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SMARCA1 (NM_003069) Human Tagged ORF Clone
CAT＃: RG218308
- TrueORF®
SMARCA1 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (SMARCA1), transcript variant 1
ORF Plasmid: DDK
"NM_003069" in other vectors (4)
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CNY 11,590.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | hSNF2L; ISWI; NURF140; SNF2L; SNF2L1; SNF2LB; SNF2LT; SWI; SWI2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218308 representing NM_003069
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGGACACTGCCGCAGTGGCAGCCACCGTGGCAGCCGCGGATGCGACCGCCACTATCGTGGTCA TAGAGGACGAGCAGCCCGGGCCGTCCACCTCTCAGGAGGAGGGAGCGGCCGCCGCGGCCACCGAAGCCAC CGCGGCCACGGAGAAGGGCGAGAAGAAGAAGGAGAAAAACGTTTCTTCATTTCAACTCAAACTTGCTGCT AAAGCGCCTAAATCTGAAAAGGAAATGGACCCAGAATATGAAGAGAAAATGAAAGCCGACCGAGCAAAGA GATTTGAATTTTTACTGAAGCAGACAGAACTTTTTGCACATTTCATTCAGCCTTCAGCACAGAAATCTCC AACATCTCCACTGAACATGAAATTGGGACGTCCCCGAATAAAGAAAGATGAAAAGCAGAGCTTAATTTCT GCTGGAGACTACCGCCATAGGCGCACAGAGCAAGAAGAAGATGAAGAGCTACTGTCTGAGAGTCGGAAAA CATCTAATGTGTGTATTAGATTTGAGGTGTCACCTTCATATGTGAAAGGGGGGCCACTGAGAGATTATCA GATTCGAGGACTGAATTGGTTGATCTCTTTATATGAAAATGGAGTCAATGGCATTTTGGCTGATGAAATG GGCCTTGGGAAAACTTTACAAACAATTGCTTTGCTTGGTTACCTGAAACACTACCGAAATATTCCTGGAC CTCACATGGTTTTAGTTCCAAAGTCTACTTTACACAACTGGATGAATGAATTTAAACGATGGGTCCCATC TCTCCGTGTCATTTGTTTTGTCGGAGACAAGGATGCCAGAGCTGCTTTTATTCGTGATGAAATGATGCCA GGAGAGTGGGATGTTTGCGTTACTTCTTATGAGATGGTAATTAAAGAAAAATCTGTATTCAAAAAGTTTC ACTGGCGATACCTGGTCATTGATGAAGCTCACAGAATAAAGAATGAAAAATCTAAGCTTTCAGAGATTGT TCGTGAGTTCAAGTCGACTAACCGCTTGCTCCTAACTGGAACACCTTTGCAGAATAACCTGCATGAACTG TGGGCCTTACTCAACTTTTTATTGCCTGATGTCTTTAATTCTGCAGATGACTTTGATTCTTGGTTTGACA CTAAAAATTGTCTTGGTGATCAAAAACTCGTGGAAAGACTTCATGCAGTTTTAAAACCATTTTTGTTACG CCGTATAAAAACTGATGTAGAGAAGAGTCTGCCACCTAAAAAGGAAATAAAGATTTACTTGGGGCTGAGT AAGATGCAACGAGAATGGTATACAAAAATCCTGATGAAAGATATTGATGTTTTAAACTCTTCTGGCAAGA TGGACAAGATGCGACTCTTAAACATTCTGATGCAGCTTCGAAAGTGTTGTAATCATCCATATCTGTTTGA TGGTGCTGAACCTGGTCCACCTTATACCACTGATGAGCATATTGTCAGCAACAGTGGTAAAATGGTAGTT CTGGATAAACTATTGGCCAAACTCAAAGAACAGGGTTCAAGGGTTCTCATTTTCAGCCAGATGACTCGCT TGCTGGATATTTTGGAAGATTATTGCATGTGGCGTGGTTATGAGTATTGTCGACTGGATGGACAAACCCC GCATGAAGAAAGAGAGGATAAATTCCTAGAAGTGGAATTTCTGGGTCAAAGGGAAGCAATAGAGGCTTTT AATGCTCCTAATAGTAGCAAATTCATCTTTATGCTAAGTACCAGGGCTGGAGGTCTCGGAATTAACCTGG CAAGTGCTGATGTGGTTATACTATATGATTCAGACTGGAACCCACAGGTTGATCTACAAGCTATGGATCG AGCACATCGTATTGGTCAGAAGAAACCAGTACGTGTATTCCGTCTCATCACTGACAACACTGTTGAAGAG AGGATTGTAGAAAGAGCTGAGATAAAACTGAGACTCGATTCAATTGTTATACAACAAGGAAGACTCATTG ACCAACAGTCTAACAAGCTGGCAAAAGAGGAAATGTTACAAATGATACGGCATGGAGCCACCCATGTTTT TGCTTCTAAAGAGAGTGAGTTGACAGATGAAGACATTACAACTATTCTGGAAAGAGGGGAAAAGAAGACT GCAGAGATGAATGAACGCCTGCAAAAAATGGGAGAGTCTTCTCTAAGAAATTTTAGAATGGACATTGAAC AAAGTTTATACAAATTTGAGGGAGAAGATTATAGAGAAAAACAGAAGCTTGGCATGGTGGAATGGATTGA ACCTCCTAAACGAGAACGCAAAGCAAACTACGCAGTGGATGCCTACTTTAGAGAGGCTTTGCGTGTCAGC GAGCCAAAGATTCCAAAGGCTCCACGGCCTCCAAAACAGCCAAATGTTCAGGATTTTCAATTTTTCCCAC