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LTBP4 (NM_003573) Human Tagged ORF Clone
CAT＃: RG216878
- TrueORF®
LTBP4 (tGFP-tagged) - Human latent transforming growth factor beta binding protein 4 (LTBP4), transcript variant 2
ORF Plasmid: DDK
"NM_003573" in other vectors (2)
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CNY 17,480.00
货期*
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规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARCL1C; LTBP-4; LTBP4L; LTBP4S |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216878 representing NM_003573
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACGTAAAAGCGTTGCTGTTTGTCGCTGCTGCCCGGGCCAGACGTCTAGGAGGAGCCGCTGCAT CCGAGTCCCTGGCTGTCTCCGAAGCCTTCTGCAGGGTCCGAAGCTGCCAGCCCAAAAAGTGTGCAGGCCC CCAGCGGTGCCTGAACCCAGTGCCTGCAGTGCCCAGTCCCAGCCCCAGCGTGAGGAAGAGACAGGTGTCC CTCAACTGGCAGCCACTGACGCTCCAGGAGGCCAGAGCTCTACTGAAGCGGCGGCGGCCCCGGGGGCCAG GGGGCCGGGGACTACTGAGAAGGAGGCCCCCACAGCGTGCCCCCGCTGGCAAGGCCCCGGTCCTGTGTCC CTTGATCTGTCACAATGGCGGTGTGTGCGTGAAGCCTGACCGCTGCCTCTGTCCCCCGGACTTCGCTGGC AAGTTCTGCCAGTTGCACTCCTCGGGCGCCCGGCCCCCGGCCCCGGCTGTACCAGGCCTCACCCGCTCCG TGTACACTATGCCACTGGCCAACCACCGCGACGACGAGCACGGCGTGGCATCTATGGTGAGCGTCCACGT GGAGCACCCGCAGGAGGCGTCGGTGGTGGTGCACCAGGTGGAGCGTGTGTCTGGCCCTTGGGAGGAGGCG GACGCTGAGGCGGTGGCGCGGGCGGAAGCGGCGGCGCGGGCGGAGGCGGCAGCGCCCTACACGGTGTTGG CACAGAGCGCGCCGCGGGAGGACGGCTACTCAGATGCCTCGGGCTTCGGTTACTGCTTTCGGGAGCTGCG CGGAGGCGAATGCGCGTCCCCGCTGCCCGGGCTCCGGACGCAGGAGGTCTGCTGCCGAGGGGCCGGCTTG GCCTGGGGCGTTCACGACTGTCAGCTGTGCTCCGAGCGCCTGGGGAACTCCGAAAGAGTGAGCGCCCCAG ATGGACCTTGTCCAACCGGCTTTGAAAGAGTTAATGGGTCCTGCGAAGATGTGGATGAGTGCGCGACTGG CGGGCGCTGCCAGCACGGCGAGTGTGCAAACACGCGCGGCGGGTACACGTGTGTGTGCCCCGACGGCTTT CTGCTCGACTCGTCCCGCAGCAGCTGCATCTCCCAACACGTGATCTCAGAGGCCAAAGGGCCCTGCTTCC GCGTGCTCCGCGACGGCGGCTGTTCGCTGCCCATTCTGCGGAACATCACTAAACAGATCTGCTGCTGCAG CCGCGTAGGCAAGGCCTGGGGCCGGGGCTGCCAGCTCTGCCCACCCTTCGGCTCAGAGGGTTTCCGGGAG ATCTGCCCGGCTGGTCCTGGTTACCACTACTCGGCCTCCGACCTCCGCTACAACACCAGACCCCTGGGCC