HSD17B3 (NM_000197) Human Tagged ORF Clone
CAT＃: RG206558
- TrueORF®
HSD17B3 (tGFP-tagged) - Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3)
ORF Plasmid: DDK
"NM_000197" in other vectors (6)
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CNY 4000.00
CNY 4370.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EDH17B3; SDR12C2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206558 representing NM_000197
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACGTCCTGGAACAGTTCTTCATCCTCACAGGGCTGCTGGTGTGCCTGGCCTGCCTGGCGAAGT GCGTGAGATTCTCCAGATGTGTTTTACTGAACTACTGGAAAGTTTTGCCAAAGTCTTTCTTGCGGTCAAT GGGACAGTGGGCAGTGATCACTGGAGCAGGCGATGGAATTGGGAAAGCGTACTCGTTCGAGCTAGCAAAA CGTGGACTCAATGTTGTCCTTATTAGCCGGACGCTGGAAAAACTAGAGGCCATTGCCACAGAGATCGAGC GGACTACAGGGAGGAGTGTGAAGATTATACAAGCAGATTTTACAAAAGATGACATCTACGAGCATATTAA AGAAAAACTTGCAGGCTTAGAAATTGGAATTTTAGTCAACAATGTCGGAATGCTTCCAAACCTTCTCCCA AGCCATTTCCTGAACGCACCGGATGAAATCCAGAGCCTCATCCATTGTAACATCACCTCCGTAGTCAAGA TGACACAGCTAATTCTGAAACATATGGAATCAAGGCAGAAAGGTCTCATCCTGAACATTTCTTCTGGGAT AGCCCTGTTTCCTTGGCCTCTCTACTCCATGTACTCAGCTTCCAAGGCGTTTGTGTGCGCATTTTCCAAG GCCCTGCAAGAGGAATATAAAGCAAAAGAAGTCATCATCCAGGTGCTGACCCCATATGCTGTCTCGACTG CAATGACAAAGTATCTAAATACAAATGTGATAACCAAGACTGCTGATGAGTTTGTCAAAGAGTCATTGAA TTATGTCACAATTGGAGGTGAAACCTGTGGCTGCCTTGCCCATGAAATCTTGGCGGGCTTTCTGAGCCTG ATCCCGGCCTGGGCCTTCTACAGCGGTGCCTTCCAAAGGCTGCTCCTGACACACTATGTGGCATACCTGA AGCTCAACACCAAGGTCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206558 representing NM_000197
Red=Cloning site Green=Tags(s) MGDVLEQFFILTGLLVCLACLAKCVRFSRCVLLNYWKVLPKSFLRSMGQWAVITGAGDGIGKAYSFELAK RGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGMLPNLLP SHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLILNISSGIALFPWPLYSMYSASKAFVCAFSK ALQEEYKAKEVIIQVLTPYAVSTAMTKYLNTNVITKTADEFVKESLNYVTIGGETCGCLAHEILAGFLSL IPAWAFYSGAFQRLLLTHYVAYLKLNTKVR TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_000197 |
| ORF Size | 930 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000197.2 |
| RefSeq Size | 1134 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 3293 |
| UniProt ID | P37058 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Androgen and estrogen metabolism, Metabolic pathways |
| Gene Summary | This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206558 | HSD17B3 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3) |
CNY 2400.00 |
|
| RC206558L1 | Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3), Myc-DDK-tagged |
CNY 4800.00 |
|
| RC206558L2 | Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3), mGFP tagged |
CNY 5890.00 |
|
| RC206558L3 | Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC206558L4 | Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3), mGFP tagged |
CNY 5890.00 |
|
| SC300022 | HSD17B3 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3) |
CNY 2400.00 |
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