Cacna1d (NM_017298) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208955 representing NM_017298 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGATGATGATGATGAAAAAAATGCAGCATCAACGGCAGCAGCAAGAGGACCACGCGAACGAGG CAAACTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCGAACAGCTCCAA GCAAACCGTCCTGTCTTGGCAAGCTGCAATTGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGC ACGTCTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAAC AGGGTAACTCGTCTAACAGCCGACCTGCCCGTGCCCTCTTCTGTTTATCCCTCAATAACCCCATCCGAAG AGCCTGCATTAGTATAGTGGATTGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGT GTGGCCTTAGCTATTTACATCCCGTTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAG TAGAATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTCCGGACTGCTGCT GCATCCTAATGCTTCCGTTAGGAACGGATGGAACTTACTGGATTTTGTTATAGTAATAGTAGGATTGTTT AGTGTAATTTTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCT TTGATGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCGCTTCGACTTGTATCAGGAGTGCCCAGTTT ACAAGTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTCCACATAGCCCTTTTGGTGTTATTC GTAATTATCATCTATGCGATTATAGGGTTGGAACTTTTTATTGGAAAAATGCACAAAACCTGTTTTTTTG CTGACTCAGATATAGTAGCTGAAGAGGACCCAGCTCCATGCGCGTTCTCAGGGAATGGACGCCAGTGTGC CGCCAATGGCACGGAATGTAGGAGCGGCTGGGTCGGCCCCAATGGAGGCATCACTAATTTTGACAACTTT GCCTTTGCCATGCTCACTGTGTTCCAGTGCATCACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTA ATGATGCGATAGGATGGGAGTGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGT CCTTAACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCAAAGGAAAGAGAGAAGGCTAAAGCACGTGGA GATTTCCAGAAGCTTCGGGAGAAGCAGCAGCTGGAAGAGGATCTCAAGGGCTACTTGGACTGGATCACCC AGGCAGAAGACATAGATCCTGAGAATGAAGAGGAGGGTGGAGAGGAGGGCAAACGAAACACTAGCATGCC CACCAGTGAGACCGAATCTGTGAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGA AGTCTCTGGTGCTGGTGGAAAAGAAGAGGCGCGGCCAAGACTGGGCCCTCTGGGTGTCGGCGGTGGGGCC AAGCCATCTCGAAATCCAAGCTCAGATCGCACGGGGCCCGCGAGGCTTTGTGTGTGTGCCGGTGCAGCCT TGAATCTCTCGTGAAACTATGGACGTCTAGGTTCTCAGCCCACTTGCAGGCTGCATATGTCAGACCCTAC AGCCGTCGGTGGCGTCGCTGGAACCGGTTCAATCGCAGGAGATGCAGGGCAGCTGTGAAGTCTGTCACGT TTTACTGGCTGGTGATCGTTCTGGTGTTTCTCAACACTCTGACGATTTCCTCTGAGCACTACAATCAGCC AGACTGGCTGACGCAGATTCAAGATATAGCGAACAAAGTGCTCTTGGCTTTGTTCACCTGTGAGATGCTG GTGAAGATGTACAGCCTGGGCCTCCAGGCCTACTTCGTCTCTCTCTTCAACCGCTTCGATTGCTTTGTGG TGTGTGGGGGAATCACCGAAACCATCCTGGTGGAACTGGAGCTCATGTCTCCCCTGGGCGTCTCTGTGTT CCGGTGTGTACGTCTCCTGAGGATCTTCAAAGTGACCAGGCACTGGACTTCCCTGAGCAACTTGGTGGCG TCCCTGTTAAACTCCATGAAGTCCATCGCCTCTCTGCTGTTGCTGCTCTTCCTCTTCATCATCATCTTCT CCCTGCTGGGGATGCAGCTGTTCGGAGGGAAGTTCAATTTCGATGAGACACAGACCAAGCGAAGCACCTT CGACAACTTCCCACAGGCGCTCCTGACTGTGTTCCAGATCCTGACGGGCGAAGACTGGAATGCGGTGATG TATGACGGCATCATGGCCTACGGAGGCCCGTCCTCCTCTGGAATGATTGTCTGTATTTACTTCATCATCC TTTTCATCTGTGGTAACTATATCCTGCTGAAGCTCTTCTTGGCCATTGCTGTAGACAATTTGGCTGATGC TGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCCGAAGAAAAAGAAAGGAAAAAAATTGCCAGAAAAGAA AGCCTAGAAAACAAAAAAAACAACAAACCAGAGGTCAACCAGATAGCCAACAGTGACAACAAGGTTACTA TTGATGACTATCAAGAGGAGGCTGAAGATAAGGACCCTTATCCACCCTGCGATGTGCCAGTAGGTGAAGA GGAAGAGGAGGAGGAAGAGGACGAGCCTGAGGTTCCTGCTGGTCCCCGTCCTCGAAGAATCTCAGAGTTG AACATGAAGGAGAAAATTGCGCCCATCCCCGAAGGAAGCGCATTCTTCATTCTTAGCAAGACCAACCCGA TCCGTGTGGGTTGCCACAAACTCATCAACCACCACATCTTCACCAACCTCATCCTGGTCTTCATCATGCT