AGGAGCCACCCCGAGTGTCACTCAGCCAGCCTCGTACCCTGCCAGCCACCTCTCGGCCATCTGCAGGCTT TCTGCCCACCCATCGCCTGGAGCCCCGGCCTGAACCCCGGCCCGATCCCCGGCCCGGCCCTGAGCTTCCC TTGCCCAGCATCCCTGCCTGGACTGGTCCTGAGATTCCTGAATCAGGTCCCTCCTCCGGCATGTGTCAGC GCAACCCCCAGGTCTGCGGCCCAGGACGCTGCATTTCCCGGCCCAGCGGCTACACCTGCGCTTGCGACTC TGGCTTCCGGCTCAGCCCCCAGGGCACCCGATGCATTGATGTGGACGAATGTCGCCGCGTGCCCCCGCCC TGTGCTCCCGGGCGCTGCGAGAACTCACCAGGCAGCTTCCGCTGCGTGTGCGGCCCGGGCTTCCGAGCCG GCCCACGGGCTGCGGAATGCCTGGATGTGGACGAGTGCCACCGCGTGCCGCCGCCGTGTGACCTCGGGCG CTGCGAGAACACGCCAGGCAGCTTCCTGTGCGTGTGCCCCGCCGGGTACCAGGCTGCACCGCACGGAGCC AGCTGCCAGGATGTGGATGAATGCACCCAGAGCCCAGGCCTGTGTGGCCGAGGGGCCTGCAAGAACCTGC CTGGCTCTTTCCGCTGTGTTTGCCCGGCTGGCTTCCGGGGCTCGGCGTGTGAAGAGGATGTGGATGAGTG TGCCCAGGAGCCGCCGCCCTGTGGGCCCGGCCGCTGTGACAACACGGCAGGCTCCTTTCACTGTGCCTGC CCTGCTGGCTTCCGCTCCCGAGGGCCCGGGGCCCCCTGCCAAGATGTGGATGAGTGTGCCCGAAGCCCCC CACCCTGCACCTACGGCCGGTGTGAGAACACAGAAGGCAGCTTCCAGTGTGTCTGCCCCATGGGCTTCCA ACCCAACACTGCTGGCTCCGAGTGCGAGGATGTGGATGAGTGTGAGAACCACCTCGCATGCCCTGGGCAG GAGTGTGTGAACTCGCCCGGCTCCTTCCAGTGCAGGACCTGTCCTTCTGGCCACCACCTGCACCGTGGCA GATGCACTGATGTGGACGAATGCAGTTCGGGTGCCCCTCCCTGTGGTCCCCACGGCCACTGCACTAACAC CGAAGGCTCCTTCCGCTGCAGCTGCGCGCCAGGCTACCGGGCGCCGTCGGGTCGGCCCGGGCCCTGCGCA GACGTGAACGAGTGCCTGGAGGGCGATTTCTGCTTCCCTCACGGCGAGTGCCTCAACACTGACGGCTCCT TTGCCTGTACTTGTGCCCCTGGCTACCGACCCGGACCCCGCGGAGCCTCTTGCCTCGACGTTGACGAGTG CAGCGAGGAGGACCTTTGCCAGAGCGGCATCTGTACCAACACCGACGGCTCCTTCGAGTGCATCTGTCCT CCGGGACACCGCGCTGGCCCGGACCTCGCCTCCTGCCTCGACGTGGACGAATGTCGCGAGCGAGGCCCAG CCCTGTGCGGGTCGCAGCGCTGTGAGAACTCTCCCGGCTCCTACCGCTGTGTCCGGGACTGCGATCCTGG GTACCACGCGGGCCCCGAGGGCACCTGTGACGATGTGGATGAGTGCCAAGAATATGGTCCCGAGATTTGT GGAGCCCAGCGTTGTGAGAACACCCCTGGCTCCTACCGCTGCACACCAGCCTGTGACCCTGGCTATCAGC CCACGCCAGGGGGCGGATGCCAGGATGTGGACGAATGCCGGAACCGGTCCTTCTGCGGTGCCCACGCCGT GTGCCAGAACCTGCCCGGCTCCTTCCAGTGCCTCTGTGACCAGGGTTACGAGGGGGCACGGGATGGGCGT CACTGCGTGGATGTGAACGAGTGTGAAACACTACAGGGTGTATGTGGAGCTGCCCTGTGTGAAAATGTCG AAGGCTCCTTCCTCTGTGTCTGCCCCAACAGCCCGGAAGAGTTTGACCCCATGACTGGACGCTGTGTTCC