCAGGGCCCCGAGGAGAGTGGCCTGGGAGATGGGACAGGCCCGAAACTGAG TGCACTGGAGTGCCTTGAGGGCATGGCGGCCGGCCTCTACAGCGAGCTCTTCACCCTTCTCGTCTCCCTG GTGAATAGGGCTCTCAAGTCCAGCCAGCACTCACTCTGCTCCATGATGATTGTCGACACCCCGGGCTTCC AGAACCCTGAGCAGGGTGGGTCAGCCCGCGGAGCCTCCTTTGAGGAGCTGTGCCACAACTACACCCAAGA CCGGCTGCAGAGGCTCTTCCACGAGCGCACCTTCGTGCAGGAGTTGGAAAGATACAAGGAGGAGAACATC GAGCTGGCGTTTGACGACTTGGAACCCCCGACGGATGACTCTGTGGCTGCTGTGGACCAGGCCTCCCATC AGTCCCTGGTCCGCTCGCTGGCCCGCACAGACGAGGCGAGGGGCCTGCTCTGGCTATTGGAAGAGGAGGC TCTGGTGCCAGGGGCCAGTGAGGACACCCTCCTGGAGCGCCTTTTCTCCTATTATGGCCCCCAGGAAGGT GACAAAAAAGGCCAAAGCCCCCTTCTGCACAGCAGCAAACCACACCACTTTCTCCTGGGCCACAGCCATG GCACCAACTGGGTAGAGTACAATGTGACTGGCTGGCTGAACTACACCAAGCAGAACCCAGCCACCCAGAA TGCCCCCCGGCTCCTGCAGGACTCCCAGAAAAAAATCATCAGCAACCTGTTTCTGGGCCGCGCAGGCAGT GCCACGGTGCTCTCTGGCTCCATCGCGGGCCTGGAGGGCGGCTCGCAGCTGGCACTGCGCCGGGCCACCA GCATGCGGAAAACCTTTACCACAGGCATGGCGGCTGTCAAAAAGAAGTCACTGTGCATCCAGATGAAGCT ACAGGTGGACGCCCTCATCGACACCATCAAGAAGTCAAAGCTGCATTTTGTGCACTGCTTCCTGCCTGTA GCTGAGGGCTGGGCTGGGGAGCCCCGTTCCGCCTCCTCCCGCCGAGTCAGCAGCAGCAGTGAGCTGGACC TGCCCTCGGGAGACCACTGCGAGGCTGGGCTCCTGCAGCTCGACGTGCCCCTGCTCCGCACCCAGCTCCG CGGCTCCCGCCTGCTCGATGCCATGCGCATGTACCGCCAAGGTTACCCTGACCACATGGTGTTTTCCGAG TTCCGCCGCCGCTTTGATGTCCTGGCCCCGCACCTGACCAAGAAACACGGGCGTAACTACATCGTGGTGG ATGAAAGGCGGGCAGTGGAGGAGCTGCTGGAGTGCTTGGATCTGGAGAAGAGCAGCTGCTGCATGGGCCT GAGCCGGGTGTTCTTCCGGGCGGGCACCTTGGCACGGCTGGAGGAGCAGCGGGATGAACAAACCAGCAGG AACCTAACCCTGTTCCAAGCAGCCTGCAGGGGCTACCTGGCCCGCCAGCACTTCAAGAAGAGAAAGATCC AGGACCTGGCCATTCGCTGTGTACAGAAGAACATCAAGAAGAACAAAGGGGTGAAGGACTGGCCCTGGTG GAAGCTTTTTACCACAGTGAGGCCCCTCATCGAAGTACAGCTGTCAGAGGAGCAGATCCGGAACAAAGAC GAGGAGATCCAGCAGCTGCGGAGCAAGCTCGAGAAGGCGGAGAAGGAGAGGAACGAGCTGCGGCTCAACA GTGACCGGCTGGAGAGCCGGATCTCAGAGCTGACATCGGAGCTGACAGATGAGCGTAACACAGGAGAGTC CGCCTCCCAGCTGCTGGACGCGGAGACAGCAGAGAGGCTCCGGGCTGAGAAGGAGATGAAGGAACTGCAG ACCCAGTACGATGCACTGAAGAAGCAGATGGAGGTTATGGAAATGGAGGTGATGGAGGCCCGTCTCATCC GGGCAGCGGAGATCAACGGGGAAGTGGATGATGATGATGCAGGTGGCGAGTGGCGGCTGAAGTATGAGCG GGCTGTGCGGGAGGTGGACTTCACCAAGAAACGGCTCCAGCAGGAGTTTGAGGACAAGCTGGAGGTGGAG CAGCAGAACAAGAGGCAGCTGGAACGGCGGCTCGGGGACCTGCAGGCAGATAGTGAGGAGAGTCAGCGGG CTCTGCAGCAGCTCAAGAAGAAGTGCCAGCGACTGACGGCTGAGCTGCAAGACACCAAGCTGCACCTGGA GGGCCAGCAGGTCCGCAACCACGAACTGGAGAAGAAGCAGAGGAGGTTTGACAGTGAGCTCTCGCAGGCG CATGAGGAGGCCCAGCGGGAGAAGCTGCAGCGGGAGAAGCTGCAGCGGGAGAAGGACATGCTCCTCGCTG