Notch3 (NM_020087) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204371 representing NM_020087 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCGGGGGCCCGGGGCCGCCGCCGCCGTCGTCGCCTGATGGCCCTGCCACCGCCACCACCGCCCA TGCGGGCGCTGCCCCTGCTGCTGCTGCTGCTAGCGGGGCTGGGGGCTGCAGCACCCCCTTGTCTGGATGG AAGCCCATGTGCAAATGGAGGTCGTTGCACCCACCAGCAGCCCTCCCGGGAGGCTGCTTGCCTGTGCCTG CCAGGCTGGGTGGGTGAGCGGTGCCAGCTGGAAGACCCTTGCCACTCTGGCCCCTGTGCCGGCCGAGGCG TTTGCCAGAGTTCAGTGGTGGCGGGCGTCGCCCGGTTCTCCTGTCGTTGTCTCCGTGGCTTCCGAGGCCC AGACTGCTCCCTGCCAGACCCCTGCTTCAGCAGCCCCTGTGCTCACGGTGCCCCCTGCTCAGTTGGGTCG GATGGCCGATATGCCTGTGCCTGCCCACCTGGCTACCAGGGTCGCAACTGCCGAAGCGACATAGATGAGT GCCGAGCTGGTGCGTCTTGCCGTCACGGTGGTACCTGCATCAATACACCCGGATCCTTCCACTGCCTGTG TCCACTTGGCTATACAGGGCTGCTGTGTGAAAACCCCATAGTGCCCTGCGCCCCTTCCCCATGCCGTAAT GGTGGCACCTGTAGGCAGAGTAGTGATGTCACATATGACTGTGCTTGTCTTCCTGGCTTCGAGGGCCAGA ACTGTGAAGTCAATGTGGATGACTGTCCTGGACATCGGTGTCTCAATGGGGGAACGTGTGTGGATGGTGT CAATACTTATAACTGCCAGTGCCCTCCTGAGTGGACAGGCCAGTTCTGCACAGAAGATGTGGATGAGTGC CAGTTGCAGCCCAACGCTTGCCACAATGGGGGCACCTGCTTCAACCTACTGGGCGGCCACAGCTGCGTAT GCGTCAACGGCTGGACGGGCGAGAGCTGCAGTCAGAATATTGATGACTGTGCTACAGCCGTTTGCTTCCA TGGGGCCACTTGCCATGACCGTGTGGCCTCTTTCTATTGTGCCTGCCCTATGGGGAAGACCGGCCTCTTG TGTCATCTGGATGATGCATGTGTCAGCAACCCCTGCCATGAAGATGCTATCTGTGACACAAACCCTGTGA GTGGTCGGGCCATCTGCACCTGTCCACCCGGCTTCACCGGAGGGGCGTGTGACCAGGATGTGGATGAGTG CTCTATTGGTGCTAACCCCTGTGAACATTTGGGTCGGTGTGTGAACACACAGGGCTCATTCTTGTGCCAG TGTGGCCGTGGCTATACTGGACCTCGCTGTGAGACTGATGTCAATGAGTGTCTGTCTGGGCCCTGCCGCA ACCAGGCCACGTGTCTTGACCGAATTGGCCAGTTCACTTGCATCTGCATGGCAGGCTTCACAGGAACCTT CTGTGAGGTGGACATTGATGAATGTCAGAGCAGCCCATGTGTCAATGGTGGTGTCTGCAAGGATAGAGTC AATGGCTTCAGCTGCACCTGCCCATCAGGATTCAGTGGGTCCACGTGTCAGCTGGATGTGGATGAATGTG CAAGCACTCCTTGCCGGAACGGTGCCAAGTGTGTGGACCAGCCTGACGGCTATGAGTGTCGCTGTGCAGA GGGCTTTGAGGGCACCCTGTGTGAGCGAAACGTGGACGACTGCTCTCCGGATCCCTGTCACCACGGGCGC TGTGTCGACGGCATTGCTAGCTTCTCATGCGCTTGTGCGCCAGGCTACACGGGCATACGCTGCGAGAGCC