Mapk10 (NM_012806) Rat Untagged Clone

CAT#: RN203224

Mapk10 (untagged ORF) - Rat mitogen activated protein kinase 10 (Mapk10), (10 ug)



  "NM_012806" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Jnk3; SAPb; SAPKC; Serk2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN203224 representing NM_012806
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAAAAGCAAGGTAGATAACCAGTTCTACAGTGTGGAAGTGGGAGACTCAACCTTCACAGTTCTAA
AGCGCTACCAGAACCTGAAGCCGATCGGCTCTGGGGCTCAGGGAATAGTTTGTGCTGCGTATGACGCTGT
CCTCGACAGAAATGTGGCCATTAAGAAGCTCAGCAGACCCTTCCAGAACCAAACTCATGCCAAGAGGGCT
TACCGGGAGCTGGTCCTCATGAAGTGTGTGAACCATAAAAACATTATTAGCTTATTAAATGTCTTTACAC
CCCAGAAAACACTGGAGGAGTTCCAAGATGTTTACTTAGTGATGGAACTGATGGACGCCAACTTGTGTCA
GGTGATTCAGATGGAGCTGGACCACGAGCGGATGTCGTACTTGCTGTACCAGATGCTGTGCGGCATCAAA
CACCTCCACTCCGCTGGGATCATCCACAGGGACTTAAAACCCAGTAACATCGTAGTCAAGTCTGATTGCA
CACTGAAAATCCTGGACTTTGGACTGGCCAGGACAGCGGGCACAAGCTTCATGATGACTCCGTATGTGGT
GACGAGATATTACAGAGCCCCCGAGGTCATCCTGGGCATGGGCTACAAGGAGAACGTGGACATATGGTCT
GTGGGCTGCATCATGGGAGAAATGGTTCGTCACAAAATCCTCTTTCCCGGAAGGGACTATATTGACCAGT
GGAACAAAGTCATAGAGCAGCTAGGAACTCCGTGTCCAGAATTCATGAAGAAATTGCAGCCCACCGTCAG
AAACTACGTGGAGAACCGGCCCAAGTATGCAGGCCTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTC
CCAGCGGATTCCGAGCACAATAAACTTAAAGCCAGCCAAGCCAGGGACTTGTTGTCAAAGATGTTAGTGA
TTGACCCAGCGAAGAGGATATCGGTGGATGACGCATTGCAGCATCCGTACATCAACGTTTGGTACGACCC
TGCTGAAGTGGAGGCGCCTCCGCCTCAGATATATGACAAGCAACTGGATGAAAGGGAGCACACCATCGAA
GAATGGAAAGAACTCATCTACAAGGAAGTAATGAACTCAGAAGAGAAGACTAAGAACGGCGTAGTCAAAG
GCCAGCCCTCACCTTCAGGTGCAGCAGTGAACAGCAGTGAGAGTCTCCCTCCATCCTCGTCTGTCAACGA
CATCTCCTCCATGTCCACCGACCAGACCCTCGCATCCGACACTGACAGCAGCCTGGAAGCCTCGGCGGGA
CCGCTGGGTTGTTGCAGGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_012806
Insert Size 1281 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012806.2, NP_036938.2
RefSeq Size 2786 bp
RefSeq ORF 1281 bp
Locus ID 25272
UniProt ID P49187
Gene Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]
Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 5' region, which results in translation initiation from an in-frame, downstream start codon compared to variant 1. The encoded isoform (2, also known as SAPK beta) has a shorter N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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