PGBD2 (NM_001017434) Human Tagged ORF Clone
CAT#: RG208978
- TrueORF®
PGBD2 (tGFP-tagged) - Human piggyBac transposable element derived 2 (PGBD2), transcript variant 2
ORF Plasmid: DDK
"NM_001017434" in other vectors (4)
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CNY 4370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208978 representing NM_001017434
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGCAATTTCCAGAAGCATGCACCCTTGGAAGAGTTCTACAGCTTTGGCGAGTCTATGTGTGAGT ACTTTGGGCACCGGGGGTCCAAGCAGCTGCACAGGGGGAAGCCTGTGCGACTTGGCTACAAGATTTGGTG TGGGACAACCAGCAGAGGCTACTTGGTGTGGTTTGAGCCCTCACAGGGCACACTGTTTACCAAGCCAGAC AGGAGCTTGGATCTAGGAGGCAGTATGGTAATAAAATTTGTGGATGCGCTTCAGGAGCGTGGTTTTCTGC CATATCACATATTTTTTGACAAGGTTTTCACAAGTGTTAAACTGATGTCCATTTTGAGGAAAAAGGGGGT GAAAGCCACAGGAACTGTTCGTGAGTACAGGACTGAGCGATGTCCCCTAAAAGACCCCAAAGAACTGAAA AAAATGAAGAGGGGTTCATTTGATTACAAAGTCGATGAGAGTGAGGAGATCATCGTGTGCCGCTGGCACG ATAGCAGCGTGGTCAACATTTGCTCCAATGCTGTGGGCATAGAGCCAGTGAGGCTGACCAGTCGTCACTC TGGAGCAGCTAAAACGCGGACTCAGGTCCACCAGCCATCACTGGTGAAGCTGTATCAGGAGAAGGTGGGT GGCGTTGGTAGGATGGATCAGAATATTGCCAAGTACAAGGTGAAGATCCGAGGCATGAAGTGGTACTCAA GCTTTATTGGCTATGTCATTGATGCTGCCCTCAACAATGCATGGCAGCTGCATAGAATCTGCTGCCAAGA TGCCCAGGTGGACCTCCTTGCCTTCCGGAGATACATTGCCTGTGTGTATCTGGAGAGCAATGCTGACACA ACATCTCAAGGGAGGCGAAGCAGGCGGTTGGAGACTGAGAGCCGCTTCGATATGATTGGGCACTGGATTA TCCATCAGGACAAGAGGACCCGGTGTGCCCTCTGCCACTCACAGACCAACACCCGGTGTGAGAAGTGCCA GAAGGGTGTCCATGCCAAATGCTTCAGGGAGTACCACATCCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208978 representing NM_001017434
Red=Cloning site Green=Tags(s) MNCNFQKHAPLEEFYSFGESMCEYFGHRGSKQLHRGKPVRLGYKIWCGTTSRGYLVWFEPSQGTLFTKPD RSLDLGGSMVIKFVDALQERGFLPYHIFFDKVFTSVKLMSILRKKGVKATGTVREYRTERCPLKDPKELK KMKRGSFDYKVDESEEIIVCRWHDSSVVNICSNAVGIEPVRLTSRHSGAAKTRTQVHQPSLVKLYQEKVG GVGRMDQNIAKYKVKIRGMKWYSSFIGYVIDAALNNAWQLHRICCQDAQVDLLAFRRYIACVYLESNADT TSQGRRSRRLETESRFDMIGHWIIHQDKRTRCALCHSQTNTRCEKCQKGVHAKCFREYHIR TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001017434 |
ORF Size | 1023 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001017434.1, NP_001017434.1 |
RefSeq Size | 2035 bp |
RefSeq ORF | 1026 bp |
Locus ID | 267002 |
UniProt ID | Q6P3X8 |
Gene Summary | The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. The exact function of this gene is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208978 | PGBD2 (Myc-DDK-tagged)-Human piggyBac transposable element derived 2 (PGBD2), transcript variant 2 |
CNY 3656.00 |
|
RC208978L3 | Lenti-ORF clone of PGBD2 (Myc-DDK-tagged)-Human piggyBac transposable element derived 2 (PGBD2), transcript variant 2 |
CNY 5890.00 |
|
RC208978L4 | Lenti-ORF clone of PGBD2 (mGFP-tagged)-Human piggyBac transposable element derived 2 (PGBD2), transcript variant 2 |
CNY 5890.00 |
|
SC302053 | PGBD2 (untagged)-Human piggyBac transposable element derived 2 (PGBD2), transcript variant 2 |
CNY 7220.00 |