Phlpp1 (NM_021657) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Phlpp; Plekhe1; Scop
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202102 representing NM_021657 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCCGCCGCAGCCCCGGCACAGCGACTCGCGGACCCCACGGGTGAGGACCGAGCCCCGGCGG CGGCGGCGGCGGCCGAGGGCGGACGGAGTCCGGATTCTGTGCTGAGCGCGGCGGCCCCGAGCGGCGGGAA CGGCGGCGCGGCGCGAGAGGAGGCCCCCTGCGAGGCGCCGCCGGGGCCGCTGCCCGGCCGCGCGGGAGGC ACCGGACGCAGGCGTCGGCGCGGGGTGCCCCAGCCCGCGGCCGGCGGGGCCGCCCCGGTGACCGCGGCCG GCGGCGGCGCCAACTCGCTGCTGCTGAGGAGAGGGCGGCTGAAGAGGAATCTGTCGGCCGCCGCTTCGTC GTCCTCATCGCCGTCCTCGGCGTCTTCGGCCGCTGGCGGCCTCCCGGCGTCCTGCTCGGCCTCGGCGTCG CTGTGCACCCGGAGTCTGGACAGGAAGACGCTGCTCCAGAAGCACCGGCAGCTGCTTCAGCTGCAGCCGT CGGACCGGGACTGGGTGAGGCACCAGCTCCAGCGGGGCTGTGTGCACGTCTTTGACCGCCACATGGCCTC GTCCTACCTGCGCCCAGTGCTCTGCACGCTGGACACCACAGCCGCCGAGGTGGCCGCCCGGCTTCTGCAG CTGGGCCACAAAGGCGGCGGGGTGGTGAAGGTGCTGGGCCACGGGCCACCTCCGGCCGCTGCCCCGGCCG CCAGCGATCAGACCCCGGCCACAGAGCTCGGAAGAGACGTGGAGCCACCGCCCTCGAGCAGCACGGTCGG TGCTGTCCGGGGCCCTGCGCGCGCGCCTCCTGCCGATCTGCCGCTGCCCGGGGGCGCCTGGACGCGCTGT GCACCTCGGGTCAACCCTGCGCCCTCGGACTCCAGCCCCGGAGAGCTGTTCGCGGGCGGCCCCTGTTCCC CGTCCCGCGCCCCGCGCCCGGCCTCCGACACCGAGAGCTTCAGCCTGAGCCCCAGCGCCGAGAGCGTGTC AGACCGGCTGGACCCCTATAGCAGCGGCGGCGGCTCCTCCTCTTCGTCCGAGGAACTAGAAGCTGACCCG GCCACGGTCTTGACGGGGCCTTCCGGGCCGCCCCACCACCCAGTCCGCTCCTCACAGCCTCGACCGCCTT CCCCGAAGACCTCGGCGTTACTGCAGCCGAAAGCCCCGACGGGCGTAGACGGCACTGGCCTAGTGGTGGG CGAAGGTCCCGGAGACGACAAGGCGGTGGCAGCTGCAGCTCCAGGCGTTCCTCTGTGGACTCCAGGGAGG ATCCGGGAGACCGTGCAAAAGACGTCTTCTCCGCCCTCCCTGTATGTACAGCTCCATGGGGAGACTACCC GGCGCCTGGAGGCGGACGAGAAGCCATTGCAGATCCAAAATGACTACCTCTTCCAACTGGGATTTGGGGA GCTGTGGAGGGTGCAGGAGGAAGGCATGGATTCGGAGATTGGCTGCCTCATCCGATTCTATGCAGGAAAG CCTCATAGCACTGGGAGCTCTGAGCGGATTCAGCTCTCGGGAATGTACAATGTCCGAAAAGGCAAAATGC AGTTGCCAGTGAACAGATGGACAAGACGCCAGGTCATTCTGTGTGGGACGTGCTTGATAGTGTCGTCTGT GAAAGACAGCTCGAGTGGGAAGATGCACGTTCTGCCTCTCATTGGTGGAAAAGTGGAAGAAGTGAAAAAG