Cacna1d (NM_017298) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208955 representing NM_017298 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGATGATGATGATGAAAAAAATGCAGCATCAACGGCAGCAGCAAGAGGACCACGCGAACGAGG CAAACTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCGAACAGCTCCAA GCAAACCGTCCTGTCTTGGCAAGCTGCAATTGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGC ACGTCTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAAC AGGGTAACTCGTCTAACAGCCGACCTGCCCGTGCCCTCTTCTGTTTATCCCTCAATAACCCCATCCGAAG AGCCTGCATTAGTATAGTGGATTGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGT GTGGCCTTAGCTATTTACATCCCGTTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAG TAGAATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTCCGGACTGCTGCT GCATCCTAATGCTTCCGTTAGGAACGGATGGAACTTACTGGATTTTGTTATAGTAATAGTAGGATTGTTT AGTGTAATTTTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCT TTGATGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCGCTTCGACTTGTATCAGGAGTGCCCAGTTT ACAAGTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTCCACATAGCCCTTTTGGTGTTATTC GTAATTATCATCTATGCGATTATAGGGTTGGAACTTTTTATTGGAAAAATGCACAAAACCTGTTTTTTTG CTGACTCAGATATAGTAGCTGAAGAGGACCCAGCTCCATGCGCGTTCTCAGGGAATGGACGCCAGTGTGC CGCCAATGGCACGGAATGTAGGAGCGGCTGGGTCGGCCCCAATGGAGGCATCACTAATTTTGACAACTTT GCCTTTGCCATGCTCACTGTGTTCCAGTGCATCACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTA ATGATGCGATAGGATGGGAGTGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGT CCTTAACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCAAAGGAAAGAGAGAAGGCTAAAGCACGTGGA GATTTCCAGAAGCTTCGGGAGAAGCAGCAGCTGGAAGAGGATCTCAAGGGCTACTTGGACTGGATCACCC AGGCAGAAGACATAGATCCTGAGAATGAAGAGGAGGGTGGAGAGGAGGGCAAACGAAACACTAGCATGCC CACCAGTGAGACCGAATCTGTGAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGA AGTCTCTGGTGCTGGTGGAAAAGAAGAGGCGCGGCCAAGACTGGGCCCTCTGGGTGTCGGCGGTGGGGCC AAGCCATCTCGAAATCCAAGCTCAGATCGCACGGGGCCCGCGAGGCTTTGTGTGTGTGCCGGTGCAGCCT TGAATCTCTCGTGAAACTATGGACGTCTAGGTTCTCAGCCCACTTGCAGGCTGCATATGTCAGACCCTAC AGCCGTCGGTGGCGTCGCTGGAACCGGTTCAATCGCAGGAGATGCAGGGCAGCTGTGAAGTCTGTCACGT TTTACTGGCTGGTGATCGTTCTGGTGTTTCTCAACACTCTGACGATTTCCTCTGAGCACTACAATCAGCC AGACTGGCTGACGCAGATTCAAGATATAGCGAACAAAGTGCTCTTGGCTTTGTTCACCTGTGAGATGCTG GTGAAGATGTACAGCCTGGGCCTCCAGGCCTACTTCGTCTCTCTCTTCAACCGCTTCGATTGCTTTGTGG TGTGTGGGGGAATCACCGAAACCATCCTGGTGGAACTGGAGCTCATGTCTCCCCTGGGCGTCTCTGTGTT CCGGTGTGTACGTCTCCTGAGGATCTTCAAAGTGACCAGGCACTGGACTTCCCTGAGCAACTTGGTGGCG TCCCTGTTAAACTCCATGAAGTCCATCGCCTCTCTGCTGTTGCTGCTCTTCCTCTTCATCATCATCTTCT