AGGCTTTCAGCCTGAAGCAGCAACTAGAGGAAAAAGACATGGACATTGCAGGGTTCACCCAGAAGGTTGT GTCTCTAGAGGCAGAGCTCCAGGACATTTCTTCCCAAGAGTCCAAGGATGAGGCTTCTCTGGCCAAGGTC AAGAAACAGCTCCGGGACCTGGAGGCCAAAGTCAAGGATCAGGAAGAAGAGCTGGATGAGCAGGCAGGGA CCATCCAGATGCTGGAACAGGCCAAGCTGCGTCTGGAGATGGAGATGGAGCGGATGAGACAGACCCATTC TAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAGGAGGCCCGGCAGTCGTGTCAGAAGAAGTTAAAACAG ATGGAGGTGCAGCTAGAGGAAGAGTATGAGGACAAGCAGAAGGTTCTGCGAGAGAAGCGGGAGCTGGAGG GCAAGCTCGCCACCCTCAGCGACCAGGTGAACCGGCGGGACTTTGAGTCAGAGAAGCGGCTGCGGAAGGA CCTGAAGCGCACCAAGGCCCTGCTGGCAGATGCCCAGCTCATGCTGGACCACCTGAAGAACAGTGCTCCC AGCAAGCGAGAGATTGCCCAGCTCAAGAACCAGCTGGAGGAGTCAGAGTTCACCTGTGCGGCAGCCGTGA AAGCACGGAAAGCAATGGAGGTGGAGATCGAAGACCTGCACCTGCAGATTGATGACATCGCCAAAGCCAA GACAGCGCTGGAGGAGCAGCTGAGCCGCCTTCAGCGTGAGAAGAATGAGATCCAGAACCGGCTGGAGGAA GATCAGGAAGACATGAACGAATTGATGAAGAAGCACAAGGCTGCCGTGGCTCAGGCTTCCCGGGACCTGG CTCAGATAAATGATCTCCAAGCTCAGCTAGAAGAAGCCAACAAAGAGAAGCAGGAGCTGCAGGAGAAGCT ACAAGCCCTCCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTGGACAAGTCCCTGGTGAGCAGGCAG GAAGCTAAGATACGGGAGCTGGAGACACGCCTGGAGTTTGAAAGGACGCAAGTGAAACGGCTGGAGAGCC TGGCTAGCCGTCTCAAGGAAAACATGGAGAAGCTGACTGAGGAGCGGGATCAGCGCATTGCAGCCGAGAA CCGGGAGAAGGAACAGAACAAGCGGCTACAGAGGCAGCTCCGGGACACCAAGGAGGAGATGGGCGAGCTT GCCAGGAAGGAGGCCGAGGCGAGCCGCAAGAAGCACGAACTGGAGATGGATCTAGAAAGCCTGGAGGCTG CTAACCAGAGCCTGCAGGCTGACCTAAAGTTGGCATTCAAGCGCATCGGGGACCTGCAGGCTGCCATTGA GGATGAGATGGAGAGTGATGAGAATGAGGACCTCATCAACAGTTTGCAGGACATGGTGACAAAGTATCAG AAAAGAAAGAATAAACTTGAGGGAGACTCTGATGTGGACTCGGAGCTGGAGGACCGTGTTGACGGGGTCA AGTCCTGGTTGTCAAAAAACAAGGGACCTTCCAAGGCAGCTTCTGATGATGGCAGCTTAAAGAGTTCCAG CCCCACCAGCTACTGGAAGTCCCTTGCCCCTGATCGGTCAGATGATGAGCACGACCCTCTCGACAACACC TCCAGACCGCGATACTCCCACAGTTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACGGAGACTAACG CA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC224962 representing NM_078471 Red=Cloning site Green=Tags(s) MFNLMKKDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMSLRRGFFNLNRSSKRESKTRLEISNPIPIKV ASGSDLHLTDIDSDSNRGSVILDSGHLSTASSSDDLKGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFS FSQRSRDESASETSTPSEHSAAPSPQVEVRTLEGQLVQHPGPGIPRPGHRSRAPELVTKKFPVDLRLPPV VPLPPPTLRELELQRRPTGDFGFSLRRTTMLDRGPEGQACRRVVHFAEPGAGTKDLALGLVPGDRLVEIN GHNVESKSRDEIVEMIRQSGDSVRLKVQPIPELSELSRSWLRSGEGPRREPSDAKTEEQIAAEEAWNETE