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OGA (NM_001142434) Human Tagged ORF Clone
CAT＃: RG226720
- TrueORF®
MGEA5 (tGFP-tagged) - Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2
ORF Plasmid: DDK
"NM_001142434" in other vectors (4)
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CNY 10,170.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MEA5; MGEA5; NCOAT |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226720 representing NM_001142434
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGAAGGAGAGTCAAGCGACGTTGGAGGAGCGGGAGAGCGAGCTCAGCTCCAACCCTGCCGCCT CTGCGGGGGCATCGCTGGAGCCGCCGGCAGCTCCGGCACCCGGAGAAGACAACCCCGCCGGGGCTGGGGG AGCGGCGGTGGCCGGGGCTGCAGGAGGGGCTCGGCGGTTCCTCTGCGGTGTGGTGGAAGGATTTTATGGA AGACCTTGGGTTATGGAACAGAGAAAAGAACTCTTTAGAAGGCTCCAGAAATGGGAATTAAATACATACT TGTATGCCCCAAAAGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAGGAAGCTGA GCAACTTATGACTCTCATCTCTGCTGCACGAGAATATGAGATAGAGTTCATCTATGCGATCTCACCTGGA TTGGATATCACTTTTTCTAACCCCAAGGAAGTATCCACATTGAAACGTAAATTGGACCAGGTTTCTCAGT TTGGGTGCAGATCATTTGCTTTGCTTTTTGATGATATAGACCATAATATGTGTGCAGCAGACAAAGAGGT ATTCAGTTCTTTTGCTCATGCCCAAGTCTCCATCACAAATGAAATCTATCAGTACCTAGGAGAGCCAGAA ACTTTCCTCTTCTGTCCCACAGAATACTGTGGCACTTTCTGTTATCCAAATGTGTCTCAGTCTCCATATT TAAGGACTGTGGGTGAAAAGCTTCTACCTGGAATTGAAGTGCTTTGGACAGGTCCCAAAGTTGTTTCTAA AGAAATTCCAGTAGAGTCCATCGAAGAGGTTTCTAAGATTATTAAGAGAGCTCCAGTAATCTGGGATAAC ATTCATGCTAATGATTATGATCAGAAGAGACTGTTTCTGGGCCCGTACAAAGGAAGATCCACAGAACTCA TCCCACGGTTAAAAGGAGTCCTCACTAATCCAAATTGTGAATTTGAAGCCAACTACGTTGCTATCCACAC CCTTGCCACCTGGTACAAATCAAACATGAATGGAGTGAGAAAAGATGTAGTGATGAGTAGGCAAGTTGCA CACAGTGGAGCTAAAGCAAGTGTAGTTGATGGGACTCCTTTAGTTGCAGCACCCTCTTTAAATGCCACAA CCGTAGTAACAACAGTTTATCAGGAGCCCATTATGAGCCAGGGAGCAGCCTTGAGTGGTGAGCCTACTAC TCTGACCAAGGAAGAAGAAAAGAAACAGCCTGATGAAGAACCCATGGACATGGTGGTGGAAAAACAAGAA GAAACGGACCACAAGAATGACAATCAAATACTGAGTGAAATTGTTGAAGCGAAAATGGCAGAGGAATTGA AACCAATGGACACTGATAAAGAGAGCATAGCTGAATCAAAATCCCCAGAGATGTCCATGCAAGAAGATTG TATTAGTGACATTGCCCCCATGCAAACTGATGAACAGACAAACAAGGAGCAGTTTGTGCCAGGTCCAAAT GAAAAGCCTTTGTACACTGCGGAACCAGTGACCCTGGAGGATTTGCAGTTACTTGCTGATCTATTCTACC TTCCTTACGAGCATGGACCCAAAGGAGCACAGATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAG TGTTGTCAGTGTCAATTGCAAAGGAAAAGACTCTGAAAAAATTGAAGAATGGCGGTCACGAGCAGCCAAG TTTGAAGAGATGTGTGGACTAGTGATGGGAATGTTCACTCGGCTCTCCAATTGTGCCAACAGGACAATTC TTTATGACATGTACTCCTATGTTTGGGATATCAAGAGTATAATGTCTATGGTGAAGTCTTTTGTACAGTG GTTAGGGTGTCGTAGTCATTCTTCAGCACAATTCTTAATTGGAGACCAAGAACCCTGGGCCTTTAGAGGT GGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGGGCAAATGATCTCTTTTTTCAGCCACCTC CACTGACTCCTACCTCCAAAGTTTATACTATCAGACCTTATTTTCCTAAGGATGAGGCATCCGTGTACAA GATTTGCAGAGAAATGTATGACGATGGAGTGGGTTTACCCTTTCAAAGTCAGCCTGATCTTATTGGAGAC AAGTTAGTAGGAGGGCTGCTTTCCCTCAGCCTGGATTACTGCTTTGTCCTAGAAGATGAAGATGGCATAT GTGGTTATGCCTTGGGCACTGTAGATGTGACCCCCTTTATTAAAAAATGTAAAATTTCCTGGATCCCCTT CATGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTGAGGCTGAGAAAATAATGTTGAGT TTCCATGAAGAACAGGAAGTACTGCCAGAAACTTTCCTTGCTAATTTCCCTTCTCTGATAAAGATGGACA TTCACAAAAAAGTAACTGACCCAAGTGTGGCCAAAAGCATGATGGCTTGCCTCCTGTCTTCACTGAAGGC TAATGGCTCCCGGGGAGCTTTCTGTGAAGTGAGACCAGATGATAAAAGAATTCTGGAATTTTACAGCAAG TTAGGATGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTTATACTTGGTCGGAGCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226720 representing NM_001142434
Red=Cloning site Green=Tags(s) MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYG RPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPG LDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPE TFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDN IHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMSRQVA HSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQE ETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPN EKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAK FEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRG GLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGD KLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLS FHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSK LGCFEIAKMEGFPKDVVILGRSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142434 |
| ORF Size | 2589 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142434.1, NP_001135906.1 |
| RefSeq Size | 5061 bp |
| RefSeq ORF | 2592 bp |
| Locus ID | 10724 |
| UniProt ID | O60502 |
| Gene Summary | The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226720 | MGEA5 (Myc-DDK-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2 |
CNY 9,220.00 |
|
| RC226720L3 | Lenti-ORF clone of MGEA5 (Myc-DDK-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2 |
CNY 11,120.00 |
|
| RC226720L4 | Lenti-ORF clone of MGEA5 (mGFP-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2 |
CNY 11,120.00 |
|
| SC326262 | MGEA5 (untagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2 |
CNY 6,328.00 |