AGGTGGATGAGTGCCGCAGCCAGCCCTGCCGATACGGGGGCAAATGTCTAGACTTGGTGGACAAGTACCT CTGCCGCTGTCCTCCCGGAACCACAGGTGTGAACTGCGAAGTCAACATCGATGATTGCGCCAGCAACCCC TGTACCTTTGGAGTTTGCCGTGATGGCATCAACCGCTATGATTGTGTCTGTCAGCCTGGATTCACAGGGC CCCTCTGCAACGTGGAGATCAATGAGTGTGCATCCAGCCCATGTGGAGAGGGTGGCTCCTGTGTGGACGG GGAAAATGGCTTCCACTGCCTCTGCCCACCTGGCTCCCTGCCTCCACTCTGCCTACCTGCGAACCACCCC TGTGCCCACAAGCCCTGTAGTCATGGAGTCTGCCACGATGCACCAGGCGGGTTTCAATGTGTGTGCGATC CCGGTTGGAGTGGCCCTCGTTGCAGCCAGAGCCTGGCTCCAGATGCCTGTGAGTCCCAGCCCTGCCAGGC TGGTGGCACCTGCACCAGTGATGGAATAGGCTTCCACTGTACCTGTGCCCCTGGATTCCAGGGTCATCAA TGTGAGGTGCTGTCCCCCTGCACTCCAAGTCTCTGTGAGCACGGAGGCCACTGTGAGTCTGACCCTGACC AGCTGACTGTCTGTTCCTGTCCTCCAGGCTGGCAAGGCCCTCGATGCCAGCAGGATGTGGATGAATGTGC TGGTGCCTCACCTTGTGGCCCCCATGGTACCTGCACCAACCTGCCAGGGAGCTTCAGGTGCATCTGCCAT GGAGGATACACTGGCCCTTTCTGTGATCAGGACATTGACGACTGTGACCCCAACCCGTGCCTCAATGGTG GCTCCTGCCAGGATGGTGTGGGCTCCTTTTCCTGTTCTTGCCTCAGTGGCTTTGCTGGTCCTCGCTGTGC TCGGGATGTGGATGAATGTCTGAGCAGTCCCTGTGGCCCTGGCACCTGCACTGATCATGTAGCCTCTTTC ACCTGTACCTGTCCACCTGGCTATGGAGGCTTCCACTGTGAGACTGACTTGCTGGACTGCAGCCCCAGTT CCTGTTTCAATGGAGGGACCTGTGTGGATGGCGTGAACTCCTTCAGCTGTCTGTGTCGTCCTGGCTACAC AGGCACACATTGCCAATATAAGGTTGACCCCTGCTTCTCCCGGCCCTGTCTACATGGGGGCATCTGCAAC CCCACCCACTCAGGATTTGAATGCACCTGCCGGGAGGGCTTCACTGGGAATCAGTGTCAGAACCCAGTGG ACTGGTGCAGCCAGGCACCCTGTCAGAATGGGGGTCGCTGTGTCCAGACTGGGGCTTACTGCATCTGTCC GCCTGAATGGAGTGGCCCCCTGTGCGACATACCAAGCCTACCCTGCACGGAGGCTGCAGCCCACATGGGG GTGCGGTTGGAGCAGCTGTGTCAGGCGGGTGGCCAGTGCATAGACAAGGACCACTCCCACTACTGTGTGT GTCCGGAGGGCCGTATGGGTAGTCACTGCGAACAGGAGGTGGATCCCTGCACAGCCCAGCCTTGCCAGCA TGGGGGCACCTGCCGTGGTTACATGGGCGGCTATGTGTGTGAGTGTCCAACCGGCTACTCTGGTGACAGT TGTGAGGATGATGTGGATGAGTGCGCTTCCCAGCCCTGCCAGAATGGAGGCTCCTGTATTGATCTTGTGG CTCACTATCTCTGCTCCTGTCCCCCTGGCACACTGGGAGTTCTCTGTGAGATCAATGAGGATGACTGTGG CCCAGGCCCATCCTTGGACTCAGGCCTCCGGTGCCTACACAACGGCACCTGTGTGGACCTGGTGGGTGGC TTCCGCTGTAACTGTCCCCCAGGGTACACAGGTCTGCACTGTGAGGCGGACATCAATGAGTGCCGACCCG