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KALRN (NM_003947) Human Tagged ORF Clone
CAT＃: RG216295
- TrueORF®
KALRN (tGFP-tagged) - Human kalirin, RhoGEF kinase (KALRN), transcript variant 2
ORF Plasmid: DDK
"NM_003947" in other vectors (4)
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CNY 18,340.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARHGEF24; CHD5; CHDS5; DUET; DUO; HAPIP; TRAD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216295 representing NM_003947
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGACCGCTTCTGGGACCAGTGGTATCTCTGGTATCTCCGCTTGCTCCGGCTGCTGGATCGAGGGT CTTTTCGGAATGATGGTTTGAAAGCTTCTGATGTCCTTCCTATCCTAAAGGAAAAGGTGGCCTTCGTGTC TGGGGGTCGTGATAAGCGAGGCGGACCCATCCTGACCTTCCCTGCTCGCAGCAATCATGACAGAATAAGA CAGGAAGACCTGCGGAAACTCGTGACGTATTTGGCCAGCGTGCCAAGTGAGGACGTGTGCAAACGTGGCT TCACTGTCATCATCGACATGCGGGGCTCCAAGTGGGACCTCATCAAGCCCCTCCTCAAAACGCTGCAGGA AGCCTTTCCAGCTGAGATCCATGTGGCCCTCATCATTAAACCCGACAACTTCTGGCAGAAACAGAAGACC AACTTTGGCAGCTCCAAATTCATCTTTGAGACGAGCATGGTATCTGTGGAGGGCCTCACAAAGCTGGTGG ACCCCTCCCAGCTGACGGAGGAGTTTGATGGCTCCCTGGACTACAACCATGAGGAGTGGATCGAACTGCG GCTCTCCCTGGAGGAGTTCTTCAACAGCGCCGTGCACCTGCTCTCGCGCCTCGAGGACCTCCAGGAGATG CTAGCCCGGAAGGAGTTTCCTGTGGATGTGGAGGGCTCTCGGCGGCTCATTGACGAACACACACAGCTCA AGAAAAAGGTGCTGAAGGCCCCTGTGGAGGAGCTGGACCGGGAGGGGCAGCGGCTGCTGCAGTGCATCCG CTGCAGCGACGGCTTCTCAGGACGCAACTGCATCCCGGGCAGTGCTGACTTCCAGAGCCTGGTGCCCAAG ATCACCAGTCTCCTGGACAAGCTGCACTCCACCCGGCAGCACCTGCACCAGATGTGGCATGTGCGCAAGC TCAAGCTGGACCAGTGCTTTCAGCTGCGGCTCTTCGAGCAGGATGCTGAGAAGATGTTTGACTGGATAAG CCACAACAAGGAGTTATTCCTCCAGAGCCACACGGAGATCGGAGTCAGCTACCAGTACGCCCTTGACCTC CAGACGCAGCACAATCACTTTGCCATGAACTCCATGAATGCCTATGTCAACATCAACCGCATCATGTCCG TGGCTTCCCGCCTCTCTGAGGCCGGTCATTATGCCTCACAACAAATCAAGCAGATCTCCACCCAGCTGGA CCAGGAGTGGAAGAGCTTCGCTGCTGCCCTGGATGAACGCAGCACCATCCTCGCCATGTCTGCTGTGTTC CACCAGAAGGCTGAGCAGTTCCTGTCGGGAGTGGATGCCTGGTGCAAGATGTGCAGTGAAGGTGGTCTGC CATCCGAGATGCAAGACCTAGAGCTGGCAATCCACCACCACCAGACCTTGTATGAGCAGGTGACCCAAGC CTACACAGAGGTCAGCCAGGATGGCAAAGCACTACTTGATGTGCTGCAGCGGCCCCTGAGCCCTGGGAAC TCCGAATCCCTCACGGCCACAGCCAACTACTCCAAGGCAGTGCACCAGGTGCTGGACGTGGTGCATGAGG TGTTACATCACCAGCGACGGCTGGAGAGCATCTGGCAGCACCGCAAGGTGCGGCTCCACCAGCGGCTGCA GCTCTGCGTCTTCCAGCAGGATGTACAGCAGGTGTTGGACTGGATTGAAAACCATGGTGAGGCCTTTCTC AGCAAACACACTGGAGTTGGGAAGTCCCTACATCGAGCCCGGGCCCTGCAGAAGAGGCATGATGACTTTG AAGAGGTGGCTCAGAATACGTACACCAATGCGGACAAGCTCCTAGAAGCAGCAGAGCAGTTGGCTCAGAC GGGGGAATGTGACCCCGAGGAGATCTACAAGGCAGCTCGACACCTGGAGGTGCGCATCCAAGACTTCGTG CGCAGGGTGGAGCAGCGGAAGCTTCTCCTGGACATGTCTGTTTCCTTCCACACACACACCAAAGAGTTGT GGACATGGATGGAAGACCTTCAGAAGGAGATGTTGGAGGATGTCTGTGCAGATTCTGTGGATGCAGTCCA GGAACTGATCAAGCAGTTCCAGCAGCAGCAGACCGCCACTCTAGATGCCACACTCAATGTCATCAAGGAA