LY75 (LY75-CD302) (NM_001198760) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	CD205; CLEC13B; DEC-205; gp200-MR6; Ly-75; LY75
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG231492 representing NM_001198760 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACAGGCTGGGCGACCCCTCGCCGCCCGGCGGGGCTCCTCATGCTGCTCTTCTGGTTCTTCGATC TCGCGGAGCCCTCTGGCCGCGCAGCTAATGACCCCTTCACCATCGTCCATGGAAATACGGGCAAGTGCAT CAAGCCAGTGTATGGCTGGATAGTAGCAGACGACTGTGATGAAACTGAGGACAAGTTATGGAAGTGGGTG TCCCAGCATCGGCTCTTTCATTTGCACTCCCAAAAGTGCCTTGGCCTCGATATTACCAAATCGGTAAATG AGCTGAGAATGTTCAGCTGTGACTCCAGTGCCATGCTGTGGTGGAAATGTGAGCACCACTCTCTGTACGG AGCTGCCCGGTACCGGCTGGCTCTGAAGGATGGACATGGCACAGCAATCTCAAATGCATCTGATGTCTGG AAGAAAGGAGGCTCAGAGGAAAGCCTTTGTGACCAGCCTTATCATGAGATCTATACCAGAGATGGGAACT CTTATGGGAGACCTTGTGAATTTCCATTCTTAATTGATGGGACCTGGCATCATGATTGCATTCTTGATGA AGATCATAGTGGGCCATGGTGTGCCACCACCTTAAATTATGAATATGACCGAAAGTGGGGCATCTGCTTA AAGCCTGAAAACGGTTGTGAAGATAATTGGGAAAAGAACGAGCAGTTTGGAAGTTGCTACCAATTTAATA CTCAGACGGCTCTTTCTTGGAAAGAAGCTTATGTTTCATGTCAGAATCAAGGAGCTGATTTACTGAGCAT CAACAGTGCTGCTGAATTAACTTACCTTAAAGAAAAAGAAGGCATTGCTAAGATTTTCTGGATTGGTTTA AATCAGCTATACTCTGCTAGAGGCTGGGAATGGTCAGACCACAAACCATTAAACTTTCTCAACTGGGATC CAGACAGGCCCAGTGCACCTACTATAGGTGGCTCCAGCTGTGCAAGAATGGATGCTGAGTCTGGTCTGTG GCAGAGCTTTTCCTGTGAAGCTCAACTGCCCTATGTCTGCAGGAAACCATTAAATAATACAGTGGAGTTA ACAGATGTCTGGACATACTCAGATACCCGCTGTGATGCAGGCTGGCTGCCAAATAATGGATTTTGCTATC TGCTGGTAAATGAAAGTAATTCCTGGGATAAGGCACATGCGAAATGCAAAGCCTTCAGTAGTGACCTAAT CAGCATTCATTCTCTAGCAGATGTGGAGGTGGTTGTCACAAAACTCCATAATGAGGATATCAAAGAAGAA GTGTGGATAGGCCTTAAGAACATAAACATACCAACTTTATTTCAGTGGTCAGATGGTACTGAAGTTACTC TAACATATTGGGATGAGAATGAGCCAAATGTTCCCTACAATAAGACGCCCAACTGTGTTTCCTACTTAGG AGAGCTAGGTCAGTGGAAAGTCCAATCATGTGAGGAGAAACTAAAATATGTATGCAAGAGAAAGGGAGAA AAACTGAATGACGCAAGTTCTGATAAGATGTGTCCTCCAGATGAGGGCTGGAAGAGACATGGAGAAACCT GTTACAAGATTTATGAGGATGAGGTCCCTTTTGGAACAAACTGCAATCTGACTATCACTAGCAGATTTGA GCAAGAATACCTAAATGATTTGATGAAAAAGTATGATAAATCTCTAAGAAAATACTTCTGGACTGGCCTG AGAGATGTAGATTCTTGTGGAGAGTATAACTGGGCAACTGTTGGTGGAAGAAGGCGGGCTGTAACCTTTT CCAACTGGAATTTTCTTGAGCCAGCTTCCCCGGGCGGCTGCGTGGCTATGTCTACTGGAAAGTCTGTTGG