CACGCTTATTTGAGCTCCTGGAAAAGGAAATTCTTTATTATCGGAAGACAATAGGCTATAAGGTTCCAAG GAATCCTGATATCCCAAATCCAGCTCTGGCTCAAAGAGAAGAGCAAAAAAAGATTGATGGAGCTGAACCT CTTACACCAGAAGAGACTGAAGAAAAGGAAAAACTTCTCACACAAGGTTTCACAAACTGGACTAAACGAG ATTTTAACCAGTTTATTAAAGCTAATGAGAAATATGGAAGAGATGACATTGATAACATAGCTCGAGAGGT AGAGGGCAAATCCCCTGAGGAGGTCATGGAGTATTCAGCTGTATTTTGGGAACGTTGCAATGAATTACAG GACATTGAGAAAATTATGGCTCAAATTGAACGTGGAGAAGCAAGAATTCAACGAAGGATCAGTATCAAGA AAGCCCTGGATGCCAAAATTGCAAGATACAAGGCTCCATTTCATCAGTTGCGCATTCAGTATGGAACCAG CAAAGGAAAGAACTATACTGAGGAAGAAGATAGATTCTTGATTTGTATGTTACACAAAATGGGCTTTGAT AGAGAAAATGTATATGAAGAATTAAGACAGTGTGTACGAAATGCTCCCCAGTTTAGATTTGACTGGTTTA TCAAGTCTAGGACTGCCATGGAATTCCAGAGACGCTGTAACACTCTGATTTCATTGATTGAGAAAGAAAA TATGGAAATTGAGGAAAGAGAGAGAGCAGAAAAGAAGAAACGGGCAACTAAAACTCCAATGGTAAAATTT TCAGCATTTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218308 representing NM_003069
Red=Cloning site Green=Tags(s) MEQDTAAVAATVAAADATATIVVIEDEQPGPSTSQEEGAAAAATEATAATEKGEKKKEKNVSSFQLKLAA KAPKSEKEMDPEYEEKMKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLGRPRIKKDEKQSLIS AGDYRHRRTEQEEDEELLSESRKTSNVCIRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGILADEM GLGKTLQTIALLGYLKHYRNIPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDARAAFIRDEMMP GEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNNLHEL WALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIYLGLS KMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVSNSGKMVV LDKLLAKLKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYCRLDGQTPHEEREDKFLEVEFLGQREAIEAF NAPNSSKFIFMLSTRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEE RIVERAEIKLRLDSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFASKESELTDEDITTILERGEKKT AEMNERLQKMGESSLRNFRMDIEQSLYKFEGEDYREKQKLGMVEWIEPPKRERKANYAVDAYFREALRVS EPKIPKAPRPPKQPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPDIPNPALAQREEQKKIDGAEP LTPEETEEKEKLLTQGFTNWTKRDFNQFIKANEKYGRDDIDNIAREVEGKSPEEVMEYSAVFWERCNELQ DIEKIMAQIERGEARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFD RENVYEELRQCVRNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMVKF SAFS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003069 |
| ORF Size | 3162 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003069.5 |
| RefSeq Size | 4102 bp |
| RefSeq ORF | 3165 bp |
| Locus ID | 6594 |
| UniProt ID | P28370 |
| Domains | SNF2_N, myb_DNA-binding, DEAD, helicase_C |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218308 | SMARCA1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (SMARCA1), transcript variant 1 |
CNY 7,336.00 |
|
| RC218308L3 | Lenti-ORF clone of SMARCA1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (SMARCA1), transcript variant 1 |
CNY 12,450.00 |
|
| RC218308L4 | Lenti-ORF clone of SMARCA1 (mGFP-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (SMARCA1), transcript variant 1 |
CNY 12,450.00 |
|
| SC309990 | SMARCA1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (SMARCA1), transcript variant 1 |
CNY 7,344.00 |
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