CCCTGCTGGGGATGCAGCTGTTCGGAGGGAAGTTCAATTTCGATGAGACACAGACCAAGCGAAGCACCTT CGACAACTTCCCACAGGCGCTCCTGACTGTGTTCCAGATCCTGACGGGCGAAGACTGGAATGCGGTGATG TATGACGGCATCATGGCCTACGGAGGCCCGTCCTCCTCTGGAATGATTGTCTGTATTTACTTCATCATCC TTTTCATCTGTGGTAACTATATCCTGCTGAAGCTCTTCTTGGCCATTGCTGTAGACAATTTGGCTGATGC TGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCCGAAGAAAAAGAAAGGAAAAAAATTGCCAGAAAAGAA AGCCTAGAAAACAAAAAAAACAACAAACCAGAGGTCAACCAGATAGCCAACAGTGACAACAAGGTTACTA TTGATGACTATCAAGAGGAGGCTGAAGATAAGGACCCTTATCCACCCTGCGATGTGCCAGTAGGTGAAGA GGAAGAGGAGGAGGAAGAGGACGAGCCTGAGGTTCCTGCTGGTCCCCGTCCTCGAAGAATCTCAGAGTTG AACATGAAGGAGAAAATTGCGCCCATCCCCGAAGGAAGCGCATTCTTCATTCTTAGCAAGACCAACCCGA TCCGTGTGGGTTGCCACAAACTCATCAACCACCACATCTTCACCAACCTCATCCTGGTCTTCATCATGCT GAGCAGCGCTGCCCTGGCCGCAGAGGATCCCATCCGCAGCCACTCCTTCCGCAACACTATACTGGGCTAC TTTGACTATGCTTTCACAGCCATCTTTACGGTTGAAATCCTGTTAAAGATGACAACTTTTGGAGCGTTCT TGCACAAAGGGGCTTTCTGTAGGAACTACTTCAATTTGCTGGACATGCTGGTCGTTGGGGTGTCTCTGGT GTCATTTGGGATTCAATCCAGTGCCATCTCGGTTGTGAAGATTCTGAGGGTCTTAAGGGTCTTGAGGCCT CTCAGAGCAATCAACAGAGCAAAAGGACTTAAGCACGTGGTCCAGTGTGTCTTTGTGGCCATCCGAACCA TCGGCAACATCATGATCGTCACGACCCTGCTCCAGTTCATGTTTGCTTGCATTGGGGTCCAGCTGTTCAA GGGGAAGTTCTACCGTTGCACAGATGAGGCCAAAAGTAACCCCGAGGAGTGCAGGGGGCTTTTCATCCTT TATAAGGACGGCGATGTCGACAGTCCCGTGGTCCGTGAGAGGATCTGGCAAAACAGTGATTTCAATTTCG ACAATGTCCTTTCGGCTATGATGGCGCTCTTCACGGTCTCGACTTTTGAGGGCTGGCCCGCGTTGCTGTA CAAAGCTATCGACTCAAACGGAGAGAACGTTGGTCCTGTCTACAACTACCGTGTGGAGATCTCCATCTTC TTCATTATCTACATCATCATCGTGGCCTTCTTCATGATGAATATCTTCGTGGGCTTCGTCATCGTCACCT TCCAGGAGCAGGGAGAAAAGGAGTATAAGAACTGTGAGCTGGACAAAAATCAGCGTCAGTGTGTGGAATA TGCCTTGAAGGCCCGCCCCTTAAGGAGATACATCCCCAAAAACCCATACCAGTACAAGTTCTGGTACGTG GTGAACTCCTCGCCTTTCGAATATATGATGTTTGTCCTCATCATGCTCAACACGCTCTGCCTGGCCATGC AGCACTATGAGCAATCCAAGATGTTCAATGACGCCATGGACATTCTGAACATGGTCTTCACGGGGGTCTT CACCGTTGAGATGGTTTTGAAAGTCATCGCATTTAAGCCCAAGGGGTATTTTAGTGACGCCTGGAACACG TTTGACTCCCTCATCGTAATCGGCAGCATTATAGACGTGGCACTCAGCGAAGCTGACAACTCTGAAGAGA GCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTGATGCGGTTGGTGAAGCTTCTCAGCAGAGG GGAAGGCATCCGGACTCTGCTATGGACCTTCATTAAGTCCTTCCAGGCACTCCCATATGTCGCCCTCCTC ATTGCCATGCTCTTCTTCATCTACGCCGTCATTGGCATGCAGATGTTTGGGAAGGTTGCCATGAGAGATA ACAACCAGATCAATAGGAACAACAACTTCCAGACGTTTCCCCAGGCAGTGCTGCTGCTCTTCAGGTGTGC AACAGGGGAGGCCTGGCAGGAGATCATGCTCGCCTGCCTCCCTGGGAAGCTGTGTGACCCGGACTCAGAT TACAACCCAGGAGAGGAATATACTTGTGGGAGCAACTTTGCCATTGTCTACTTCATCAGCTTTTACATGC TCTGTGCGTTCCTGATCATCAACCTCTTCGTGGCTGTCATCATGGACAATTTTGACTATCTGACGAGGGA CTGGTCTATTCTGGGGCCTCACCACTTGGACGAATTCAAAAGAATATGGTCTGAATATGACCCCGAGGCA