KLHL2 (NM_001161522) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ABP-KELCH; MAV; MAYVEN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC228934 representing NM_001161522 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGAGCCGAGCAAAGAGAGTTAGAATAAAAGAGGTAGATGGCTGGACCCTGAGGATGCTAATTG ATTATGTTTACACTGCAGAAATTCAGGTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCT CTTACAGTTACAGGATGTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTCAACTGC TTAGGAATCCGGGCTTTTGCTGATATGCATGCATGTACCGACCTTCTGAACAAGGCCAACACCTATGCAG AGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAATCTTGGCATCGAACAAGTGTGCAGCTT AATCTCAAGTGACAAACTTACCATTTCTTCAGAAGAGAAGGTATTTGAAGCAGTAATAGCATGGGTGAAC CATGACAAGGATGTGAGGCAAGAGTTTATGGCCCGACTGATGGAACATGTACGGTTACCTTTGCTTCCTC GGGAATATTTAGTTCAGAGGGTTGAAGAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCT CATTGAAGCAATGAAGTACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGGACCCGG CTGAGGACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCACCAAAGGCTATCCGGA GTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTAGCAGAGTTGCCTTCCAGGAGGTGCAG GGCAGGCATGGTCTACATGGCTGGACTTGTTTTTGCTGTTGGTGGCTTTAATGGCTCATTAAGAGTTCGC ACTGTAGATTCCTACGACCCTGTGAAGGACCAGTGGACCAGCGTTGCTAACATGAGAGACCGGAGAAGCA CTTTGGGAGCTGCTGTGTTAAATGGATTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTC ATCTGTGGAAGCATACAACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGGAGGAGC AGTGTTGGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGATGGAGCATCACGTCAGT GTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGGACCTATATAGCAGAAATGAGCACCAG GCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAATTTATTGTATGCTGTAGGAGGTCATGATGGCCCTTTA GTACGAAAAAGTGTTGAAGTATATGATCCCACCACTAACGCATGGAGACAGGTTGCAGATATGAACATGT GCAGAAGAAATGCAGGAGTTTGTGCAGTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTG TAACTTGGCGTCAGTAGAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGTATGAGC ACAGGGAGAAGTTATGCAGGGGTCACAGTTATTGATAAACCATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC228934 representing NM_001161522 Red=Cloning site Green=Tags(s) MSESRAKRVRIKEVDGWTLRMLIDYVYTAEIQVTEENVQVLLPAAGLLQLQDVKKTCCEFLESQLHPVNC LGIRAFADMHACTDLLNKANTYAEQHFADVVLSEEFLNLGIEQVCSLISSDKLTISSEEKVFEAVIAWVN HDKDVRQEFMARLMEHVRLPLLPREYLVQRVEEEALVKNSSACKDYLIEAMKYHLLPTEQRILMKSVRTR LRTPMNLPKLMVVVGGQAPKAIRSVECYDFKEERWHQVAELPSRRCRAGMVYMAGLVFAVGGFNGSLRVR TVDSYDPVKDQWTSVANMRDRRSTLGAAVLNGLLYAVGGFDGSTGLSSVEAYNIKSNEWFHVAPMNTRRS SVGVGVVGGLLYAVGGYDGASRQCLSTVECYNATTNEWTYIAEMSTRRSGAGVGVLNNLLYAVGGHDGPL VRKSVEVYDPTTNAWRQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPTTDKWTVVSSCMS TGRSYAGVTVIDKPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001161522
ORF Size	1515 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001161522.1, NP_001154994.1
RefSeq Size	2907 bp
RefSeq ORF	1518 bp
Locus ID	11275
UniProt ID	O95198
MW	56.1 kDa
Gene Summary	Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation (By similarity). Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4. Plays a role in the reorganization of the actin cytoskeleton. Promotes growth of cell projections in oligodendrocyte precursors.[UniProtKB/Swiss-Prot Function]

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