CCCACGAACTTCTGCTGGCACGTTCCCAGGCTCGCAGCCCCAGGCACCTGCTAGCCCCGTTCTGCCCGCC AGGCCACCTCCGCCACCCCTGCCCCGCCGACCCAGCACACCTAGGCAGGGCCCTGTGGGGAGTGGGCGCC GGGAGTGCTACTTTGACACAGCGGCCCCGGATGCATGTGACAACATCCTGGCTCGGAATGTGACATGGCA GGAGTGCTGCTGTACTGTGGGTGAGGGCTGGGGCAGCGGCTGCCGCATCCAGCAGTGCCCGGGCACCGAG ACAGCTGAGTACCAGTCATTGTGCCCTCACGGCCGGGGCTACCTGGCGCCCAGTGGAGACCTGAGCCTCC GGAGAGACGTGGACGAATGTCAGCTCTTCCGAGACCAGGTGTGCAAGAGTGGCGTGTGTGTGAACACGGC CCCGGGCTACTCATGCTATTGCAGCAACGGCTACTACTACCACACACAGCGGCTGGAGTGCATCGACAAT GACGAGTGCGCCGATGAGGAACCGGCCTGTGAGGGCGGCCGCTGTGTCAACACTGTGGGCTCTTATCACT GTACCTGCGAGCCCCCACTGGTGCTGGATGGCTCGCAGCGCCGCTGCGTCTCCAACGAGAGCCAGAGCCT CGATGACAATCTGGGAGTGTGCTGGCAGGAAGTGGGGGCTGACCTCGTGTGCAGCCACCCTCGGCTGGAC CGTCAGGCCACCTACACAGAGTGCTGCTGCCTGTATGGAGAGGCCTGGGGCATGGACTGCGCCCTCTGCC CTGCGCAGGACTCAGATGACTTCGAGGCCCTGTGCAATGTGCTACGCCCCCCCGCATATAGCCCCCCGCG ACCAGGTGGCTTTGGACTCCCCTACGAGTACGGCCCAGACTTAGGTCCACCTTACCAGGGCCTCCCATAT GGGCCTGAGTTGTACCCACCACCTGCGCTACCCTACGACCCCTACCCACCGCCACCTGGGCCCTTCGCCC GCCGGGAGGCTCCTTATGGGGCACCCCGCTTCGACATGCCAGACTTTGAGGACGATGGTGGCCCCTATGG CGAATCTGAGGCTCCTGCGCCACCTGGCCCGGGCACCCGCTGGCCCTATCGGTCCCGGGACACCCGCCGC TCCTTCCCAGAGCCCGAGGAGCCTCCTGAAGGTGGAAGCTATGCTGGTTCCCTGGCTGAGCCCTACGAGG AGCTGGAGGCGGAGGAGTGCGGGATCCTGGACGGCTGCACCAACGGCCGCTGCGTGCGCGTCCCCGAAGG CTTCACCTGCCGTTGCTTCGACGGCTACCGCCTGGACATGACCCGCATGGCCTGCGTTGACATCAACGAG TGTGATGAGGCCGAGGCTGCCTCCCCGCTGTGCGTCAACGCGCGTTGCCTCAACACGGATGGCTCCTTCC GCTGCATCTGCCGCCCGGGATTCGCACCCACGCACCAGCCGCACCACTGTGCGCCCGCACGGCCCCGGGC C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216878 representing NM_003573
Red=Cloning site Green=Tags(s) MGDVKALLFVAAARARRLGGAAASESLAVSEAFCRVRSCQPKKCAGPQRCLNPVPAVPSPSPSVRKRQVS LNWQPLTLQEARALLKRRRPRGPGGRGLLRRRPPQRAPAGKAPVLCPLICHNGGVCVKPDRCLCPPDFAG KFCQLHSSGARPPAPAVPGLTRSVYTMPLANHRDDEHGVASMVSVHVEHPQEASVVVHQVERVSGPWEEA DAEAVARAEAAARAEAAAPYTVLAQSAPREDGYSDASGFGYCFRELRGGECASPLPGLRTQEVCCRGAGL AWGVHDCQLCSERLGNSERVSAPDGPCPTGFERVNGSCEDVDECATGGRCQHGECANTRGGYTCVCPDGF LLDSSRSSCISQHVISEAKGPCFRVLRDGGCSLPILRNITKQICCCSRVGKAWGRGCQLCPPFGSEGFRE