KCNQ3 (NM_001204824) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BFNC2; EBN2; KV7.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234832 representing NM_001204824 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCTGCAGAACACGCCACGATGTTCCTGATTGTCCTGGGGTGCTTGATTCTGGCTGTCCTGACCA CATTCAAGGAGTATGAGACTGTCTCGGGAGACTGGCTTCTGTTACTGGAGACATTTGCTATTTTCATCTT TGGAGCCGAGTTTGCTTTGAGGATCTGGGCTGCTGGATGTTGCTGCCGATACAAAGGCTGGCGGGGCCGA CTGAAGTTTGCCAGGAAGCCCCTGTGCATGTTGGACATCTTTGTGCTGATTGCCTCTGTGCCAGTGGTTG CTGTGGGAAACCAAGGCAATGTTCTGGCCACCTCCCTGCGAAGCCTGCGCTTCCTGCAGATCCTGCGCAT GCTGCGGATGGACCGGAGAGGTGGCACCTGGAAGCTTCTGGGCTCAGCCATCTGTGCCCACAGCAAAGAA CTCATCACGGCCTGGTACATCGGTTTCCTGACACTCATCCTTTCTTCATTTCTTGTCTACCTGGTTGAGA AAGACGTCCCAGAGGTGGATGCACAAGGAGAGGAGATGAAAGAGGAGTTTGAGACCTATGCAGATGCCCT GTGGTGGGGCCTGATCACACTGGCCACCATTGGCTATGGAGACAAGACACCCAAAACGTGGGAAGGCCGT CTGATTGCCGCCACCTTTTCCTTAATTGGCGTCTCCTTTTTTGCCCTTCCAGCGGGCATCCTGGGGTCCG GGCTGGCCCTCAAGGTGCAGGAGCAACACCGTCAGAAGCACTTTGAGAAAAGGAGGAAGCCAGCTGCTGA GCTCATTCAGGCTGCCTGGAGGTATTATGCTACCAACCCCAACAGGATTGACCTGGTGGCGACATGGAGA TTTTATGAATCAGTCGTCTCTTTTCCTTTCTTCAGGAAAGAACAGCTGGAGGCAGCATCCAGCCAAAAGC TGGGTCTCTTGGATCGGGTTCGCCTTTCTAATCCTCGTGGTAGCAATACTAAAGGAAAGCTATTTACCCC TCTGAATGTAGATGCCATAGAAGAAAGTCCTTCTAAAGAACCAAAGCCTGTTGGCTTAAACAATAAAGAG CGTTTCCGCACGGCCTTCCGCATGAAAGCCTACGCTTTCTGGCAGAGTTCTGAAGATGCCGGGACAGGTG ACCCCATGGCGGAAGACAGGGGCTATGGGAATGACTTCCCCATCGAAGACATGATCCCCACCCTGAAGGC CGCCATCCGAGCCGTCAGAATTCTACAATTCCGTCTCTATAAAAAAAAATTCAAGGAGACTTTGAGGCCT TACGATGTGAAGGATGTGATTGAGCAGTATTCTGCCGGGCATCTCGACATGCTTTCCAGGATAAAGTACC TTCAGACGAGAATAGATATGATTTTCACCCCTGGACCTCCCTCCACGCCAAAACACAAGAAGTCTCAGAA AGGGTCAGCATTCACCTTCCCATCCCAGCAATCTCCCAGGAATGAACCATATGTAGCCAGACCATCCACA TCAGAAATCGAAGACCAAAGCATGATGGGGAAGTTTGTAAAAGTTGAAAGACAGGTTCAGGACATGGGGA AGAAGCTGGACTTCCTCGTGGATATGCACATGCAACACATGGAACGGTTGCAGGTGCAGGTCACGGAGTA TTACCCAACCAAGGGCACCTCCTCGCCAGCTGAAGCAGAGAAGAAGGAGGACAACAGGTATTCCGATTTG AAAACCATCATCTGCAACTATTCTGAGACAGGCCCCCCGGAACCACCCTACAGCTTCCACCAGGTGACCA TTGACAAAGTCAGCCCCTATGGGTTTTTTGCACATGACCCTGTGAACCTGCCCCGAGGGGGACCCAGTTC TGGAAAGGTTCAGGCAACTCCTCCTTCCTCAGCAACAACGTATGTGGAGAGGCCCACGGTCCTGCCTATC TTGACTCTTCTCGACTCCCGAGTGAGCTGCCACTCCCAGGCTGACCTGCAGGGCCCCTACTCGGACCGAA TCTCCCCCCGGCAGAGACGTAGCATCACGCGAGACAGTGACACACCTCTGTCCCTGATGTCGGTCAACCA CGAGGAGCTGGAGAGGTCTCCAAGTGGCTTCAGCATCTCCCAGGACAGAGATGATTATGTGTTCGGCCCC AATGGGGGGTCGAGCTGGATGAGGGAGAAGCGGTACCTCGCCGAGGGTGAGACGGACACAGACACGGACC CCTTCACGCCCAGCGGCTCCATGCCTCTGTCGTCCACAGGGGATGGGATTTCTGATTCAGTATGGACCCC TTCCAATAAGCCCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234832 representing NM_001204824 Red=Cloning site Green=Tags(s) MKPAEHATMFLIVLGCLILAVLTTFKEYETVSGDWLLLLETFAIFIFGAEFALRIWAAGCCCRYKGWRGR LKFARKPLCMLDIFVLIASVPVVAVGNQGNVLATSLRSLRFLQILRMLRMDRRGGTWKLLGSAICAHSKE LITAWYIGFLTLILSSFLVYLVEKDVPEVDAQGEEMKEEFETYADALWWGLITLATIGYGDKTPKTWEGR LIAATFSLIGVSFFALPAGILGSGLALKVQEQHRQKHFEKRRKPAAELIQAAWRYYATNPNRIDLVATWR FYESVVSFPFFRKEQLEAASSQKLGLLDRVRLSNPRGSNTKGKLFTPLNVDAIEESPSKEPKPVGLNNKE RFRTAFRMKAYAFWQSSEDAGTGDPMAEDRGYGNDFPIEDMIPTLKAAIRAVRILQFRLYKKKFKETLRP YDVKDVIEQYSAGHLDMLSRIKYLQTRIDMIFTPGPPSTPKHKKSQKGSAFTFPSQQSPRNEPYVARPST SEIEDQSMMGKFVKVERQVQDMGKKLDFLVDMHMQHMERLQVQVTEYYPTKGTSSPAEAEKKEDNRYSDL KTIICNYSETGPPEPPYSFHQVTIDKVSPYGFFAHDPVNLPRGGPSSGKVQATPPSSATTYVERPTVLPI LTLLDSRVSCHSQADLQGPYSDRISPRQRRSITRDSDTPLSLMSVNHEELERSPSGFSISQDRDDYVFGP NGGSSWMREKRYLAEGETDTDTDPFTPSGSMPLSSTGDGISDSVWTPSNKPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204824
ORF Size	2256 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204824.1, NP_001191753.1
RefSeq Size	10717 bp
RefSeq ORF	2259 bp
Locus ID	3786
UniProt ID	O43525
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane
MW	85.3 kDa
Gene Summary	This gene encodes a protein that functions in the regulation of neuronal excitability. The encoded protein forms an M-channel by associating with the products of the related KCNQ2 or KCNQ5 genes, which both encode integral membrane proteins. M-channel currents are inhibited by M1 muscarinic acetylcholine receptors and are activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 2 (BFNC2), also known as epilepsy, benign neonatal type 2 (EBN2). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.