CACCAGCACTGTCTAGCGTTCAGCTCTTCCGGGCCCCAGAGCCAGACTTACTACATCTGCTTCGATACTT TCACGGAGTACTTACGATGGCTGCGTCAAGTCTCCAAGGTTGCATCACAACGCATAAGCTCAGTAGACCT CTCATGTTGTAGCCTTGAGCATCTGCCTGCCAACCTCTTTTACAGCCAAGACCTTACTCATCTCAATTTA AAACAGAACTTCCTAAGGCAGAACCCCAGCCTCCCAGCTGCCAGAGGACTTGGTGAACTGCAGAGATTCA CCAAATTGAAGAGCCTTAACCTTTCCAATAACCACCTAGGAGCCTTTCCGTCAGCAGTCTGCAGCATCCC AACCCTGGCAGAGCTGAATGTGTCTTGCAATGCCCTGCAAGAAGTCCCAGCAGCTGTTGGAGCTATGCAG AACTTACAGACGTTCTTGCTGGATGGAAATTTTCTCCAGTCCCTTCCTGCTGAGTTGGAGAACATGCACC AGCTCAGCTATTTGGGTCTTTCCTTTAATGAATTCACTGACATTCCAGAGGTATTGGAGAAGCTGACTGC TGTGGATAAACTGTGCATGGCTGGGAACTGTATGGAGACCCTTAGACTACAGGCCTTAAGAAGGATGCCT CATATTAAACACGTGGACCTAAGACTGAACATACTCAGAAAGCTTATAACAGATGAAGTGGACTTTCTGC AACATGTCACTCAGCTTGACCTGCGAGACAATAAACTTGGTGATCTAGATGCTATGATCTTCAACAACAT AGAAGTTCTGCACTGCGAGAGGAATCAGCTGGTGACACTGAACATTTGTGGCTATTTCCTAAAAGCACTC TATGCTTCTTCTAACGAACTCGTTCAACTTGATGTCTACCCAGTTCCAAATTATCTATCGTACATGGATG TCTCAAGAAACTGCCTAGAAAGTGTGCCTGAGTGGGTATGTGAAAGCCGGAAGTTAGAAGTTTTGGATAT TGGCCATAATCAAATATGTGAACTTCCTGCCCGCTTATTTTGTAATAGTAGTCTCCGGAAATTGCTGGCA GGACACAACCGGTTGGCAAGGCTTCCTGAAAGGCTGGAGAGAACATCAGTGGAGGTCTTGGACGTACAGC ATAACCAGATCATTGAGCTCCCACCAAACCTTCTCATGAAGGCTGACAGCCTGAGATTCCTCAATGCGTC CGCAAACAAACTGGAAACCCTGCCTCCAGCCACACTTTCTGAGGAGACAAGCAGTATATTACAGGAGTTG TACTTGACAAACAACAGCCTCACTGATAAGTGTGTGCCCTTGTTAACAGGGCACCCCCGCCTGAAGATCC TACACATGGCTTATAACCGGCTTCAGAGCTTCCCAGCAAGTAAAATGGCGAAACTGGAGGAGCTTGAAGA AATCGATATCAGTGGGAATAAGCTGAAAGCCATCCCAACAACCATCATGAACTGCAGGCGCATGCACACG GTGATTGCTCACTCGAACTGCATCGAAGTCTTTCCTGAAGTCATGCAGCTCCCAGAAGTCAAGTGTGTAG ATCTGAGCTGTAATGAGCTGAGTGAAATCACCCTACCAGAAAACCTGCCACCTAAACTGCAGGAACTAGA TCTGACCGGAAACCCACGCCTTGCCCTGGACCACAAAAGCTTGGAGCTGCTCAATAACATCCGCTGTTTC AAGATTGACCAGCCGTCAGCTGGAGATGCATCTGGAGCCCCAGCAGTATGGAGTCATGGTTACACTGAAG CATCGGGAGTAAAAAACAAGCTGTGTGTCGCAGCCCTGTCTGTGAATAACTTCCGTGACAACCGAGAGGC TCTCTACGGTGTGTTTGATGGAGACCGGAATGTGGAAGTGCCCTACCTTCTCCAGTGCACCATGAGTGAC