KVWLVHRDGFSLASQLKSEELNLPEGKVRVKLDHDGAILDVDEDDVEKANAPSCDRLEDLASLVYLNESS VLHTLRQRYGASLLHTYAGPSLLVLGPRGAPAVYSEKVMHMFKGCRREDMAPHIYAVAQTAYRAMLMSRQ DQSIILLGSSGSGKTTSCQHLVQYLATIAGISGNKVFSVEKWQALYTLLEAFGNSPTIINGNATRFSQIL SLDFDQAGQVASASIQTMLLEKLRVARRPASEATFNVFYYLLACGDGTLRTELHLNHLAENNVFGIVPLA KPEEKQKAAQQFSKLQAAMKVLGISPDEQKACWFILAAIYHLGAAGATKEAAEAGRKQFARHEWAQKAAY LLGCSLEELSSAIFKHQHKGGTLQRSTSFRQGPEESGLGDGTGPKLSALECLEGMAAGLYSELFTLLVSL VNRALKSSQHSLCSMMIVDTPGFQNPEQGGSARGASFEELCHNYTQDRLQRLFHERTFVQELERYKEENI ELAFDDLEPPTDDSVAAVDQASHQSLVRSLARTDEARGLLWLLEEEALVPGASEDTLLERLFSYYGPQEG DKKGQSPLLHSSKPHHFLLGHSHGTNWVEYNVTGWLNYTKQNPATQNAPRLLQDSQKKIISNLFLGRAGS ATVLSGSIAGLEGGSQLALRRATSMRKTFTTGMAAVKKKSLCIQMKLQVDALIDTIKKSKLHFVHCFLPV AEGWAGEPRSASSRRVSSSSELDLPSGDHCEAGLLQLDVPLLRTQLRGSRLLDAMRMYRQGYPDHMVFSE FRRRFDVLAPHLTKKHGRNYIVVDERRAVEELLECLDLEKSSCCMGLSRVFFRAGTLARLEEQRDEQTSR NLTLFQAACRGYLARQHFKKRKIQDLAIRCVQKNIKKNKGVKDWPWWKLFTTVRPLIEVQLSEEQIRNKD EEIQQLRSKLEKAEKERNELRLNSDRLESRISELTSELTDERNTGESASQLLDAETAERLRAEKEMKELQ TQYDALKKQMEVMEMEVMEARLIRAAEINGEVDDDDAGGEWRLKYERAVREVDFTKKRLQQEFEDKLEVE QQNKRQLERRLGDLQADSEESQRALQQLKKKCQRLTAELQDTKLHLEGQQVRNHELEKKQRRFDSELSQA HEEAQREKLQREKLQREKDMLLAEAFSLKQQLEEKDMDIAGFTQKVVSLEAELQDISSQESKDEASLAKV KKQLRDLEAKVKDQEEELDEQAGTIQMLEQAKLRLEMEMERMRQTHSKEMESRDEEVEEARQSCQKKLKQ MEVQLEEEYEDKQKVLREKRELEGKLATLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAP SKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEE DQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQ EAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGEL ARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSLQDMVTKYQ KRKNKLEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNT SRPRYSHSYLSDSDTEAKLTETNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_078471
ORF Size	6162 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_078471.4
RefSeq Size	7591 bp
RefSeq ORF	6165 bp
Locus ID	399687
UniProt ID	Q92614
MW	232.9 kDa
Gene Summary	The protein encoded by this gene can bind GOLPH3, linking the Golgi to the cytoskeleton and influencing Golgi membrane trafficking. The encoded protein is also part of a complex that assembles lamellar actomyosin bundles and may be required for cell migration. [provided by RefSeq, Oct 2016]

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