GCACCTGTCATGCAGCACATACCCGGGACTGCCTACAAGATCCAGGTGGGCACTTCCGCTGCATCTGTCT CCCTGGCTTCACAGGGCCTCGATGTCAGACTGCTCTGTTCCCCTGTGAGTCCCAACCGTGTCAGCATGGA GGCCAGTGCCGTCCCAGCCTAGGCCGTGGAGGTGGGCTGACCTTCACCTGTCACTGTGTCCAGCCATTCT GGGGTCTGCGTTGCGAGCGGGTGGCACGCTCCTGCCGGGAGCTGCAGTGCCCAGTGGGTATCCCATGCCA GCAGACAGCCCGTGGGCCGCGCTGTGCTTGTCCTCCCGGGCTGTCTGGGCCCTCCTGCCGGGTTTCTAGG GCGTCACCCTCTGGAGCTACTAACACCAGCTGCGCTGCTACTCCTTGTCTGCATGGGGGCTCCTGCCTAC CTGTACAGAGTGTCCCTTTCTTCCGCTGTGTATGCGCTCCGGGCTGGGGTGGCCCGCGTTGCGAGACCCC TTCCGCAGCCCCTGAGGTCCCTGAGGAGCCACGGTGCCCGCGAGCCGCCTGTCAGGCCAAGCGAGGGGAC CAGAACTGCGACCGTGAGTGCAACAGCCCAGGCTGTGGCTGGGACGGCGGTGACTGCTCGCTGAATGTGG ACGACCCCTGGAGGCAGTGTGAGGCACTGCAGTGCTGGCGTCTCTTCAACAACAGCCGGTGCGATCCAGC CTGCAGCTCTCCTGCCTGCCTCTATGACAACTTTGACTGCTACTCTGGTGGCCGCGACCGCACCTGCAAC CCGGTTTATAAGAAGTACTGCGCCGACCACTTTGCAGATGGCCGTTGTGACCAGGGCTGTAACACCGAGG AGTGTGGCTGGGATGGGCTGGACTGTGCCAGTGAGGTCCCGGCCCTTTTGGCCCGGGGGGTTCTGGTCCT CACAGTTCTTCTGCCTCCTGAAGAGTTGCTTCGCTCTAGTGCTGACTTCCTGCAGCGACTCAGCGCTATT CTGCGCACCTCGCTGCGCTTCCGCCTGGATGCACGTGGCCAAGCCATGGTCTTTCCCTACCACCGGCCAA GTCCTGGCTCCGAATCCCGGGTTCGTCGTGAGCTTGGCCCAGAGGTGATCGGCTCTGTAGTGATGCTGGA GATTGACAACCGGCTCTGTCTGAAGTCAGCGGAGAACGACCACTGCTTCCCTGACGCCCAGAGTGCTGCC GACTACCTGGGAGCCTTGTCGGCAGTGGAGCGGCTTGATTTCCCATACCCACTACGGGATGTGCGAGGAG AGCCCTTGGAGCCTCCAGAGCAGAGCGTGCCACTGCTGCCGCTGCTGGTGGCAGGGGCTGTCTTTCTGCT GGTCATCTTCGTCCTGGGTGTCATGGTTGCCAGGCGCAAGCGAGAGCACAGCACCCTCTGGTTTCCTGAG GGTTTTGCATTACACAAGGACATAGCTGCTGGCCACAAGGGCCGGAGGGAGCCCGTGGGACAAGACGCAC TGGGCATGAAGAACATGACCAAGGGTGAGAGTCTGATGGGGGAGGTGGCCACAGACTGGAATGACTCAGA ATGCCCAGAGGCCAAGAGACTGAAGGTAGAGGAGCCAGGCATGGGGGCCGAGGAGCCCGTGGACTGTCGC CAGTGGACCCAACACCACCTGGTTGCTGCCGATATCCGTGTGGCACCAGCCATGGCACTGACTCCACCTC AGGGAGATGCAGATGCCGATGGCATGGATGTCAACGTACGAGGGCCTGATGGCTTCACCCCACTTATGCT GGCCTCGTTCTGTGGGGGAGCCCTGGAGCCGATGCCAGCTGAGGAGGATGAGGCCGATGACACGTCAGCC AGCATTATATCAGATCTGATCTGCCAAGGAGCCCAGCTCGGGGCACGAACTGACCGAACTGGCGAGACCG