Bag1 (NM_001106647) Rat Untagged Clone
CAT＃: RN203877
Bag1 (untagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Bag-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN203877 representing NM_001106647
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGCGGATCGCGGCGGCGCGCGGCGACCGCGAGGCGACCAGGAGCCGCTGGGACCCCGGCTGCGCGCCC CTCGGTCGGCCCGGGAGACGCGCCAGTCGGAGTCCCGGGCCGAGCGTGGCTTGCCTCCCTCTCAGCGTTC ATCTGTCCGCAGTGCAGCCAGCGGGCATGACCGATCCACCAGGGGCGCCGCCTCCGGCGCTTGCAAGCCG CGGGTGAAGAAGAAAGTCCGGCCCCGCTCTTCTCAGAGCGAGAAGGTAGCTCACAGCAAGGAGTTGACTA GAAGTAAGAAGTTGACCCGCAGCAAGAAAGTGACCGGGACCCAGGAGGCGACCCAAGTAGAGGAGGTGAC CACGATCGAGGAGGCGACCCAGACCGAGGAGATTACGGTAGCGGAAGAGGTGACCCAGACCGAGAACATG GCCCAGACCGAGGAGATGGTCCAGACGGAGGAAATGGAACCACCCACACTCAGCGTGGTCGTCACCCACA GCAATGAGAGGTATGACCTTCTTGTTACCCCACAGCAAGGTAACAGTGAGCCAATTGTCCAAGACCTGGC TCAGCTTGTTGAAGAGGCCACAGGAGTTCCACTACCTTTTCAGAAGCTCATATTTAAGGGCAAATCTCTG AAAGAAATGGAAACACCCTTGTCAGCACTTGGAATGCAAAATGGTTGCCGAGTCATGTTAATTGGTGAAA AGAGCAATCCAGAAGAAGAGGCTGAGTTGAAAAAGCTGAAGGACTTGGAGGTATCTGTGGAGAAGACAGC TAACCACCTGGAAGAGTTGAATAAAGAGCTTTCTGACATCCAGCAGGGTTTTCTGGCTAAGGAATTACAA GCGGAGGCTCTCTGCAGACTTGATAGGAAAATAAAGGCCACAATTGAGCAATTCATGAAGATCTTGGAGG AGATTGACACAATGGTCCTACCAGAAAACTTTAAAGACAGCAGGCTAAAAAGGAAGAATTTGGTGAAAAA GGTTCAGGTGTTCCTAGCAGAGTGTGATACAGTGGAGCAGTACATCTGCCAAGAGACAGAGCGGCTGCAG TCTACAAACTTGGCCCTGCCTGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001106647 |
| Insert Size | 1077 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001106647.3, NP_001100117.2 |
| RefSeq Size | 1308 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 297994 |
| UniProt ID | B0K019 |
| Gene Summary | The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. Studies in human and mouse suggest that the protein encoded by this gene (referred to as Bcl2-associated athanogene) binds to Bcl2 protein. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative, downstream, AUG translation initiation site. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longer isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (BAG-1S or p32) is derived from a downstream AUG start codon. The longer isoform (1L) is represented in this RefSeq. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR203877 | Bag1 (Myc-DDK-tagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug) |
CNY 3656.00 |
|
| RR203877L3 | Lenti ORF clone of Bag1 (Myc-DDK-tagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug) |
CNY 6080.00 |
|
| RR203877L4 | Lenti ORF clone of Bag1 (mGFP-tagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug) |
CNY 6650.00 |
