United States
Germany
Japan
United Kingdom
China
Bap1 (NM_001107292) Rat Tagged ORF Clone
CAT＃: RR205856
- TrueORF®
Bap1 (Myc-DDK-tagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug)
"NM_001107292" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205856 representing NM_001107292
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAAGGGCTGGCTGGAGCTGGAGAGTGACCCGGGCCTCTTCACCCTCCTGGTGGAAGATTTCGGTA AGAACCCCTTTACCCCCGATCGTCGGGCCCTGGCTGGTGTCAAAGGGGTGCAAGTGGAGGAGATCTATGA CCTTCAGAGTAAATGCCAGGGGCCTGTATATGGATTTATCTTCCTGTTCAAATGGATCGAAGAGCGCAGG TCCCGCCGCAAGGTTTCTACGTTGGTGGATGATACATCTGTGATTGATGATGACATCGTGAATAGCATGT TCTTTGCTCACCAGCTGATTCCCAACTCTTGTGCCACTCACGCCTTGCTGAGCGTGCTTCTGAACTGCAG CAATGTGGATTTGGGGCCCACGCTGAGTCGAATGAAGGATTTCACCAAAGGCTTCAGCCCTGAGAGCAAA GGATATGCGATTGGCAATGCCCCTGAACTGGCCAAGGCACATAATAGCCATGCCAGGCCAGAACCACGTC ACCTTCCTGAGAAACAGAATGGCCTCAGTGCAGTGCGGACCATGGAGGCGTTTCACTTTGTCAGCTATGT GCCTATCACAGGACGGCTCTTTGAATTGGATGGGTTGAAAGTCTACCCTATTGATCATGGGCCCTGGGGA GAGGATGAGGAGTGGACAGATAAAGCCCGAAGGGTCATCATGGAGCGAATCGGCCTTGCTACTGCAGGGG AGCCCTACCATGACATTCGCTTCAACCTGATGGCAGTGGTGCCTGACCGCAGGGTTAAGTATGAGGCCAG GCTGCATGTGCTGAAGGGGAACCGACAAACAGTCCTGGAGGCCCTGCAGCAGCTGATTAGAGTAACACAG CCAGAGCTGATTCAGACCCACAAATCTCAAGAGTCACAACTGCCTGAGGAGAGCAAGCCAGCCAGCAGCA AGTCTCCCTTTGGGCTAGAGGCGGGCAGAACCCCGGCGGCCTCTGAGTGCACTCACACAGATGGTGCAGA GGAGGTGGCTGGTTCGTGCCCACAAACTACAACCCATAGTCCTCCCAGCAAATCTAAGCTGGTGGTGAAG CCTTCAGGGAGCAGCCTCAATGGGGTTCCCCCAACCCCTACCCCTATTGTCCAGCGGCTGCCAGCCTTTC TAGATAATCACAATTATGCCAAATCCCCTATGCAGGAGGAGGAAGACCTGGCGGCAGGTGTGGGCCGCAG CCGAGTTCCAGTCCGACCCCAGCAGTACTCTGATGATGAGGAGGACTATGAGGATGAGGAGGAGGATGTG CAGAATACCAGTTCTGCCATCAGATACAAGCGGAAGGGGACAGGGAAGCCAGGATCGTTGAGCAATTCTT CAGATGGGCAGCTGTCAGTGCTGCAGCCCAACACCATCAATGTCTTAACTGAGAAGCTTCAAGAGTCTCA GAAAGACCTTTCAATTCCTCTGTCCATCAAGACTAGCAGTGGGGCTGGGAGTCCAGCCGTGGCTGTGCCC ACACACTCGCAGCCTTCACCCACCCCTAGCAATGAGAGCACGGACACAGCCTCTGAGATTGGCAGTGCTT TCAACTCACCCTTGCGCTCACCCATCCGCTCAGCCAACCCAACACGGCCCTCTAGCCCTGTCACCTCTCA CATCTCCAAGGTGCTCTTTGGAGAAGATGACAGCCTACTTCGTGTTGACTGCATACGCTACAACCGTGCT GTCCGTGACCTGGGTCCTGTCATTAGCACGGGCCTGCTGCACCTTGCTGAGGATGGTGTACTGAGTCCCC TGGCACTCACAGAGGGTGGGAAGGGTTCCTCACCTTCTACCAGATCAAGCCAAGGCAGCCAGGGGTCCAG CAGCCTAGAGGAGAAGGAGGTGGTGGAAGTCACAGATAGCAGAGACAAGTCTGGGCTGAACAGGTCCAGT GAGCCCTTGAGTGGAGAGAAGTACTCGCCCAAGGAGCTGTTAGCACTGTTAAAGTGTGTAGAGGCCGAGA TTGCAAACTATGAGGCCTGTCTCAAGGAGGAGGTGGAGAAAAGGAAGAAGTTCAAGATTGATGACCAGCG AAGGACCCACAACTATGATGAGTTCATCTGCACCTTCATATCCATGCTGGCTCAGGAAGGAATGCTGGCC AACCTGGTGGAGCAGAACATCTCAGTGCGGCGCCGCCAAGGGGTCAGCATTGGTCGGCTTCACAAGCAGC GGAAGCCTGATAGGCGGAAACGCTCTCGCCCCTACAAGGCCAAACGCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205856 representing NM_001107292
Red=Cloning site Green=Tags(s) MNKGWLELESDPGLFTLLVEDFGKNPFTPDRRALAGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERR SRRKVSTLVDDTSVIDDDIVNSMFFAHQLIPNSCATHALLSVLLNCSNVDLGPTLSRMKDFTKGFSPESK GYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWG EDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRVKYEARLHVLKGNRQTVLEALQQLIRVTQ PELIQTHKSQESQLPEESKPASSKSPFGLEAGRTPAASECTHTDGAEEVAGSCPQTTTHSPPSKSKLVVK PSGSSLNGVPPTPTPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRPQQYSDDEEDYEDEEEDV QNTSSAIRYKRKGTGKPGSLSNSSDGQLSVLQPNTINVLTEKLQESQKDLSIPLSIKTSSGAGSPAVAVP THSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRA VRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSTRSSQGSQGSSSLEEKEVVEVTDSRDKSGLNRSS EPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLA NLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001107292 |
| ORF Size | 2220 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001107292.1, NP_001100762.1 |
| RefSeq Size | 3751 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 306257 |
| UniProt ID | D3ZHS6 |
| MW | 81.7 kDa |
| Gene Summary | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205856 | Bap1 (untagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug) |
CNY 9,600.00 |
|
| RR205856L3 | Lenti ORF clone of Bap1 (Myc-DDK-tagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug) |
CNY 11,310.00 |
|
| RR205856L4 | Lenti ORF clone of Bap1 (mGFP-tagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), (10 ug) |
CNY 12,350.00 |