GGCGAAGACCTTATCCAGCAGCTCAGGTCAGCGCCTCCCTCCCTCGGGGAGCCCAGCGAGGCCAGGGACT CGGCTGTGTCCAACAACAAAACACCCCACAGCAGCTCCATCAGCCACATCGAGTCGGTCCTGCAGCAGCT TGATGATGCCCAGGTGCAGATGGAGGAGCTGTTCCACGAGCGGAAGATCAAGCTGGACATCTTCCTGCAA CTGCGCATCTTTGAGCAGTACACCATCGAGGTGACAGCAGAGCTAGACGCCTGGAATGAAGACTTGCTTC GGCAGATGAATGACTTCAACACAGAGGACCTAACCCTGGCAGAACAGCGGCTGCAGCGCCACACAGAACG GAAGCTAGCCATGAACAACATGACCTTTGAGGTTATCCAGCAGGGACAGGATCTGCACCAGTACATCACG GAGGTCCAGGCATCAGGAATTGAGTTGATCTGTGAAAAAGACATTGATCTGGCAGCCCAGGTGCAAGAGT TATTGGAATTTCTCCATGAGAAGCAGCATGAATTGGAGCTCAATGCAGAGCAGACTCATAAGCGGCTAGA GCAGTGCCTCCAATTACGTCACCTCCAGGCTGAAGTCAAACAGGTTCTGGGATGGATCCGCAATGGAGAG TCAATGCTCAACGCCAGCCTGGTCAATGCCAGCTCTTTGTCGGAAGCAGAGCAGCTGCAGCGGGAGCACG AGCAGTTCCAACTGGCCATCGAGTCCCTCTTTCATGCCACTTCCTTGCAGAAGACGCACCAGAGTGCCCT GCAGGTACAGCAGAAAGCCGAGGTGCTGCTCCAGGCCGGCCACTACGATGCCGATGCCATCCGGGAATGT GCTGAGAAGGTGGCCCTCCACTGGCAGCAGCTCATGCTGAAGATGGAAGACCGGCTAAAATTGGTCAATG CCTCTGTGGCCTTTTACAAAACTTCTGAACAGGTGTGTAGTGTCCTGGAGAGCTTAGAGCAAGAATACCG GAGAGATGAGGACTGGTGTGGTGGACGAGATAAGCTGGGGCCAGCAGCAGAGATCGACCATGTCATTCCC CTCATCAGCAAACATTTGGAACAAAAGGAGGCCTTTCTTAAGGCCTGCACCCTGGCTCGGCGGAATGCTG AGGTGTTTCTCAAGTACATCCACAGGAACAACGTCAGCATGCCCAGTGTCGCCAGCCACACTCGGGGACC CGAGCAACAAGTGAAAGCCATCCTGAGTGAGCTCCTGCAGAGGGAGAATCGCGTGCTGCATTTCTGGACC TTGAAGAAGCGGCGGTTAGACCAATGCCAGCAATATGTGGTGTTCGAGCGCAGCGCTAAGCAGGCGCTTG ACTGGATCCAAGAAACAGGTGAATTTTACCTCTCAACACATACCTCCACTGGAGAGACCACAGAGGAGAC TCAGGAACTGCTGAAAGAATATGGGGAATTCAGGGTGCCTGCCAAGCAAACAAAGGAGAAGGTGAAGCTT CTGATTCAGCTGGCCGATAGCTTTGTGGAAAAAGGCCACATTCATGCCACGGAGATAAGGAAATGGGTGA CCACGGTGGACAAGCACTACAGAGATTTCTCCCTGAGGATGGGAAAGTACCGATACTCACTGGAGAAAGC CCTAGGAGTCAACACAGAGGATAATAAGGACCTGGAGCTGGATATTATCCCAGCAAGCCTTTCGGATCGG GAGGTCAAGCTGCGGGACGCCAACCACGAAGTCAATGAAGAGAAGCGGAAGTCAGCCCGGAAGAAAGAAT TTATTATGGCTGAACTACTCCAGACAGAGAAGGCTTATGTAAGGGATTTGCATGAGTGCTTAGAGACCTA CCTGTGGGAAATGACCAGTGGTGTGGAGGAGATCCCCCCTGGGATCCTCAATAAAGAGCATATCATCTTT GGCAACATCCAAGAGATCTACGATTTCCATAACAACATCTTCCTCAAAGAGCTGGAGAAGTACGAGCAAC TGCCTGAGGATGTGGGACACTGCTTTGTTACCTGGGCAGACAAATTTCAGATGTATGTCACCTACTGTAA AAACAAGCCTGATTCCAACCAGCTTATCCTGGAGCATGCGGGCACCTTCTTTGATGAGATACAACAGCGG CATGGTCTGGCCAACTCCATCTCTTCCTACCTAATTAAGCCTGTCCAAAGGATCACCAAATATCAACTGC TCCTGAAGGAACTTTTAACTTGCTGTGAAGAAGGGAAAGGGGAGCTCAAGGATGGCCTGGAGGTGATGCT CAGTGTCCCAAAGAAAGCCAATGATGCCATGCATGTCAGCATGCTGGAAGGGTTCGACGAGAACCTGGAT GTGCAGGGGGAGTTGATTCTCCAGGATGCCTTTCAAGTGTGGGACCCGAAGTCGCTGATCCGGAAGGGGC GGGAGCGGCACTTGTTCCTCTTTGAGATCTCCTTGGTTTTTAGCAAGGAGATCAAAGATTCTTCAGGACA CACGAAATATGTTTACAAGAACAAGCTACTGACCTCAGAGCTGGGTGTGACCGAGCACGTGGAGGGCGAT CCCTGCAAATTCGCCTTGTGGTCTGGGCGCACCCCATCCTCAGACAATAAAACAGTGCTGAAAGCCTCCA ACATTGAAACCAAGCAGGAGTGGATCAAGAACATTCGAGAAGTGATTCAAGAAAGGATCATTCACCTGAA AGGAGCTTTAAAGGAGCCACTTCAGCTCCCCAAAACACCAGCCAAACAGAGGAACAATAGTAAGAGGGAT GGAGTGGAGGATATTGACAGCCAGGGGGATGGGAGCAGCCAACCAGACACCATCTCCATTGCTTCTAGGA CCTCTCAGAACACAGTGGACAGTGACAAGGATGGCAACCTTGTTCCTCGGTGGCACCTGGGACCTGGAGA TCCTTTCTCCACTTACGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216295 representing NM_003947
