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IRX5 (NM_001252197) Human Tagged ORF Clone
CAT＃: RG234228
- TrueORF®
IRX5 (tGFP-tagged) - Homo sapiens iroquois homeobox 5 (IRX5), transcript variant 2
ORF Plasmid: DDK
"NM_001252197" in other vectors (2)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HMMS; IRX-2a; IRXB2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234228 representing NM_001252197
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTATCCGCAGGGCTACTTGTACCAGCCGTCCGCCTCGCTGGCGCTCTACTCGTGCCCGGCGTACA GCACCAGCGTCATTTCGGGGCCCCGCACGGATGAGCTCGGCCGCTCTTCTTCGGGCTCCGCGTTCTCGCC CTACGCTGGCTCGACTGCCTTCACGGCGCCCTCGCCGGGCTACAACTCGCACCTCCAGTACGGCGCCGAC CCCGCGGCCGCCGCCGCCGCCGCCTTCTCCTCGTACGTGGGCTCTCCCTACGACCACACACCCGGCATGG CGGGCTCCTTGGGGTACCATCCTTACGCGGCGCCCCTGGGATCGTACCCTTACGGGGACCCAGCGTACCG GAAGAACGCCACAAGGGACGCCACGGCTACCCTCAAGGCCTGGCTCAACGAGCACCGCAAGAACCCCTAC CCCACCAAGGGCGAGAAGATCATGCTGGCCATCATCACCAAGATGACCCTCACCCAGGTGTCCACCTGGT TCGCCAACGCGCGCCGGCGCCTCAAGAAAGAGAATAAAATGACGTGGACGCCGCGGAACCGCAGCGAGGA CGAGGAAGAGGAGGAGAACATTGACCTGGAGAAGAACGACGAGGACGAGCCCCAGAAGCCCGAGGACAAG GGCGACCCCGAGGGCCCCGAAGCAGGAGCTGAGCAGAAGGCGGCTTCGGGCTGCGAACGGCTTCAGGGAC CACCCACCCCTGCAGGCAAGGAGACGGAGGGCAGCCTCAGCGACTCGGATTTTAAGGAGCCGCCCTCGGA GGGCCGCCTCGACGCGCTGCAGGGCCCCCCCCGCACCGGCGGGCCCTCCCCGGCTGGGCCAGCGGCGGCG CGGCTGGCGGAGGACCCGGCCCCTCACTACCCCGCCGGAGCGCCGGCGCCCGGCCCGCATCCAGCCGCGG GCGAGGTGCCTCCGGGTCCCGGCGGGCCCTCGGTTATCCATTCGCCGCCTCCGCCGCCGCCTCCTGCGGT GCTCGCCAAGCCCAAACTGTGGTCTTTGGCAGAGATCGCCACATCGTCGGACAAGGTCAAGGACGGGGGC GGCGGGAACGAGGGCTCTCCATGCCCACCGTGTCCCGGGCCCATAGCCGGGCAAGCCCTAGGAGGCAGCC GGGCGTCGCCGGCCCCGGCGCCGTCACGCTCGCCCTCGGCGCAGTGTCCTTTTCCAGGCGGGACGGTGCT GTCCCGGCCTCTCTACTACACCGCGCCCTTCTATCCCGGCTACACGAACTATGGCTCCTTCGGACACCTT CATGGCCACCCGGGGCCCGGGCCAGGCCCCACAACCGGTCCGGGGTCTCATTTCAATGGATTAAACCAGA CCGTGTTGAACCGAGCGGACGCTTTGGCTAAAGACCCGAAAATGTTGCGGAGCCAGTCTCAGCTAGACCT GTGCAAAGACTCTCCCTATGAATTGAAGAAAGGTATGTCCGACATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234228 representing NM_001252197
Red=Cloning site Green=Tags(s) MSYPQGYLYQPSASLALYSCPAYSTSVISGPRTDELGRSSSGSAFSPYAGSTAFTAPSPGYNSHLQYGAD PAAAAAAAFSSYVGSPYDHTPGMAGSLGYHPYAAPLGSYPYGDPAYRKNATRDATATLKAWLNEHRKNPY PTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWTPRNRSEDEEEEENIDLEKNDEDEPQKPEDK GDPEGPEAGAEQKAASGCERLQGPPTPAGKETEGSLSDSDFKEPPSEGRLDALQGPPRTGGPSPAGPAAA RLAEDPAPHYPAGAPAPGPHPAAGEVPPGPGGPSVIHSPPPPPPPAVLAKPKLWSLAEIATSSDKVKDGG GGNEGSPCPPCPGPIAGQALGGSRASPAPAPSRSPSAQCPFPGGTVLSRPLYYTAPFYPGYTNYGSFGHL HGHPGPGPGPTTGPGSHFNGLNQTVLNRADALAKDPKMLRSQSQLDLCKDSPYELKKGMSDI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001252197 |
| ORF Size | 1446 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001252197.1, NP_001239126.1 |
| RefSeq Size | 2075 bp |
| RefSeq ORF | 1449 bp |
| Locus ID | 10265 |
| UniProt ID | P78411 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of the iroquois homeobox gene family, which are involved in several embryonic developmental processes. Knockout mice lacking this gene show that it is required for retinal cone bipolar cell differentiation, and that it negatively regulates potassium channel gene expression in the heart to ensure coordinated cardiac repolarization. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Deciphering Transcriptional Networks during Human Cardiac Development
,null,
Cells
,PubMed ID 36497174
[IRX5]
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