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USP20 (NM_001110303) Human Tagged ORF Clone
CAT＃: RC226260
- TrueORF®
USP20 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 3
ORF Plasmid: tGFP
"NM_001110303" in other vectors (5)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hVDU2; LSFR3A; VDU2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226260 representing NM_001110303
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGGAGAGGTGACCAAAGAGGACTTGCTGC TCAAATCTAAGGGAACCTGTCAGTCGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGC CTGCCCCTATGTTGGCTGCGGAGAATCCTTTGCTGACCACAGCACCATTCATGCACAGGCAAAAAAGCAC AACTTGACCGTGAACCTGACCACGTTCCGACTGTGGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGC AGCGGCTGGCAGCCCCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACCCTCCCA CCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGTCTGAGTCAGAGGACGATGACCTGAAA CCTCGAGGCCTCACGGGCATGAAGAACCTCGGGAACTCCTGCTACATGAACGCTGCCCTGCAGGCCCTGT CCAATTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCACAGATAAGAAGCCAGC CCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAGGTCTGGCATAAGAAACGGCCAAGCTACGTGGTCCCC ACCAGTCTGTCTCATGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAGGACACCCAAG AGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAGGAGCCGGTGGTGGCCACGGTGGCGCT GACGGAGGCTCGGGACTCAGATTCGAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAA GATGAGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAGGGGCGTGGCGGGGGCA GCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGATGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGAT GAAGGACCGCAAGTTCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCTGATGTG GACACTGCCATGGCTGCCCTTGACGACCAGCCCGCGGAGGCCCAGCCCCCGTCACCACGGTCCTCCAGCC CCTGCCGGACGCCAGAGCCGGACAATGATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCA CCACCACGAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAGGATGGCACCGTCG TACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCAGCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCG TCATCTCAGACATCTTTGACGGCTCCATTCTCAGCCTTGTGCAGTGTCTCACCTGTGACCGGGTATCCAC CACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGGAGGACCTGGCCAAGCTCCATTCA GCCATCTACCAGAATGTGCCGGCCAAGCCAGGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGG CCTTCATTGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTTGGGGGCCTGTCGT CACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCTGATGAGTTAAAGGGTGACAACATGTACAGCTGT GAGCGGTGTAAGAAGCTGCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGTGCA TTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATCAACAGCCACGTCTCCTTCCCCCT CGAGGGGCTCGACCTGCGCCCCTTCCTTGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTC TCGGTCATCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAGAACGTGATCAATG GGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGAAGTCCACGAGACGGTGGTGCAGAACGCCGAGGG CTACGTACTCTTCTACAGGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTGGCC GCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTGGCTCAACAAGTTCAACACCTTCG CGGAGCCAGGCCCCATCACCAACCAGACCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCA CTACATCGACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAACAGATTCGGGGGT GGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCTGCCAGGTGGAGATCGAGGCACTGGCCAAGCGCA GGAGGATCGAGATCGACACCTTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGTCAT CTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCAAGGGGAAGGACAACGAGCCCCCC GGGCCCATTGACAACAGCAGGATTGCACAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTG ACTACGGGCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTGGCCCCGAGATTGC CATCCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAGAACCTGCACGGGGAGCAGAAGATCGAAGCCGAG ACGCGGGCCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226260 representing NM_001110303
Red=Cloning site Green=Tags(s) MGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKKH NLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVADEGESESEDDDLK PRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVP TSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRSPSE DEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADV DTAMAALDDQPAEAQPPSPRSSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPRASPVRMAPS YVLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHS AIYQNVPAKPGACGDSYAAQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSC ERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRPFLAKECTSQITTYDLL SVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEGYVLFYRKSSEEAMRERQQVVSLA AMREPSLLRFYVSREWLNKFNTFAEPGPITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVWEHLYNRFGG GPAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESPGVIYCISMQWFREWEAFVKGKDNEPP GPIDNSRIAQVKGSGHVQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQPLGPENLHGEQKIEAE TRAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001110303 |
| ORF Size | 2742 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001110303.4 |
| RefSeq Size | 4361 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 10868 |
| UniProt ID | Q9Y2K6 |
| Protein Families | Druggable Genome, Protease |
| MW | 102 kDa |
| Gene Summary | This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226260L3 | Lenti-ORF clone of USP20 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 3 |
CNY 5,890.00 |
|
| RC226260L4 | Lenti-ORF clone of USP20 (mGFP-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 3 |
CNY 5,890.00 |
|
| RG226260 | USP20 (tGFP-tagged) - Human ubiquitin specific peptidase 20 (USP20), transcript variant 3 |
CNY 4,370.00 |
|
| SC317209 | USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 3 |
CNY 7,220.00 |
|
| SC328028 | USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20) transcript variant 3 |
CNY 7,220.00 |
