Jag1 (NM_019147) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203938 representing NM_019147 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTCCCCACGGACGCGCGGCCGGCCGGGGCGCCCCTTGAGCCTTCTGCTCGCCCTGCTCTGTGCCC TGCGAGCCAAGGTGTGCGGGGCCTCGGGTCAATTTGAGCTGGAGATCCTGTCCATGCAGAACGTGAACGG AGAGTTACAGAATGGGAACTGTTGCGGCGGAGCCCGGAACCCTGGCGACCGCAAGTGCACCCGCGACGAG TGTGACACGTACTTCAAAGTGTGCCTCAAGGAGTATCAGTCCCGCGTCACGGCCGGGGGACCCTGCAGCT TCGGCTCAGGGTCTACGCCTGTCATCGGGGGCAATACCTTCAATCTCAAGGCCAGCCGCGGCAACGACCG TAATCGCATCGTACTGCCTTTCAGTTTCGCCTGGCCGAGGTCCTACACTTTGCTGGTGGAGGCATGGGAT TCCAGTAATGACACTATTCAACCTGATAGCATAATTGAAAAGGCTTCTCACTCAGGCATGATAAACCCCA GCCGGCAGTGGCAGACACTGAAACAGAACACAGGGATTGCCCACTTTGAGTATCAGATCCGAGTGACCTG TGATGACCACTACTATGGCTTTGGCTGCAACAAGTTCTGTCGGCCCAGAGATGACTTCTTTGGACATTAT GCCTGCGACCAGAATGGCAACAAAACCTGCATGGAAGGCTGGATGGGTCCTGAGTGTAACAAAGCAATAT GCCGACAGGGCTGCAGTCCCAAGCATGGCTCCTGCAAACTCCCAGGTGACTGCAGGTGCCAGTATGGTTG GCAGGGCCTGTACTGTGATAAGTGCATCCCGCACCCAGGATGTGTCCACGGCACCTGCAATGAACCCTGG CAGTGCCTCTGTGAAACCAACTGGGGTGGACAGCTCTGTGACAAAGATCTGAATTACTGTGGGACTCATC AGCCCTGTCTCAACCGGGGAACATGTAGCAACACTGGCCCTGACAAATACCAGTGTTCCTGCCCCGAGGG CTACTCAGGACCGAACTGTGAAATTGCTGAGCATGCCTGCCTCTCTGATCCCTGCCACAACAGAGGCAGC TGCAAGGAGACCTCCTCGGGCTTTGAGTGTGAGTGTTCTCCAGGCTGGACTGGACCCACGTGTTCCACAA ACATCGATGACTGTTCTCCAAATAACTGTTCCCATGGAGGCACCTGCCAGGATCTAGTGAATGGATTCAA GTGCGTGTGCCCACCCCAGTGGACTGGCAAGACTTGTCAGTTAGATGCAAATGAGTGCGAGGCCAAGCCT TGTGTAAATGCCAGATCCTGTAAGAATCTGATTGCCAGCTACTACTGTGATTGTCTTCCTGGCTGGATGG GTCAGAATTGTGACATAAATATCAATGACTGCCTTGGCCAGTGTCAGAATGATGCCTCCTGTCGGGATTT GGTTAATGGTTATCGCTGTATCTGTCCACCTGGCTATGCGGGCGATCACTGTGAAAGAGACATCGATGAA TGTGCTAGCAACCCCTGCTTGAATGGGGGTCACTGTCAGAATGAAATTAACAGATTCCAGTGTCTGTGTC CCACTGGTTTCTCTGGAAACCTCTGTCAGCTGGACATCGATTACTGCGAGCCCAACCCTTGCCAGAATGG CGCCCAATGCTACAATCGTGCCAGTGACTATTTCTGCAAGTGCCCTGAGGACTACGAGGGCAAGAACTGC TCACACCTGAAAGACCACTGCCGCACCACTCCCTGTGAAGTGATTGACAGCTGCACCGTGGCCATGGCCT CCAACGATACTCCTGAAGGGGTGCGGTATATCTCTTCCAACGTCTGTGGTCCCCATGGGAAGTGCAAGAG CGAGTCAGGAGGCAAATTCACCTGTGACTGTAACAAAGGCTTCACCGGCACCTACTGCCATGAAAATATC AACGACTGTGAGGGCAACCCCTGTACGAACGGTGGCACGTGCATCGACGGCGTTAACTCCTACAAGTGTA