AAAGTGGGAGGTGAAGGACTGCAGAAGCTTCAAAGCACTTTCAATTTGCAAGAAAATGAGTGGACCCCTT GGGCCTGAAGAAGCATCCCCTAAGCCTGATGACCCCTGTCCTGAAGGCTGGCAGAGTTTCCCCGCAAGTC TTTCTTGTTATAAGGTATTCCATGCAGAAAGAATTGTAAGAAAGAGGAACTGGGAAGAAGCTGAACGATT CTGCCAAGCCCTTGGAGCACACCTTTCTAGCTTCAGCCATGTGGATGAAATAAAGGAATTTCTTCACTTT TTAACGGACCAGTTCAGTGGCCAGCATTGGCTGTGGATTGGTTTGAATAAAAGGAGCCCAGATTTACAAG GATCCTGGCAATGGAGTGATCGTACACCAGTGTCTACTATTATCATGCCAAATGAGTTTCAGCAGGATTA TGACATCAGAGACTGTGCTGCTGTCAAGGTATTTCATAGGCCATGGCGAAGAGGCTGGCATTTCTATGAT GATAGAGAATTTATTTATTTGAGGCCTTTTGCTTGTGATACAAAACTTGAATGGGTGTGCCAAATTCCAA AAGGCCGTACTCCAAAAACACCAGACTGGTACAATCCAGACCGTGCTGGAATTCATGGACCTCCACTTAT AATTGAAGGAAGTGAATATTGGTTTGTTGCTGATCTTCACCTAAACTATGAAGAAGCCGTCCTGTACTGT GCCAGCAATCACAGCTTTCTTGCAACTATAACATCTTTTGTGGGACTAAAAGCCATCAAAAACAAAATAG CAAATATATCTGGTGATGGACAGAAGTGGTGGATAAGAATTAGCGAGTGGCCAATAGATGATCATTTTAC ATACTCACGATATCCATGGCACCGCTTTCCTGTGACATTTGGAGAGGAATGCTTGTACATGTCTGCCAAG ACTTGGCTTATCGACTTAGGTAAACCAACAGACTGTAGTACCAAGTTGCCCTTCATCTGTGAAAAATATA ATGTTTCTTCGTTAGAGAAATACAGCCCAGATTCTGCAGCTAAAGTGCAATGTTCTGAGCAATGGATTCC TTTTCAGAATAAGTGTTTTCTAAAGATCAAACCCGTGTCTCTCACATTTTCTCAAGCAAGCGATACCTGT CACTCCTATGGTGGCACCCTTCCTTCAGTGTTGAGCCAGATTGAACAAGACTTTATTACATCCTTGCTTC CGGATATGGAAGCTACTTTATGGATTGGTTTGCGCTGGACTGCCTATGAAAAGATAAACAAATGGACAGA TAACAGAGAGCTGACGTACAGTAACTTTCACCCATTATTGGTTAGTGGGAGGCTGAGAATACCAGAAAAT TTTTTTGAGGAAGAGTCTCGCTACCACTGTGCCCTAATACTCAACCTCCAAAAATCACCGTTTACTGGGA CGTGGAATTTTACATCCTGCAGTGAACGCCACTTTGTGTCTCTCTGTCAGAAATATTCAGAAGTTAAAAG CAGACAGACGTTGCAGAATGCTTCAGAAACTGTAAAGTATCTAAATAATCTGTACAAAATAATCCCAAAG ACTCTGACTTGGCACAGTGCTAAAAGGGAGTGTCTGAAAAGTAACATGCAGCTGGTGAGCATCACGGACC CTTACCAGCAGGCATTCCTCAGTGTGCAGGCGCTCCTTCACAACTCTTCCTTATGGATCGGACTCTTCAG TCAAGATGATGAACTCAACTTTGGTTGGTCAGATGGGAAACGTCTTCATTTTAGTCGCTGGGCTGAAACT AATGGGCAACTCGAAGACTGTGTAGTATTAGACACTGATGGATTCTGGAAAACAGTTGATTGCAATGACA ATCAACCAGGTGCTATTTGCTACTATTCAGGAAATGAGACTGAAAAAGAGGTCAAACCAGTTGACAGTGT TAAATGTCCATCTCCTGTTCTAAATACTCCGTGGATACCATTTCAGAACTGTTGCTACAATTTCATAATA ACAAAGAATAGGCATATGGCAACAACACAGGATGAAGTTCATACTAAATGCCAGAAACTGAATCCAAAAT