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SPSB1 (NM_025106) Human Tagged ORF Clone
CAT＃: RG203673
- TrueORF®
SPSB1 (tGFP-tagged) - Human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1)
ORF Plasmid: DDK
"NM_025106" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SSB-1; SSB1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203673 representing NM_025106
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCAGAAGGTCACTGGAGGGATCAAGACTGTGGACATGAGGGACCCCACGTACAGGCCCCTGAAGC AGGAGCTCCAGGGTCTGGATTACTGCAAGCCCACCCGGCTGGATCTGCTACTGGACATGCCCCCTGTGTC CTATGATGTCCAGCTGCTGCATTCATGGAACAACAACGACCGATCGCTCAATGTCTTTGTGAAGGAGGAC GACAAGCTCATCTTTCACCGGCATCCGGTGGCCCAGAGCACGGACGCTATCAGGGGCAAAGTCGGGTATA CCCGTGGGCTGCACGTGTGGCAGATCACGTGGGCCATGAGACAGCGGGGCACACACGCCGTGGTGGGGGT GGCGACGGCAGACGCCCCCCTGCACTCTGTCGGGTACACAACCCTCGTGGGGAATAACCACGAGTCCTGG GGCTGGGACTTGGGGCGCAACCGGCTCTACCACGATGGCAAGAACCAGCCAAGCAAAACATACCCAGCCT TTCTGGAACCAGATGAGACATTCATTGTCCCTGACTCCTTCCTGGTAGCCCTGGACATGGACGACGGGAC TCTGAGCTTCATTGTGGATGGACAGTACATGGGAGTGGCTTTTCGGGGACTCAAGGGCAAAAAACTGTAT CCTGTAGTGAGTGCCGTCTGGGGCCACTGTGAGATCCGAATGCGCTACTTGAACGGACTCGATCCCGAGC CGCTGCCGCTCATGGATTTGTGCCGTCGCTCGGTGCGCCTGGCCCTGGGGAGGGAGCGCCTGGGGGAGAT CCACACGCTGCCGCTGCCGGCTTCCCTCAAGGCCTACCTCCTCTACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203673 representing NM_025106
Red=Cloning site Green=Tags(s) MGQKVTGGIKTVDMRDPTYRPLKQELQGLDYCKPTRLDLLLDMPPVSYDVQLLHSWNNNDRSLNVFVKED DKLIFHRHPVAQSTDAIRGKVGYTRGLHVWQITWAMRQRGTHAVVGVATADAPLHSVGYTTLVGNNHESW GWDLGRNRLYHDGKNQPSKTYPAFLEPDETFIVPDSFLVALDMDDGTLSFIVDGQYMGVAFRGLKGKKLY PVVSAVWGHCEIRMRYLNGLDPEPLPLMDLCRRSVRLALGRERLGEIHTLPLPASLKAYLLYQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025106 |
| ORF Size | 819 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025106.4 |
| RefSeq Size | 3081 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 80176 |
| UniProt ID | Q96BD6 |
| Domains | SPRY |
| Protein Families | Druggable Genome |
| Gene Summary | Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820, PubMed:21199876). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203673 | SPSB1 (Myc-DDK-tagged)-Human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1) |
CNY 2,400.00 |
|
| RC203673L3 | Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203673L4 | Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mGFP tagged |
CNY 5,890.00 |
|
| SC110780 | SPSB1 (untagged)-Human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1) |
CNY 2,400.00 |
