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Slingshot homolog 1 (SSH1) (NM_001161331) Human Tagged ORF Clone
CAT＃: RG228546
- TrueORF®
SSH1 (tGFP-tagged) - Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 3
ORF Plasmid: DDK
"NM_001161331" in other vectors (4)
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CNY 8,170.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SSH1L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228546 representing NM_001161331
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGAGCTCGGAGGGCAGTTGTAGGGAGCGTTAGAGATGTGAGCACTGCAGCCACGAACCTCTTTT ATTTTACCGACTTCTGCATCTTCCTTCAGCCAACCCACTGCTTCTGCTGTCCAGAGGTTTCCAGCTCAAA TTACTTAAGTGAGAGCTTTTTCATGGTGAAAGGCGCAGCCCTCTTCTTACAACAGGGAAGCAGCCCTCAA GGCCAGCGGAGTCTTCAGCACCCCCACAAGCATGCAGGTGATCTGCCTCAACATCTTCAGGTGATGATCA ACCTTCTGCGTTGCGAAGACAGAATCAAGCTGGCAGTGCGCCTGGAGAGCGCCTGGGCGGACCGGGTCCG GTACATGGTGGTGGTGTACAGCAGCGGGCGCCAGGACACCGAGGAGAATATCTTGCTGGGAGTGGACTTT TCCAGTAAGGAAAGTAAAAGCTGCACCATTGGGATGGTTCTCCGACTGTGGAGCGACACGAAAATCCACC TTGATGGAGATGGTGGGTTCAGCGTGAGCACAGCAGGAAGGATGCACATATTTAAGCCTGTGTCTGTCCA GGCCATGTGGTCTGCCCTGCAGGTGCTTCACAAGGCCTGCGAAGTGGCCCGGAGGCACAACTACTTCCCC GGGGGTGTAGCTCTCATCTGGGCTACCTACTATGAGAGCTGCATCAGCTCCGAGCAGAGCTGCATCAACG AGTGGAACGCCATGCAGGACCTGGAGTCTACGCGGCCCGACTCCCCCGCGCTATTTGTGGACAAGCCCAC TGAAGGGGAAAGGACCGAGCGCCTCATCAAAGCCAAGCTCCGAAGCATCATGATGAGCCAGGATCTAGAA AATGTGACTTCCAAAGAGATTCGTAATGAATTAGAGAAACAGATGAATTGTAACTTGAAGGAACTCAAGG AATTTATAGACAATGAGATGCTACTTATCTTGGGACAGATGGACAAGCCCTCCCTTATCTTCGATCATCT TTATCTCGGCTCTGAATGGAATGCATCCAATCTGGAGGAACTGCAGGGCTCAGGGGTTGATTACATTTTA AATGTTACCAGAGAAATCGATAATTTTTTTCCTGGCTTATTTGCATATCATAACATCCGAGTCTACGATG AAGAGACCACAGACCTCCTCGCCCACTGGAATGAAGCGTATCATTTTATAAACAAAGCGAAGAGGAACCA TTCCAAGTGCCTGGTGCATTGCAAAATGGGCGTGAGTCGCTCGGCCTCCACAGTCATAGCCTATGCAATG AAGGAATTCGGCTGGCCTCTGGAAAAAGCATATAACTATGTAAAGCAGAAGCGCAGCATCACGCGCCCCA ACGCGGGCTTTATGAGGCAGCTGTCTGAGTATGAAGGCATCTTGGATGCAAGCAAACAGCGGCACAACAA GCTGTGGCGTCAGCAGACAGACAGCAGCCTCCAGCAGCCTGTGGATGACCCTGCAGGACCTGGCGACTTC TTGCCAGAGACCCCAGATGGCACCCCGGAAAGCCAGCTGCCCTTCTTGGATGATGCCGCCCAGCCCGGCT TAGGGCCCCCCCTCCCCTGCTGTTTCCGGCGACTCTCAGACCCCCTTCTGCCTTCCCCTGAGGATGAAAC TGGCAGCTTGGTCCACCTGGAGGATCCGGAGAGGGAGGCTCTGTTGGAGGAAGCTGCTCCACCTGCAGAG GTGCACAGGCCGGCCAGACAGCCCCAGCAAGGTTCCGGACTCTGTGAGAAGGATGTGAAGAAGAAACTAG AGTTTGGGAGTCCCAAAGGTCGGAGCGGCTCCTTGCTGCAGGTGGAGGAGACGGAAAGGGAGGAGGGCCT GGGAGCAGGGAGGTGGGGGCAGCTTCCAACCCAGCTCGATCAAAACCTGCTCAACTCGGAGAACCTAAAC AACAACAGCAAGAGGAGCTGTCCCAACGGCATGGAGGTAGGCAGAGCCCGGCCTGCAGGGTGGCACACCC CATCCCTTCCATCCCACTCTAATTGGCCTACCTCAGCCTCTGTAGTAGGGACTACAGGCACCCGCCACCA CACCCAGCTGATTTTTTTCTATTGTCTCCTCTGGGCCCCCAGCTCCCATCTCCAGGGACCTGAGGGTTCT TTCACAGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228546 representing NM_001161331
Red=Cloning site Green=Tags(s) MARARRAVVGSVRDVSTAATNLFYFTDFCIFLQPTHCFCCPEVSSSNYLSESFFMVKGAALFLQQGSSPQ GQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESAWADRVRYMVVVYSSGRQDTEENILLGVDF SSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFP GGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLE NVTSKEIRNELEKQMNCNLKELKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYIL NVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAM KEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQTDSSLQQPVDDPAGPGDF LPETPDGTPESQLPFLDDAAQPGLGPPLPCCFRRLSDPLLPSPEDETGSLVHLEDPEREALLEEAAPPAE VHRPARQPQQGSGLCEKDVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGRWGQLPTQLDQNLLNSENLN NNSKRSCPNGMEVGRARPAGWHTPSLPSHSNWPTSASVVGTTGTRHHTQLIFFYCLLWAPSSHLQGPEGS FTG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161331 |
| ORF Size | 2109 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001161331.1, NP_001154803.1 |
| RefSeq Size | 2459 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 54434 |
| UniProt ID | Q8WYL5 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Regulation of actin cytoskeleton |
| Gene Summary | The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228546 | SSH1 (Myc-DDK-tagged)-Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 3 |
CNY 7,410.00 |
|
| RC228546L3 | Lenti-ORF clone of SSH1 (Myc-DDK-tagged)-Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 3 |
CNY 9,310.00 |
|
| RC228546L4 | Lenti-ORF clone of SSH1 (mGFP-tagged)-Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 3 |
CNY 9,310.00 |
|
| SC327181 | SSH1 (untagged)-Human slingshot homolog 1 (Drosophila) (SSH1) transcript variant 3 |
CNY 13,590.00 |
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