PGBD2 (NM_001017434) Human Tagged ORF Clone

CAT#: RG208978

  • TrueORF®

PGBD2 (tGFP-tagged) - Human piggyBac transposable element derived 2 (PGBD2), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001017434" in other vectors (4)

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CNY 4370.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG208978 representing NM_001017434
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTGCAATTTCCAGAAGCATGCACCCTTGGAAGAGTTCTACAGCTTTGGCGAGTCTATGTGTGAGT
ACTTTGGGCACCGGGGGTCCAAGCAGCTGCACAGGGGGAAGCCTGTGCGACTTGGCTACAAGATTTGGTG
TGGGACAACCAGCAGAGGCTACTTGGTGTGGTTTGAGCCCTCACAGGGCACACTGTTTACCAAGCCAGAC
AGGAGCTTGGATCTAGGAGGCAGTATGGTAATAAAATTTGTGGATGCGCTTCAGGAGCGTGGTTTTCTGC
CATATCACATATTTTTTGACAAGGTTTTCACAAGTGTTAAACTGATGTCCATTTTGAGGAAAAAGGGGGT
GAAAGCCACAGGAACTGTTCGTGAGTACAGGACTGAGCGATGTCCCCTAAAAGACCCCAAAGAACTGAAA
AAAATGAAGAGGGGTTCATTTGATTACAAAGTCGATGAGAGTGAGGAGATCATCGTGTGCCGCTGGCACG
ATAGCAGCGTGGTCAACATTTGCTCCAATGCTGTGGGCATAGAGCCAGTGAGGCTGACCAGTCGTCACTC
TGGAGCAGCTAAAACGCGGACTCAGGTCCACCAGCCATCACTGGTGAAGCTGTATCAGGAGAAGGTGGGT
GGCGTTGGTAGGATGGATCAGAATATTGCCAAGTACAAGGTGAAGATCCGAGGCATGAAGTGGTACTCAA
GCTTTATTGGCTATGTCATTGATGCTGCCCTCAACAATGCATGGCAGCTGCATAGAATCTGCTGCCAAGA
TGCCCAGGTGGACCTCCTTGCCTTCCGGAGATACATTGCCTGTGTGTATCTGGAGAGCAATGCTGACACA
ACATCTCAAGGGAGGCGAAGCAGGCGGTTGGAGACTGAGAGCCGCTTCGATATGATTGGGCACTGGATTA
TCCATCAGGACAAGAGGACCCGGTGTGCCCTCTGCCACTCACAGACCAACACCCGGTGTGAGAAGTGCCA
GAAGGGTGTCCATGCCAAATGCTTCAGGGAGTACCACATCCGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208978 representing NM_001017434
Red=Cloning site Green=Tags(s)

MNCNFQKHAPLEEFYSFGESMCEYFGHRGSKQLHRGKPVRLGYKIWCGTTSRGYLVWFEPSQGTLFTKPD
RSLDLGGSMVIKFVDALQERGFLPYHIFFDKVFTSVKLMSILRKKGVKATGTVREYRTERCPLKDPKELK
KMKRGSFDYKVDESEEIIVCRWHDSSVVNICSNAVGIEPVRLTSRHSGAAKTRTQVHQPSLVKLYQEKVG
GVGRMDQNIAKYKVKIRGMKWYSSFIGYVIDAALNNAWQLHRICCQDAQVDLLAFRRYIACVYLESNADT
TSQGRRSRRLETESRFDMIGHWIIHQDKRTRCALCHSQTNTRCEKCQKGVHAKCFREYHIR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_001017434
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001017434.1, NP_001017434.1
RefSeq Size 2035 bp
RefSeq ORF 1026 bp
Locus ID 267002
UniProt ID Q6P3X8
Gene Summary The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. The exact function of this gene is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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