GAGCAGCGCTGCCCTGGCCGCAGAGGATCCCATCCGCAGCCACTCCTTCCGCAACACTATACTGGGCTAC TTTGACTATGCTTTCACAGCCATCTTTACGGTTGAAATCCTGTTAAAGATGACAACTTTTGGAGCGTTCT TGCACAAAGGGGCTTTCTGTAGGAACTACTTCAATTTGCTGGACATGCTGGTCGTTGGGGTGTCTCTGGT GTCATTTGGGATTCAATCCAGTGCCATCTCGGTTGTGAAGATTCTGAGGGTCTTAAGGGTCTTGAGGCCT CTCAGAGCAATCAACAGAGCAAAAGGACTTAAGCACGTGGTCCAGTGTGTCTTTGTGGCCATCCGAACCA TCGGCAACATCATGATCGTCACGACCCTGCTCCAGTTCATGTTTGCTTGCATTGGGGTCCAGCTGTTCAA GGGGAAGTTCTACCGTTGCACAGATGAGGCCAAAAGTAACCCCGAGGAGTGCAGGGGGCTTTTCATCCTT TATAAGGACGGCGATGTCGACAGTCCCGTGGTCCGTGAGAGGATCTGGCAAAACAGTGATTTCAATTTCG ACAATGTCCTTTCGGCTATGATGGCGCTCTTCACGGTCTCGACTTTTGAGGGCTGGCCCGCGTTGCTGTA CAAAGCTATCGACTCAAACGGAGAGAACGTTGGTCCTGTCTACAACTACCGTGTGGAGATCTCCATCTTC TTCATTATCTACATCATCATCGTGGCCTTCTTCATGATGAATATCTTCGTGGGCTTCGTCATCGTCACCT TCCAGGAGCAGGGAGAAAAGGAGTATAAGAACTGTGAGCTGGACAAAAATCAGCGTCAGTGTGTGGAATA TGCCTTGAAGGCCCGCCCCTTAAGGAGATACATCCCCAAAAACCCATACCAGTACAAGTTCTGGTACGTG GTGAACTCCTCGCCTTTCGAATATATGATGTTTGTCCTCATCATGCTCAACACGCTCTGCCTGGCCATGC AGCACTATGAGCAATCCAAGATGTTCAATGACGCCATGGACATTCTGAACATGGTCTTCACGGGGGTCTT CACCGTTGAGATGGTTTTGAAAGTCATCGCATTTAAGCCCAAGGGGTATTTTAGTGACGCCTGGAACACG TTTGACTCCCTCATCGTAATCGGCAGCATTATAGACGTGGCACTCAGCGAAGCTGACAACTCTGAAGAGA GCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTGATGCGGTTGGTGAAGCTTCTCAGCAGAGG GGAAGGCATCCGGACTCTGCTATGGACCTTCATTAAGTCCTTCCAGGCACTCCCATATGTCGCCCTCCTC ATTGCCATGCTCTTCTTCATCTACGCCGTCATTGGCATGCAGATGTTTGGGAAGGTTGCCATGAGAGATA ACAACCAGATCAATAGGAACAACAACTTCCAGACGTTTCCCCAGGCAGTGCTGCTGCTCTTCAGGTGTGC AACAGGGGAGGCCTGGCAGGAGATCATGCTCGCCTGCCTCCCTGGGAAGCTGTGTGACCCGGACTCAGAT TACAACCCAGGAGAGGAATATACTTGTGGGAGCAACTTTGCCATTGTCTACTTCATCAGCTTTTACATGC TCTGTGCGTTCCTGATCATCAACCTCTTCGTGGCTGTCATCATGGACAATTTTGACTATCTGACGAGGGA CTGGTCTATTCTGGGGCCTCACCACTTGGACGAATTCAAAAGAATATGGTCTGAATATGACCCCGAGGCA AAGGGAAGGATAAAACACCTTGATGTGGTCACTTTGCTCCGACGTATCCAGCCTCCCCTGGGATTTGGAA AGTTGTGTCCACACCGAGTGGCGTGTAAGAGATTGGTCGCCATGAACATGCCTCTCAACAGTGATGGGAC AGTCATGTTCAATGCAACTCTGTTTGCTTTGGTACGGACGGCTCTCAAGATCAAGACTGAAGGCAACCTG GAGCAAGCTAATGAAGAGCTCCGCGCTGTGATAAAGAAAATCTGGAAGAAGACAAGCATGAAGCTACTTG ACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTGGGGAAGTTCTATGCCACTTTCCTGATACA GGACTACTTTAGGAAATTCAAGAAGCGGAAAGAGCAAGGCCTGGTGGGGAAATACCCTGCGAAGAACACC ACGATCGCCCTACAGGCGGGATTAAGGACCCTGCATGACATTGGGCCAGAAATCCGACGGGCTATATCCT GTGACTTGCAAGATGACGAACCAGAAGACTCCAAACCAGAAGAGGAAGATGTATTCAAAAGAAATGGTGC CCTGCTTGGAAACTATGTCAATCATGTTAATAGTGATAGGAGAGAGTCCCTTCAGCAGACCAATACCACC CACCGTCCCCTGCATGTCCAAAGGCCTTCAATTCCACCTGCAAGTGATACTGAGAAACCGCTGTTTCCTC CAGCAGGAAATTCGGTGTGTCATAACCATCATAACCATAATTCCATAGGGAAGCAAGTTCCCACCTCCAC AAATGCCAATCTCAATAACGCCAATATGTCCAAAGCTGCCCACGGAAAGCGGCCCAGCATTGGGGACCTT GAGCATGTGTCTGAAAACGGCCATTACTCCTACAAGCACGACCGAGAGCTTCAAAGAAGGTCCAGTATTA AAAGAACCCGCTATTATGAAACTTACATTAGGTCTGAGTCAGGAGACGAGCAGCTTCCAACCATTTTCCG TGAAGACCCTGAGATACACGGCTACTTCCGAGACCCCCGCTGCTTCGGGGAGCAGGAATATTTCAGTAGC GAGGAGTGCTGTGAGGATGACAGTTCTCCCACCTGGAGCAGGCAAAACTACAGCTATTACAACAGGTACC CAGGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTACCATCACCCCCAAGGTTTCTTAGAAGATGACGA CTCTCCCATTGGCTATGACTCACGGAGATCTCCAAGAAGACGCCTTCTACCTCCTACCCCACCATCCCAT CGGAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGCAAAACAGCCAAGATGACGTCCTTCCGTCGCCTG