CACATATTCTGAGTATTCGAGATGAAAAGGAGAATAACTTTGTTCTTGAGCAACTGCTGTACTTCAATTA TATGGCTTCATGGGTCATGTTAGGAATAACTTATAGAAATAAGTCTCTTATGTGGTTTGATAAGACCCCA CTGTCATATACACATTGGAGAGCAGGAAGACCAACTATAAAAAATGAGAAGTTTTTGGCTGGTTTAAGTA CTGACGGCTTCTGGGATATTCAAACCTTTAAAGTTATTGAAGAAGCAGTTTATTTTCACCAGCACAGCAT TCTTGCTTGTAAAATTGAAATGGTTGACTACAAAGAAGAATATAATACTACACTGCCACAGTTTATGCCA TATGAAGATGGTATTTACAGTGTTATTCAAAAAAAGGTAACATGGTATGAAGCATTAAACATGTGTTCTC AAAGTGGAGGTCACTTGGCAAGCGTTCACAACCAAAATGGCCAGCTCTTTCTGGAAGATATTGTAAAACG TGATGGATTTCCACTATGGGTTGGGCTCTCAAGTCATGATGGAAGTGAATCAAGTTTTGAATGGTCTGAT GGTAGTACATTTGACTATATCCCATGGAAAGGCCAAACATCTCCTGGAAATTGTGTTCTCTTGGATCCAA AAGGAACTTGGAAACATGAAAAATGCAACTCTGTTAAGGATGGTGCTATTTGTTATAAACCTACAAAATC TAAAAAGCTGTCCCGTCTTACATATTCATCAAGATGTCCAGCAGCAAAAGAGAATGGGTCACGGTGGATC CAGTACAAGGGTCACTGTTACAAGTCTGATCAGGCATTGCACAGTTTTTCAGAGGCCAAAAAATTGTGTT CAAAACATGATCACTCTGCAACTATCGTTTCCATAAAAGATGAAGATGAGAATAAATTTGTGAGCAGACT GATGAGGGAAAATAATAACATTACCATGAGAGTTTGGCTTGGATTATCTCAACATTCTGTTGACTGTCCT TCATCTACTTGGATTCAGTTCCAAGACAGTTGTTACATTTTTCTCCAAGAAGCCATCAAAGTAGAAAGCA TAGAGGATGTCAGAAATCAGTGTACTGACCATGGAGCGGACATGATAAGCATACATAATGAAGAAGAAAA TGCTTTTATACTGGATACTTTGAAAAAGCAATGGAAAGGCCCAGATGATATCCTACTAGGCATGTTTTAT GACACAGATGATGCGAGTTTCAAGTGGTTTGATAATTCAAATATGACATTTGATAAGTGGACAGACCAAG ATGATGATGAGGATTTAGTTGACACCTGTGCTTTTCTGCACATCAAGACAGGTGAATGGAAAAAAGGAAA TTGTGAAGTTTCTTCTGTGGAAGGAACACTATGCAAAACAGCTATCCCATACAAAAGGAAATATTTATCA GATAACCACATTTTAATATCAGCATTGGTGATTGCTAGCACGGTAATTTTGACAGTTTTGGGAGCAATCA TTTGGTTCCTGTACAAAAAACATTCTGATTCTCGTTTCACCACAGTTTTTTCAACCGCACCCCAATCACC TTATAATGAAGACTGTGTTTTGGTAGTTGGAGAAGAAAATGAATATCCTGTTCAATTTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG231492 representing NM_001198760 Red=Cloning site Green=Tags(s) MRTGWATPRRPAGLLMLLFWFFDLAEPSGRAANDPFTIVHGNTGKCIKPVYGWIVADDCDETEDKLWKWV SQHRLFHLHSQKCLGLDITKSVNELRMFSCDSSAMLWWKCEHHSLYGAARYRLALKDGHGTAISNASDVW KKGGSEESLCDQPYHEIYTRDGNSYGRPCEFPFLIDGTWHHDCILDEDHSGPWCATTLNYEYDRKWGICL KPENGCEDNWEKNEQFGSCYQFNTQTALSWKEAYVSCQNQGADLLSINSAAELTYLKEKEGIAKIFWIGL NQLYSARGWEWSDHKPLNFLNWDPDRPSAPTIGGSSCARMDAESGLWQSFSCEAQLPYVCRKPLNNTVEL