AAGGGAAGGATAAAACACCTTGATGTGGTCACTTTGCTCCGACGTATCCAGCCTCCCCTGGGATTTGGAA AGTTGTGTCCACACCGAGTGGCGTGTAAGAGATTGGTCGCCATGAACATGCCTCTCAACAGTGATGGGAC AGTCATGTTCAATGCAACTCTGTTTGCTTTGGTACGGACGGCTCTCAAGATCAAGACTGAAGGCAACCTG GAGCAAGCTAATGAAGAGCTCCGCGCTGTGATAAAGAAAATCTGGAAGAAGACAAGCATGAAGCTACTTG ACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTGGGGAAGTTCTATGCCACTTTCCTGATACA GGACTACTTTAGGAAATTCAAGAAGCGGAAAGAGCAAGGCCTGGTGGGGAAATACCCTGCGAAGAACACC ACGATCGCCCTACAGGCGGGATTAAGGACCCTGCATGACATTGGGCCAGAAATCCGACGGGCTATATCCT GTGACTTGCAAGATGACGAACCAGAAGACTCCAAACCAGAAGAGGAAGATGTATTCAAAAGAAATGGTGC CCTGCTTGGAAACTATGTCAATCATGTTAATAGTGATAGGAGAGAGTCCCTTCAGCAGACCAATACCACC CACCGTCCCCTGCATGTCCAAAGGCCTTCAATTCCACCTGCAAGTGATACTGAGAAACCGCTGTTTCCTC CAGCAGGAAATTCGGTGTGTCATAACCATCATAACCATAATTCCATAGGGAAGCAAGTTCCCACCTCCAC AAATGCCAATCTCAATAACGCCAATATGTCCAAAGCTGCCCACGGAAAGCGGCCCAGCATTGGGGACCTT GAGCATGTGTCTGAAAACGGCCATTACTCCTACAAGCACGACCGAGAGCTTCAAAGAAGGTCCAGTATTA AAAGAACCCGCTATTATGAAACTTACATTAGGTCTGAGTCAGGAGACGAGCAGCTTCCAACCATTTTCCG TGAAGACCCTGAGATACACGGCTACTTCCGAGACCCCCGCTGCTTCGGGGAGCAGGAATATTTCAGTAGC GAGGAGTGCTGTGAGGATGACAGTTCTCCCACCTGGAGCAGGCAAAACTACAGCTATTACAACAGGTACC CAGGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTACCATCACCCCCAAGGTTTCTTAGAAGATGACGA CTCTCCCATTGGCTATGACTCACGGAGATCTCCAAGAAGACGCCTTCTACCTCCTACCCCACCATCCCAT CGGAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGCAAAACAGCCAAGATGACGTCCTTCCGTCGCCTG CCCTGCCTCACCGCGCTGCCCTGCCCCTGCACCTTATGCAGCAGCAGATCATGGCCGTTGCAGGCCTAGA TTCCAGTAAAGCCCAGAAGTACTCGCCGAGCCACTCTACCCGGTCATGGGCCACCCCTCCAGCAACCCCT CCGTACCGAGACTGGACCCCATGCTACACCCCTTTGATCCAGGTGGATCGCTCAGAGTCTATGGACCAGG TCAATGGCAGCCTGCCGTCGCTACACCGCAGTTCCTGGTATACAGATGAACCCGACATCTCCTATAGGAC TTTCACACCGGCCAGCCTGACCGTCCCCAGCAGCTTCCGGAACAAAAACAGCGACAAGCAGAGGAGTGCA GACAGCCTGGTGGAAGCAGTCCTGATATCCGAAGGCTTGGGACGCTACGCAAGGGACCCAAAATTTGTCT CAGCAACAAAACATGAGATCGCCGATGCCTGTGACCTAACCATAGACGAGATGGAGAGCGCGGCCAGCAC CCTGCTCAATGGAAGCGTGTGTCCTCGAGCCAACGGGGACATGGGCCCCATCTCACACCGCCAGGACTAT GAGCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGGAACCAGACCCTGGGCGAGAGGAAGAGGACCTGG CAGATGAAATGATTTGCATCACCACCTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017298
Insert Size	6612 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017298.1, NP_058994.1
RefSeq Size	7986 bp
RefSeq ORF	6612 bp
Locus ID	29716
UniProt ID	P27732
Gene Summary	has high voltage L-type calcium channel activity that is sensitive to dihydropyridines [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.