ATCTTAGCTGAGGAGCTTCAGAAAACAAAAAATGAAGAAGAATACATGGTCAATACATTTATAGTCATGC AAAGGAAACTGGGAACGGCTGGGCAGAAACTCGGTGGTGCTGCTGTCCTGTGTCACATCAGGCATGACCC TGTGGACCTGGGAGGATCCTTTACCCTGACCTCTGCTAATGTTGGCAAGTGCCAAACGGTTCTCTGTCGA AACGGGAAGCCGCTGTCTCTGTCCAGGTCCTATACCATGAGCTGTGAAGAAGAGCGGAAGAGGATTAAGC AGCACAAAGCCATCATCACTGAGGATGGCAAGGTCAATGGAGTGACAGAGTCCACACGCATCCTGGGCTA CACCTTCCTTCACCCCAGTGTGGTGCCTCGCCCCCACGTACAGTCGGTGCTTCTGACTCCACAGGATGAG TTTTTCATCCTGGGCAGCAAAGGGCTGTGGGACAGCCTGTCCATCGAAGAGGCTGTGGAAGCCGTGCGCA ACGTTCCAGATGCTCTAGCCGCCGCCAAGAAGCTATGCACCCTGGCCCAGAGCTACGGCTGCCACGACAG CATCAGTGCTGTGGTGGTGCAACTCAGTGTCACGGAAGACAGCTTCTGCTGCTGTGAGCTCAGTGTGGGA GGAAGCATGCCACCGCCCAGTCCCGGAATCTTCCCGCCCTCCGTCAGCATGGTGATCAAGGACCGGCCCT CAGATGGGCTGGGCGTGCCGTCCTCCAGCAGCGGCATGGCATCTGAGATCAGCAGTGAATTGTCTACCTC TGAGATGAGTAGTGAGGTGGGCTCCACAGCTTCTGACGAGCCGCCGTCGGGAGCCCTGAGTGAGAGCAGC CCCGCCTACCCCAGCGAGCAGCGCTGCATGCTCCACCCGGTCTGCCTGTCCAACTCCTTCCAGCGCCAGC TGTCCAGCGCCACTTTCTCCAGTGCGTTCTCTGACAACGGCCTTGACAGTGATGACGAGGAACCCATTGA GGGCGTGTTCAGCAACGGCAGCCGGGTGGAGGTGGAAGTGGACATCCATTGCAGCCGGGCCAAGGAGAAG GAGAGACAGCAGCACCTGCTTCAGGTACCAGCTGAAGCCAGTGATGAGGGCATTGTCATCAGTGCCAATG AGGATGAGTCAGGTCTGTCCAAGAAGACAGACATTTCTGCTGTGGGGACCATTGGGCGACGCAGGGCTAA CGGCTCTGTACCTCCCCAGGAAAGGAGCCATAATGTAATAGAGGTTGCCACAGATGCACCTCTCCGGAAG CCGGGAGGCTATTTTGCAGCCCCTGCTCAACCAGATCCAGATGATCAGTTTATCATCCCCCCAGAGCTGG AAGAGGAAGTCAAAGAAATCATGAAACATCACCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA GCAGCAGCAGCAGCCACCACCTCCACCCCAGCCGCCACAGGCACAGGCACAGGCACAGGCACAGGCACAG AGGCCCTTCCAGATGGATCACCTGCCGGACTGTTACGACACACCACTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_021657
Insert Size	5091 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021657.1, NP_067689.1
RefSeq Size	6251 bp
RefSeq ORF	5091 bp
Locus ID	59265
UniProt ID	Q9WTR8
Gene Summary	a circadian rhythm protein; may have a role in the intracellular signaling in the suprachiasmatic nucleus [RGD, Feb 2006]

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