TCTGTAGTGACGGCTGGGAGGGCGCGCACTGTGAGAACAACATAAATGACTGTAGCCAGAACCCTTGCCA CTATGGGGGTACATGTCGAGACCTGGTCAATGACTTTTACTGTGACTGCAAAAATGGGTGGAAAGGGAAA ACCTGCCACTCGCGTGACAGCCAGTGTGACGAAGCCACGTGTAATAACGGTGGTACCTGCTATGATGAAG TGGATACGTTTAAGTGCATGTGTCCTGGTGGCTGGGAAGGAACAACCTGTAATATAGCTAGAAACAGTAG CTGCCTGCCGAACCCCTGTCATAATGGAGGCACCTGCGTGGTCAATGGAGACTCCTTCACCTGTGTCTGC AAAGAAGGCTGGGAGGGGCCGATTTGTACTCAAAATACCAACGACTGCAGTCCTCACCCTTGTTACAACA GCGGAACCTGTGTGGATGGAGATAACTGGTACCGGTGCGAATGTGCCCCTGGTTTTGCTGGGCCAGACTG CAGGATAAACATCAATGAGTGTCAGTCTTCCCCTTGTGCCTTTGGGGCCACCTGTGTGGATGAGATCAAT GGCTACCAGTGTATCTGCCCTCCAGGACATAGTGGTGCCAAGTGCCATGAAGTTTCAGGGAGATCTTGCA TCACCATGGGGAGAGTGATACTTGATGGGGCCAAGTGGGATGACGACTGTAACACCTGCCAGTGCCTGAA TGGACGGGTGGCCTGCTCCAAGGTCTGGTGTGGCCCGAGACCTTGCCGGCTCCACAAAGGGCACGGCGAG TGTCCCAATGGGCAGAGCTGCATCCCAGTCCTGGATGACCAGTGTTTCGTGCGCCCCTGCACTGGTGCTG GCGAGTGTCGGTCCTCCAGCCTCCAGCCAGTGAAGACCAAGTGTACATCGGACTCTTATTACCAGGACAA TTGTGCGAACATCACTTTCACCTTTAACAAGGAGATGATGTCTCCAGGTCTCACCACTGAGCACATTTGC AGCGAATTGAGGAATCTGAATATTCTGAAGAATGTTTCTGCTGAGTATTCAATCTACATAGCCTGTGAGC CTTCCTTATCAGCAAACAATGAAATACACGTGGCCATTTCTGCCGAAGACATCCGGGATGATGGAAACCC TGTCAAGGAAATTACTGATAAAATAATAGATCTCGTTAGTAAACGGGATGGGAACAGCTCACTTATCGCT GCTGTTGCAGAAGTCAGAGTTCAGAGGCGCCCTCTGAAAAACAGAACAGATTTCCTGGTTCCTCTGCTGA GCTCTGTCTTAACAGTGGCTTGGGTCTGTTGCTTGGTGACAGCCTTCTACTGGTGTGTGCGGAAGCGGCG GCGGAAGCCCAGCAGCCACACCCACTCTGCCCCTGAGGACAACACCACCAACAATGTGCGCGAACAGCTG AACCAAATCAAAAACCCCATCGAGAAACACGGAGCCAACACAGTCCCCATCAAGGATTATGAGAACAAAA ACTCCAAAATGTCAAAAATCAGGACACACAACTCGGAAGTGGAAGAGGATGATATGGATAAGCACCAGCA GAAAGTCCGGTTTGCCAAACAGCCCGTGTACACGCTGGTAGACAGAGAGGAGAAGGTCCCCCAGCGCACG CCGACAAAACACCCGAACTGGACAAACAAACAGGACAACAGAGACTTGGAAAGTGCCCAGAGCTTGAACC GGATGGAGTACATCGTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019147
Insert Size	3660 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019147.1, NP_062020.1
RefSeq Size	5575 bp
RefSeq ORF	3660 bp
Locus ID	29146
UniProt ID	Q63722
Gene Summary	ligand responsible for activating Notch1 [RGD, Feb 2006]

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