ICPAGPGYHYSASDLRYNTRPLGQEPPRVSLSQPRTLPATSRPSAGFLPTHRLEPRPEPRPDPRPGPELP LPSIPAWTGPEIPESGPSSGMCQRNPQVCGPGRCISRPSGYTCACDSGFRLSPQGTRCIDVDECRRVPPP CAPGRCENSPGSFRCVCGPGFRAGPRAAECLDVDECHRVPPPCDLGRCENTPGSFLCVCPAGYQAAPHGA SCQDVDECTQSPGLCGRGACKNLPGSFRCVCPAGFRGSACEEDVDECAQEPPPCGPGRCDNTAGSFHCAC PAGFRSRGPGAPCQDVDECARSPPPCTYGRCENTEGSFQCVCPMGFQPNTAGSECEDVDECENHLACPGQ ECVNSPGSFQCRTCPSGHHLHRGRCTDVDECSSGAPPCGPHGHCTNTEGSFRCSCAPGYRAPSGRPGPCA DVNECLEGDFCFPHGECLNTDGSFACTCAPGYRPGPRGASCLDVDECSEEDLCQSGICTNTDGSFECICP PGHRAGPDLASCLDVDECRERGPALCGSQRCENSPGSYRCVRDCDPGYHAGPEGTCDDVDECQEYGPEIC GAQRCENTPGSYRCTPACDPGYQPTPGGGCQDVDECRNRSFCGAHAVCQNLPGSFQCLCDQGYEGARDGR HCVDVNECETLQGVCGAALCENVEGSFLCVCPNSPEEFDPMTGRCVPPRTSAGTFPGSQPQAPASPVLPA RPPPPPLPRRPSTPRQGPVGSGRRECYFDTAAPDACDNILARNVTWQECCCTVGEGWGSGCRIQQCPGTE TAEYQSLCPHGRGYLAPSGDLSLRRDVDECQLFRDQVCKSGVCVNTAPGYSCYCSNGYYYHTQRLECIDN DECADEEPACEGGRCVNTVGSYHCTCEPPLVLDGSQRRCVSNESQSLDDNLGVCWQEVGADLVCSHPRLD RQATYTECCCLYGEAWGMDCALCPAQDSDDFEALCNVLRPPAYSPPRPGGFGLPYEYGPDLGPPYQGLPY GPELYPPPALPYDPYPPPPGPFARREAPYGAPRFDMPDFEDDGGPYGESEAPAPPGPGTRWPYRSRDTRR SFPEPEEPPEGGSYAGSLAEPYEELEAEECGILDGCTNGRCVRVPEGFTCRCFDGYRLDMTRMACVDINE CDEAEAASPLCVNARCLNTDGSFRCICRPGFAPTHQPHHCAPARPRA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003573 |
| ORF Size | 4761 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003573.2, NP_003564.2 |
| RefSeq Size | 5052 bp |
| RefSeq ORF | 4764 bp |
| Locus ID | 8425 |
| UniProt ID | Q8N2S1 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene binds transforming growth factor beta (TGFB) as it is secreted and targeted to the extracellular matrix. TGFB is biologically latent after secretion and insertion into the extracellular matrix, and sheds TGFB and other proteins upon activation. Defects in this gene may be a cause of cutis laxa and severe pulmonary, gastrointestinal, and urinary abnormalities. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
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