CCCTGCATTTGGCCGCCCGATATGCTCGAGCCGATGCGGCCAAGCGGCTACTGGATGCTGGGGCAGACAC CAACGCCCAGGATCATTCCGGCCGCACCCCTCTGCACACCGCAGTGACAGCTGATGCCCAGGGTGTCTTC CAGATTCTCATCAGGAACCGCTCCACTGACCTGGATGCCCGAATGGCAGATGGCTCTACTGCATTGATCC TGGCAGCCCGCCTGGCGGTGGAGGGAATGGTGGAAGAGCTCATCGCCAGCCACGCAGATGTCAATGCAGT GGATGAGCTCGGGAAATCGGCCTTACACTGGGCTGCAGCCGTGAACAATGTGGAGGCTACCTTGGCTCTG CTGAAAAATGGAGCCAACAAGGACATGCAGGACAGCAAGGAGGAGACTCCACTGTTCTTGGCCGCCCGTG AGGGCAGCTATGAGGCTGCCAAGCTGCTGCTGGATCATTTTGCCAACCGGGAGATCACAGATCACTTGGA CAGGCTGCCCCGGGATGTGGCCCAGGAGCGGCTGCACCAGGACATTGTGCGGTTGCTGGACCAGCCCAGT GGCCCCCGAAGTCCCTCTGGTCCCCATGGCTTGGGGCCATTGCTCTGCCCACCAGGAGCCTTCCTTCCTG GCCTCAAAGCGGTGCAGTCTGGGACCAAGAAGAGCAGGAGGCCACCTGGCAAGACTGGGCTGGGGCCCCA GGGAACTCGGGGTCGGGGCAAGAAGCTGACACTGGCCTGTCCAGGCCCTCTGGCAGACAGCTCTGTCACA CTGTCACCAGTAGACTCGCTGGACTCACCACGGCCTTTCGGTGGGCCCCCTGCTTCCCCTGGAGGCTTCC CCTTGGAGGGTCCCTATGCCACCACAGCCACCACGGTGTCCTTGGCACAGCTGGGTGCAAGTCGGGCGGG TCCTCTGGGGCGCCAGCCTCCTGGGGGCTGTGTGCTCAGCCTTGGTTTGCTCAACCCTGTGGCTGTTCCC CTTGACTGGGCCCGGCTGCCTCCACCTGCCCCTCCAGGGCCCTCATTCCTGCTGCCCCTGGCTCCGGGAT CCCAGCTGCTCAACCCGGCAACCCCAGTTTCTCCCCATGAGCGGCCCCCACCCTACCTGGCTGCTCCAGG ACATGGAGAGGAATACCCTGCAGCAGGGACCCACAGCAGCCCCACAAAGGCCCGCTTCCTGCGGGTTCCC AGCGAGCATCCTTATTTGACCCCATCTCCTGAGTCCCCAGAGCACTGGGCCAGCCCGTCCCCTCCCTCCC TCTCAGACTGGTCCGACTCAACGCCTAGTCCAGCAACTGCTACCAGTGCCACAGCCGCTGGAGCCCTGCC TGCTCAACCACACCCCATATCTGTTCCCTCCCTCCCTCAGTCCCAGACTCAGCTGGGGCCCCAGCCAGAA GTAACCCCCAAGAGGCAGGTGATGGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_020087
Insert Size	6960 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020087.2, NP_064472.2
RefSeq Size	7319 bp
RefSeq ORF	6960 bp
Locus ID	56761
UniProt ID	Q9R172
Gene Summary	Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Acts instructively to control the cell fate determination of CNS multipotent progenitor cells, resulting in astroglial induction and neuron/oligodendrocyte suppression.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.