CCCTGCCTCACCGCGCTGCCCTGCCCCTGCACCTTATGCAGCAGCAGATCATGGCCGTTGCAGGCCTAGA TTCCAGTAAAGCCCAGAAGTACTCGCCGAGCCACTCTACCCGGTCATGGGCCACCCCTCCAGCAACCCCT CCGTACCGAGACTGGACCCCATGCTACACCCCTTTGATCCAGGTGGATCGCTCAGAGTCTATGGACCAGG TCAATGGCAGCCTGCCGTCGCTACACCGCAGTTCCTGGTATACAGATGAACCCGACATCTCCTATAGGAC TTTCACACCGGCCAGCCTGACCGTCCCCAGCAGCTTCCGGAACAAAAACAGCGACAAGCAGAGGAGTGCA GACAGCCTGGTGGAAGCAGTCCTGATATCCGAAGGCTTGGGACGCTACGCAAGGGACCCAAAATTTGTCT CAGCAACAAAACATGAGATCGCCGATGCCTGTGACCTAACCATAGACGAGATGGAGAGCGCGGCCAGCAC CCTGCTCAATGGAAGCGTGTGTCCTCGAGCCAACGGGGACATGGGCCCCATCTCACACCGCCAGGACTAT GAGCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGGAACCAGACCCTGGGCGAGAGGAAGAGGACCTGG CAGATGAAATGATTTGCATCACCACCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208955 representing NM_017298 Red=Cloning site Green=Tags(s) MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMS TSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANC VALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIASGLLLHPNASVRNGWNLLDFVIVIVGLF SVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLF VIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNF AFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARG DFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCG SLWCWWKRRGAAKTGPSGCRRWGQAISKSKLRSHGAREALCVCRCSLESLVKLWTSRFSAHLQAAYVRPY SRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEML VKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVA SLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVM YDGIMAYGGPSSSGMIVCIYFIILFICGNYILLKLFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKE SLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISEL NMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGY FDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRP LRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFIL YKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIF FIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYV VNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNT FDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALL IAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSD YNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEA KGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNL EQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNT TIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTT HRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDL EHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTIFREDPEIHGYFRDPRCFGEQEYFSS EECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSH RRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATP PYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSA DSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDY ELQDFGPGYSDEEPDPGREEEDLADEMICITTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017298
ORF Size	6609 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017298.1, NP_058994.1
RefSeq Size	7986 bp
RefSeq ORF	6612 bp
Locus ID	29716
UniProt ID	P27732
MW	250.1 kDa
Gene Summary	has high voltage L-type calcium channel activity that is sensitive to dihydropyridines [RGD, Feb 2006]

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