Bap1 (NM_001107292) Rat Untagged Clone
CAT＃: RN205856
Bap1 (untagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug)
CNY 9600.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN205856 representing NM_001107292
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAAGGGCTGGCTGGAGCTGGAGAGTGACCCGGGCCTCTTCACCCTCCTGGTGGAAGATTTCGGTA AGAACCCCTTTACCCCCGATCGTCGGGCCCTGGCTGGTGTCAAAGGGGTGCAAGTGGAGGAGATCTATGA CCTTCAGAGTAAATGCCAGGGGCCTGTATATGGATTTATCTTCCTGTTCAAATGGATCGAAGAGCGCAGG TCCCGCCGCAAGGTTTCTACGTTGGTGGATGATACATCTGTGATTGATGATGACATCGTGAATAGCATGT TCTTTGCTCACCAGCTGATTCCCAACTCTTGTGCCACTCACGCCTTGCTGAGCGTGCTTCTGAACTGCAG CAATGTGGATTTGGGGCCCACGCTGAGTCGAATGAAGGATTTCACCAAAGGCTTCAGCCCTGAGAGCAAA GGATATGCGATTGGCAATGCCCCTGAACTGGCCAAGGCACATAATAGCCATGCCAGGCCAGAACCACGTC ACCTTCCTGAGAAACAGAATGGCCTCAGTGCAGTGCGGACCATGGAGGCGTTTCACTTTGTCAGCTATGT GCCTATCACAGGACGGCTCTTTGAATTGGATGGGTTGAAAGTCTACCCTATTGATCATGGGCCCTGGGGA GAGGATGAGGAGTGGACAGATAAAGCCCGAAGGGTCATCATGGAGCGAATCGGCCTTGCTACTGCAGGGG AGCCCTACCATGACATTCGCTTCAACCTGATGGCAGTGGTGCCTGACCGCAGGGTTAAGTATGAGGCCAG GCTGCATGTGCTGAAGGGGAACCGACAAACAGTCCTGGAGGCCCTGCAGCAGCTGATTAGAGTAACACAG CCAGAGCTGATTCAGACCCACAAATCTCAAGAGTCACAACTGCCTGAGGAGAGCAAGCCAGCCAGCAGCA AGTCTCCCTTTGGGCTAGAGGCGGGCAGAACCCCGGCGGCCTCTGAGTGCACTCACACAGATGGTGCAGA GGAGGTGGCTGGTTCGTGCCCACAAACTACAACCCATAGTCCTCCCAGCAAATCTAAGCTGGTGGTGAAG CCTTCAGGGAGCAGCCTCAATGGGGTTCCCCCAACCCCTACCCCTATTGTCCAGCGGCTGCCAGCCTTTC TAGATAATCACAATTATGCCAAATCCCCTATGCAGGAGGAGGAAGACCTGGCGGCAGGTGTGGGCCGCAG CCGAGTTCCAGTCCGACCCCAGCAGTACTCTGATGATGAGGAGGACTATGAGGATGAGGAGGAGGATGTG CAGAATACCAGTTCTGCCATCAGATACAAGCGGAAGGGGACAGGGAAGCCAGGATCGTTGAGCAATTCTT CAGATGGGCAGCTGTCAGTGCTGCAGCCCAACACCATCAATGTCTTAACTGAGAAGCTTCAAGAGTCTCA GAAAGACCTTTCAATTCCTCTGTCCATCAAGACTAGCAGTGGGGCTGGGAGTCCAGCCGTGGCTGTGCCC ACACACTCGCAGCCTTCACCCACCCCTAGCAATGAGAGCACGGACACAGCCTCTGAGATTGGCAGTGCTT TCAACTCACCCTTGCGCTCACCCATCCGCTCAGCCAACCCAACACGGCCCTCTAGCCCTGTCACCTCTCA CATCTCCAAGGTGCTCTTTGGAGAAGATGACAGCCTACTTCGTGTTGACTGCATACGCTACAACCGTGCT GTCCGTGACCTGGGTCCTGTCATTAGCACGGGCCTGCTGCACCTTGCTGAGGATGGTGTACTGAGTCCCC TGGCACTCACAGAGGGTGGGAAGGGTTCCTCACCTTCTACCAGATCAAGCCAAGGCAGCCAGGGGTCCAG CAGCCTAGAGGAGAAGGAGGTGGTGGAAGTCACAGATAGCAGAGACAAGTCTGGGCTGAACAGGTCCAGT GAGCCCTTGAGTGGAGAGAAGTACTCGCCCAAGGAGCTGTTAGCACTGTTAAAGTGTGTAGAGGCCGAGA TTGCAAACTATGAGGCCTGTCTCAAGGAGGAGGTGGAGAAAAGGAAGAAGTTCAAGATTGATGACCAGCG AAGGACCCACAACTATGATGAGTTCATCTGCACCTTCATATCCATGCTGGCTCAGGAAGGAATGCTGGCC AACCTGGTGGAGCAGAACATCTCAGTGCGGCGCCGCCAAGGGGTCAGCATTGGTCGGCTTCACAAGCAGC GGAAGCCTGATAGGCGGAAACGCTCTCGCCCCTACAAGGCCAAACGCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001107292 |
| Insert Size | 2223 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001107292.1, NP_001100762.1 |
| RefSeq Size | 3751 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 306257 |
| UniProt ID | D3ZHS6 |
| Gene Summary | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR205856 | Bap1 (Myc-DDK-tagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug) |
CNY 9600.00 |
|
| RR205856L3 | Lenti ORF clone of Bap1 (Myc-DDK-tagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug) |
CNY 11310.00 |
|
| RR205856L4 | Lenti ORF clone of Bap1 (mGFP-tagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug) |
CNY 12350.00 |