Red=Cloning site Green=Tags(s) MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIR QEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKT NFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEM LARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPK ITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQYALDL QTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVF HQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGN SESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFL SKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFV RRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEMLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKE GEDLIQQLRSAPPSLGEPSEARDSAVSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQ LRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIT EVQASGIELICEKDIDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGE SMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIREC AEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDHVIP LISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWT LKKRRLDQCQQYVVFERSAKQALDWIQETGEFYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKL LIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDR EVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIF GNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQR HGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLD VQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGD PCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPLQLPKTPAKQRNNSKRD GVEDIDSQGDGSSQPDTISIASRTSQNTVDSDKDGNLVPRWHLGPGDPFSTYV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003947 |
| ORF Size | 4989 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003947.6 |
| RefSeq Size | 6524 bp |
| RefSeq ORF | 4992 bp |
| Locus ID | 8997 |
| UniProt ID | O60229 |
| Domains | RhoGEF, SEC14, PH, spectrin |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. [provided by RefSeq, Apr 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216295 | KALRN (Myc-DDK-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 2 |
CNY 16,630.00 |
|
| RC216295L3 | Lenti-ORF clone of KALRN (Myc-DDK-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 2 |
CNY 18,530.00 |
|
| RC216295L4 | Lenti-ORF clone of KALRN (mGFP-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 2 |
CNY 18,530.00 |
|
| SC117652 | KALRN (untagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 2 |
CNY 11,384.00 |