TDVWTYSDTRCDAGWLPNNGFCYLLVNESNSWDKAHAKCKAFSSDLISIHSLADVEVVVTKLHNEDIKEE VWIGLKNINIPTLFQWSDGTEVTLTYWDENEPNVPYNKTPNCVSYLGELGQWKVQSCEEKLKYVCKRKGE KLNDASSDKMCPPDEGWKRHGETCYKIYEDEVPFGTNCNLTITSRFEQEYLNDLMKKYDKSLRKYFWTGL RDVDSCGEYNWATVGGRRRAVTFSNWNFLEPASPGGCVAMSTGKSVGKWEVKDCRSFKALSICKKMSGPL GPEEASPKPDDPCPEGWQSFPASLSCYKVFHAERIVRKRNWEEAERFCQALGAHLSSFSHVDEIKEFLHF LTDQFSGQHWLWIGLNKRSPDLQGSWQWSDRTPVSTIIMPNEFQQDYDIRDCAAVKVFHRPWRRGWHFYD DREFIYLRPFACDTKLEWVCQIPKGRTPKTPDWYNPDRAGIHGPPLIIEGSEYWFVADLHLNYEEAVLYC ASNHSFLATITSFVGLKAIKNKIANISGDGQKWWIRISEWPIDDHFTYSRYPWHRFPVTFGEECLYMSAK TWLIDLGKPTDCSTKLPFICEKYNVSSLEKYSPDSAAKVQCSEQWIPFQNKCFLKIKPVSLTFSQASDTC HSYGGTLPSVLSQIEQDFITSLLPDMEATLWIGLRWTAYEKINKWTDNRELTYSNFHPLLVSGRLRIPEN FFEEESRYHCALILNLQKSPFTGTWNFTSCSERHFVSLCQKYSEVKSRQTLQNASETVKYLNNLYKIIPK TLTWHSAKRECLKSNMQLVSITDPYQQAFLSVQALLHNSSLWIGLFSQDDELNFGWSDGKRLHFSRWAET NGQLEDCVVLDTDGFWKTVDCNDNQPGAICYYSGNETEKEVKPVDSVKCPSPVLNTPWIPFQNCCYNFII TKNRHMATTQDEVHTKCQKLNPKSHILSIRDEKENNFVLEQLLYFNYMASWVMLGITYRNKSLMWFDKTP LSYTHWRAGRPTIKNEKFLAGLSTDGFWDIQTFKVIEEAVYFHQHSILACKIEMVDYKEEYNTTLPQFMP YEDGIYSVIQKKVTWYEALNMCSQSGGHLASVHNQNGQLFLEDIVKRDGFPLWVGLSSHDGSESSFEWSD GSTFDYIPWKGQTSPGNCVLLDPKGTWKHEKCNSVKDGAICYKPTKSKKLSRLTYSSRCPAAKENGSRWI QYKGHCYKSDQALHSFSEAKKLCSKHDHSATIVSIKDEDENKFVSRLMRENNNITMRVWLGLSQHSVDCP SSTWIQFQDSCYIFLQEAIKVESIEDVRNQCTDHGADMISIHNEEENAFILDTLKKQWKGPDDILLGMFY DTDDASFKWFDNSNMTFDKWTDQDDDEDLVDTCAFLHIKTGEWKKGNCEVSSVEGTLCKTAIPYKRKYLS DNHILISALVIASTVILTVLGAIIWFLYKKHSDSRFTTVFSTAPQSPYNEDCVLVVGEENEYPVQFD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198760
ORF Size	5451 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198760.1, NP_001185689.1
RefSeq Size	8751 bp
RefSeq ORF	5454 bp
Locus ID	100526664
UniProt ID	O60449
Gene Summary	This locus represents naturally occurring read-through transcription between the neighboring lymphocyte antigen 75 (LY75) and CD302 molecule (CD302) genes. Alternative splicing results in multiple transcript variants encoding fusion products that share sequence identity with each individual gene product. [provided by RefSeq, Nov 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.